FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4282942641> ?p ?o ?g. }

• W4282942641 endingPage "3067" @default.
• W4282942641 startingPage "3059" @default.
• W4282942641 abstract "Extrachromosomal circular DNA (eccDNA) of chromosomal origin is common in eukaryotic cells. Amplification of oncogenes on large eccDNA (ecDNA) can drive biological processes such as tumorigenesis, and identification of eccDNA by sequencing after removal of chromosomal DNA is therefore important for understanding their impact on the expressed phenotype. However, the circular mitochondrial DNA (mtDNA) might challenge the detection of eccDNA because the average somatic cell has hundreds of copies of mtDNA. Here we show that 61.2-99.5% of reads from eccDNA-enriched samples correspond to mtDNA in mouse tissues. We have developed a method to selectively remove mtDNA from total circular DNA by CRISPR/Cas9 guided cleavage of mtDNA with one single-guide RNA (sgRNA) or two sgRNAs followed by exonuclease degradation of the linearized mtDNA. Sequencing revealed that mtDNA reads were 85.9% ± 12.6% removed from eccDNA of 9 investigated mouse tissues. CRISPR/Cas9 cleavage also efficiently removed mtDNA from a human HeLa cell line and colorectal cancer samples. We identified up to 14 times more, and also larger eccDNA in CRISPR/Cas9 treated colorectal cancer samples than in untreated samples. We foresee that the method can be applied to effectively remove mtDNA from any eukaryotic species to obtain higher eccDNA yields." @default.
• W4282942641 created "2022-06-16" @default.
• W4282942641 creator A5005444139 @default.
• W4282942641 creator A5014329497 @default.
• W4282942641 creator A5015240577 @default.
• W4282942641 creator A5019059200 @default.
• W4282942641 creator A5033748161 @default.
• W4282942641 creator A5039041871 @default.
• W4282942641 creator A5073216193 @default.
• W4282942641 creator A5073734633 @default.
• W4282942641 creator A5079972363 @default.
• W4282942641 date "2022-01-01" @default.
• W4282942641 modified "2023-09-29" @default.
• W4282942641 title "Targeted removal of mitochondrial DNA from mouse and human extrachromosomal circular DNA with CRISPR-Cas9" @default.
• W4282942641 cites W1569227072 @default.
• W4282942641 cites W1963578989 @default.
• W4282942641 cites W1988582871 @default.
• W4282942641 cites W1996943057 @default.
• W4282942641 cites W2004691672 @default.
• W4282942641 cites W2005115865 @default.
• W4282942641 cites W2010842439 @default.
• W4282942641 cites W2019670881 @default.
• W4282942641 cites W2058123255 @default.
• W4282942641 cites W2061329995 @default.
• W4282942641 cites W2064815984 @default.
• W4282942641 cites W2101802274 @default.
• W4282942641 cites W2108234281 @default.
• W4282942641 cites W2153344788 @default.
• W4282942641 cites W2332807235 @default.
• W4282942641 cites W2344328043 @default.
• W4282942641 cites W2513264630 @default.
• W4282942641 cites W2547365070 @default.
• W4282942641 cites W2587686129 @default.
• W4282942641 cites W2783521900 @default.
• W4282942641 cites W2790044474 @default.
• W4282942641 cites W2792321518 @default.
• W4282942641 cites W2888581595 @default.
• W4282942641 cites W2897507122 @default.
• W4282942641 cites W2912484801 @default.
• W4282942641 cites W2941449697 @default.
• W4282942641 cites W2991552764 @default.
• W4282942641 cites W2992452894 @default.
• W4282942641 cites W2997236115 @default.
• W4282942641 cites W2997406759 @default.
• W4282942641 cites W3003871524 @default.
• W4282942641 cites W3014602217 @default.
• W4282942641 cites W3015398789 @default.
• W4282942641 cites W3017249887 @default.
• W4282942641 cites W3024840754 @default.
• W4282942641 cites W3049406491 @default.
• W4282942641 cites W3158516617 @default.
• W4282942641 cites W3159756243 @default.
• W4282942641 cites W3207537581 @default.
• W4282942641 cites W330238655 @default.
• W4282942641 cites W4225716595 @default.
• W4282942641 doi "https://doi.org/10.1016/j.csbj.2022.06.028" @default.
• W4282942641 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35782732" @default.
• W4282942641 hasPublicationYear "2022" @default.
• W4282942641 type Work @default.
• W4282942641 citedByCount "7" @default.
• W4282942641 countsByYear W42829426412022 @default.
• W4282942641 countsByYear W42829426412023 @default.
• W4282942641 crossrefType "journal-article" @default.
• W4282942641 hasAuthorship W4282942641A5005444139 @default.
• W4282942641 hasAuthorship W4282942641A5014329497 @default.
• W4282942641 hasAuthorship W4282942641A5015240577 @default.
• W4282942641 hasAuthorship W4282942641A5019059200 @default.
• W4282942641 hasAuthorship W4282942641A5033748161 @default.
• W4282942641 hasAuthorship W4282942641A5039041871 @default.
• W4282942641 hasAuthorship W4282942641A5073216193 @default.
• W4282942641 hasAuthorship W4282942641A5073734633 @default.
• W4282942641 hasAuthorship W4282942641A5079972363 @default.
• W4282942641 hasBestOaLocation W42829426411 @default.
• W4282942641 hasConcept C104317684 @default.
• W4282942641 hasConcept C132455925 @default.
• W4282942641 hasConcept C153911025 @default.
• W4282942641 hasConcept C22744801 @default.
• W4282942641 hasConcept C24586158 @default.
• W4282942641 hasConcept C54355233 @default.
• W4282942641 hasConcept C552990157 @default.
• W4282942641 hasConcept C86803240 @default.
• W4282942641 hasConcept C89684784 @default.
• W4282942641 hasConcept C98108389 @default.
• W4282942641 hasConceptScore W4282942641C104317684 @default.
• W4282942641 hasConceptScore W4282942641C132455925 @default.
• W4282942641 hasConceptScore W4282942641C153911025 @default.
• W4282942641 hasConceptScore W4282942641C22744801 @default.
• W4282942641 hasConceptScore W4282942641C24586158 @default.
• W4282942641 hasConceptScore W4282942641C54355233 @default.
• W4282942641 hasConceptScore W4282942641C552990157 @default.
• W4282942641 hasConceptScore W4282942641C86803240 @default.
• W4282942641 hasConceptScore W4282942641C89684784 @default.
• W4282942641 hasConceptScore W4282942641C98108389 @default.
• W4282942641 hasLocation W42829426411 @default.
• W4282942641 hasLocation W42829426412 @default.
• W4282942641 hasLocation W42829426413 @default.
• W4282942641 hasLocation W42829426414 @default.
• W4282942641 hasOpenAccess W4282942641 @default.

• next