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• W4282942783 abstract "Background. Oxidative stress (OS) and immune inflammation play complex intersections in the pathophysiology of ischemic stroke (IS). However, a competing endogenous RNA- (ceRNA-) based mechanism linked to the intersections in IS has not been explored. We aimed to identify potential OS-related signatures and analyze immune infiltration characteristics in IS. Methods. Three datasets (GSE22255, GSE110993, and GSE140275) from the GEO database were extracted. Differentially expressed long noncoding RNAs, microRNAs, and messenger RNAs (DElncRNAs, DEmiRNAs, and DEmRNAs) between IS patients and controls were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were explored. Moreover, a triple ceRNA network was constructed to reveal transcriptional regulation mechanisms. A comprehensive strategy among least absolute shrinkage and selection operator (LASSO) regression, DEmRNAs, uprelated DEmRNAs, and OS-related genes was adopted to select the best signature. Then, we evaluated and verified the discriminant ability of the signature via receiver operating characteristic (ROC) analysis. Immune infiltration characteristics were explored via the CIBERSORT algorithm. Moreover, the best signature was verified via qPCR and western blot methods in rat brain tissues and PC12 cells. Results. 11 DEmRNAs were identified totally. Enrichment analysis showed that the DEmRNAs were primarily concentrated in MAPK-associated biological processes and immune or inflammation-involved pathways. DUSP1 was identified as the best signature with an area under the ROC curve of 73.5% ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M1> <mn>95</mn> <mi>%</mi> <mtext>CI</mtext> <mo>=</mo> <mn>57.02</mn> </math> -89.98, <math xmlns=http://www.w3.org/1998/Math/MathML id=M2> <mtext>sensitivity</mtext> <mo>=</mo> <mn>95</mn> <mi>%</mi> </math> , and <math xmlns=http://www.w3.org/1998/Math/MathML id=M3> <mtext>specificity</mtext> <mo>=</mo> <mn>60</mn> <mi>%</mi> </math> ) in GSE22255 and 100.0% ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M4> <mn>95</mn> <mi>%</mi> <mtext>CI</mtext> <mo>=</mo> <mn>100.00</mn> </math> -100.00, <math xmlns=http://www.w3.org/1998/Math/MathML id=M5> <mtext>sensitivity</mtext> <mo>=</mo> <mn>100</mn> <mi>%</mi> </math> , and <math xmlns=http://www.w3.org/1998/Math/MathML id=M6> <mtext>specificity</mtext> <mo>=</mo> <mn>100</mn> <mi>%</mi> </math> ) in GSE140275. Importantly, we also identified the AC079305/DUSP1 axis in the ceRNA network. Immune infiltration showed that resting mast cells infiltrate less in IS patients compared with controls. And DUSP1 was negatively correlated with resting mast cells ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M7> <mi>r</mi> <mo>=</mo> <mo>−</mo> <mn>0.703</mn> </math> , <math xmlns=http://www.w3.org/1998/Math/MathML id=M8> <mi>P</mi> <mo><</mo> <mn>0.01</mn> </math> ), whereas it was positively correlated with neutrophils ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M9> <mi>r</mi> <mo>=</mo> <mn>0.339</mn> </math> , <math xmlns=http://www.w3.org/1998/Math/MathML id=M10> <mi>P</mi> <mo><</mo> <mn>0.05</mn> </math> ). Both in vivo and in vitro models confirmed the upregulated expression of DUSP1 and the downregulated expression of miR-429. Conclusion. This study identified the ceRNA-based AC079305/DUSP1 axis as a promising OS-related signature for IS. Immune infiltrating cells, especially mast cells, may exert a pivotal role in IS progression. Pharmacological agents targeting signatures, their receptors, or mast cells may shed a novel light on therapeutic targets for IS." @default.
• W4282942783 created "2022-06-16" @default.
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• W4282942783 date "2022-06-14" @default.
• W4282942783 modified "2023-10-14" @default.
• W4282942783 title "Investigating the AC079305/DUSP1 Axis as Oxidative Stress-Related Signatures and Immune Infiltration Characteristics in Ischemic Stroke" @default.
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• W4282942783 doi "https://doi.org/10.1155/2022/8432352" @default.
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