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- W4282946166 endingPage "e1010249" @default.
- W4282946166 startingPage "e1010249" @default.
- W4282946166 abstract "Molting is a widespread developmental process in which the external extracellular matrix (ECM), the cuticle, is remodeled to allow for organismal growth and environmental adaptation. Studies in the nematode Caenorhabditis elegans have identified a diverse set of molting-associated factors including signaling molecules, intracellular trafficking regulators, ECM components, and ECM-modifying enzymes such as matrix metalloproteases. C . elegans NEKL-2 and NEKL-3, two conserved members of the NEK family of protein kinases, are essential for molting and promote the endocytosis of environmental steroid-hormone precursors by the epidermis. Steroids in turn drive the cyclic induction of many genes required for molting. Here we report a role for the sole C . elegans ADAM–meltrin metalloprotease family member, ADM-2, as a mediator of molting. Loss of adm-2 , including mutations that disrupt the metalloprotease domain, led to the strong suppression of molting defects in partial loss-of-function nekl mutants. ADM-2 is expressed in the epidermis, and its trafficking through the endo-lysosomal network was disrupted after NEKL depletion. We identified the epidermally expressed low-density lipoprotein receptor–related protein, LRP-1, as a candidate target of ADM-2 regulation. Whereas loss of ADM-2 activity led to the upregulation of apical epidermal LRP-1, ADM-2 overexpression caused a reduction in LRP-1 levels. Consistent with this, several mammalian ADAMs, including the meltrin ADAM12, have been shown to regulate mammalian LRP1 via proteolysis. In contrast to mammalian homologs, however, the regulation of LRP-1 by ADM-2 does not appear to involve the metalloprotease function of ADM-2, nor is proteolytic processing of LRP-1 strongly affected in adm-2 mutants. Our findings suggest a noncanonical role for an ADAM family member in the regulation of a lipoprotein-like receptor and lead us to propose that endocytic trafficking may be important for both the internalization of factors that promote molting as well as the removal of proteins that can inhibit the process." @default.
- W4282946166 created "2022-06-16" @default.
- W4282946166 creator A5010795079 @default.
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- W4282946166 creator A5073102971 @default.
- W4282946166 creator A5083145430 @default.
- W4282946166 creator A5089455055 @default.
- W4282946166 date "2022-05-31" @default.
- W4282946166 modified "2023-10-18" @default.
- W4282946166 title "An unexpected role for the conserved ADAM-family metalloprotease ADM-2 in Caenorhabditis elegans molting" @default.
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