FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4282832333> ?p ?o ?g. }

• W4282832333 endingPage "11" @default.
• W4282832333 startingPage "1" @default.
• W4282832333 abstract "Background. A growing number of studies have shown that long noncoding RNAs play an important role in osteoclast differentiation. However, there are few studies on the roles of lncRNA small nucleolar RNA host gene 15 (SNHG15) in osteoclast differentiation. Methods. The expressions of SNHG15, miR-381-3p, and never in mitosis-related kinase 2 (NEK2) mRNA were detected by real-time quantitative polymerase chain reaction (RT-qPCR); Western blot detected NEK2 and osteoclast markers (Cathepsin K, CTSK), matrix metalloproteinase 9 (MMP9), nuclear factor of activated T cell 2 (NFAT2), and tartrate-resistant acid phosphatase (TRAP) protein levels; cell proliferation was detected by Cell Counting Kit-8 (CCK-8), and the formation of osteoclasts was observed by TRAP staining; the F-actin skeleton was stained with tetramethylrhodamine isothiocyanate (TRITC) phalloidin; cell migration rate was detected by Transwell; dual-luciferase reporter gene assay and RNA-binding protein immunoprecipitation (RIP) assay verified the targeting relationship between miR-381-3p, SNHG15, and NEK2. Results. The expression of SNHG15 was increased in THP-1 cells stimulated by macrophage colony-stimulating factor (M-CSF)/receptor activator of nuclear factor-kappa B ligand (RANKL). Overexpression of SNHG15 significantly promoted the proliferation, migration, osteoclast differentiation, and expression of osteoclast markers CTSK, MMP9, NFAT2, and TRAP of THP-1 cells induced by M-CSF/RANKL. Knockdown of SNHG15 reversed this effect. Overexpression of SNHG15 downregulated the inhibitory effect of overexpression of miR-381-3p on the proliferation, migration, and differentiation of THP-1 cells induced by M-CSF/RANKL. Knockdown of miR-381-3p reversed the inhibitory effect of knockdown of NEK2 on the proliferation, migration, and differentiation of THP-1 cells induced by M-CSF/RANKL. Conclusion. SNHG15 acted as a ceRNA promoted the proliferation, migration, and differentiation of THP-1 cells induced by M-CSF/RANKL through sponging miR-381-3p to promote the expression of NEK2." @default.
• W4282832333 created "2022-06-15" @default.
• W4282832333 creator A5008405961 @default.
• W4282832333 creator A5031063585 @default.
• W4282832333 creator A5044627178 @default.
• W4282832333 creator A5060888542 @default.
• W4282832333 date "2022-06-12" @default.
• W4282832333 modified "2023-10-18" @default.
• W4282832333 title "lncRNA SNHG15 as a ceRNA modulates Osteoclast Differentiation, Proliferation, and Metastasis by Sponging miR-381-3p/NEK2 Axis" @default.
• W4282832333 cites W2087665466 @default.
• W4282832333 cites W2299414460 @default.
• W4282832333 cites W2606428688 @default.
• W4282832333 cites W2786621229 @default.
• W4282832333 cites W2789781781 @default.
• W4282832333 cites W2859074008 @default.
• W4282832333 cites W2886585246 @default.
• W4282832333 cites W2888013217 @default.
• W4282832333 cites W2937508182 @default.
• W4282832333 cites W2953382719 @default.
• W4282832333 cites W2961940080 @default.
• W4282832333 cites W2970937984 @default.
• W4282832333 cites W2998836461 @default.
• W4282832333 cites W3004356133 @default.
• W4282832333 cites W3004580090 @default.
• W4282832333 cites W3010405431 @default.
• W4282832333 cites W3023562097 @default.
• W4282832333 cites W3024714536 @default.
• W4282832333 cites W3041612122 @default.
• W4282832333 cites W3044089550 @default.
• W4282832333 cites W3081307951 @default.
• W4282832333 cites W3083896203 @default.
• W4282832333 cites W3083952170 @default.
• W4282832333 cites W3093153931 @default.
• W4282832333 cites W3105294284 @default.
• W4282832333 cites W3119130184 @default.
• W4282832333 cites W3124588260 @default.
• W4282832333 cites W3132241295 @default.
• W4282832333 cites W3138527422 @default.
• W4282832333 cites W3161978623 @default.
• W4282832333 cites W3165023024 @default.
• W4282832333 cites W3177256224 @default.
• W4282832333 cites W3188283582 @default.
• W4282832333 cites W3203293249 @default.
• W4282832333 cites W3208070846 @default.
• W4282832333 cites W4211083916 @default.
• W4282832333 doi "https://doi.org/10.1155/2022/8634820" @default.
• W4282832333 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35733923" @default.
• W4282832333 hasPublicationYear "2022" @default.
• W4282832333 type Work @default.
• W4282832333 citedByCount "3" @default.
• W4282832333 countsByYear W42828323332022 @default.
• W4282832333 countsByYear W42828323332023 @default.
• W4282832333 crossrefType "journal-article" @default.
• W4282832333 hasAuthorship W4282832333A5008405961 @default.
• W4282832333 hasAuthorship W4282832333A5031063585 @default.
• W4282832333 hasAuthorship W4282832333A5044627178 @default.
• W4282832333 hasAuthorship W4282832333A5060888542 @default.
• W4282832333 hasBestOaLocation W42828323331 @default.
• W4282832333 hasConcept C101990758 @default.
• W4282832333 hasConcept C104317684 @default.
• W4282832333 hasConcept C148738053 @default.
• W4282832333 hasConcept C153911025 @default.
• W4282832333 hasConcept C170493617 @default.
• W4282832333 hasConcept C173396325 @default.
• W4282832333 hasConcept C185592680 @default.
• W4282832333 hasConcept C190283241 @default.
• W4282832333 hasConcept C2776033226 @default.
• W4282832333 hasConcept C2779428903 @default.
• W4282832333 hasConcept C55493867 @default.
• W4282832333 hasConcept C62112901 @default.
• W4282832333 hasConcept C86803240 @default.
• W4282832333 hasConcept C88045685 @default.
• W4282832333 hasConcept C95444343 @default.
• W4282832333 hasConceptScore W4282832333C101990758 @default.
• W4282832333 hasConceptScore W4282832333C104317684 @default.
• W4282832333 hasConceptScore W4282832333C148738053 @default.
• W4282832333 hasConceptScore W4282832333C153911025 @default.
• W4282832333 hasConceptScore W4282832333C170493617 @default.
• W4282832333 hasConceptScore W4282832333C173396325 @default.
• W4282832333 hasConceptScore W4282832333C185592680 @default.
• W4282832333 hasConceptScore W4282832333C190283241 @default.
• W4282832333 hasConceptScore W4282832333C2776033226 @default.
• W4282832333 hasConceptScore W4282832333C2779428903 @default.
• W4282832333 hasConceptScore W4282832333C55493867 @default.
• W4282832333 hasConceptScore W4282832333C62112901 @default.
• W4282832333 hasConceptScore W4282832333C86803240 @default.
• W4282832333 hasConceptScore W4282832333C88045685 @default.
• W4282832333 hasConceptScore W4282832333C95444343 @default.
• W4282832333 hasFunder F4320322922 @default.
• W4282832333 hasLocation W42828323331 @default.
• W4282832333 hasLocation W42828323332 @default.
• W4282832333 hasLocation W42828323333 @default.
• W4282832333 hasOpenAccess W4282832333 @default.
• W4282832333 hasPrimaryLocation W42828323331 @default.
• W4282832333 hasRelatedWork W1993812666 @default.
• W4282832333 hasRelatedWork W1998745604 @default.
• W4282832333 hasRelatedWork W2074483736 @default.
• W4282832333 hasRelatedWork W2142016853 @default.

• next