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• W4282946918 abstract "The use of mass spectrometry is currently widespread in polyphenol research because of its sensitivity and selectivity, but its usual high cost, reduced robustness, and nonavailability in many analytical laboratories considerably hinder its routine implementation. Herein, we describe the optimization and validation of a high-throughput, wide-coverage, and robust metabolomics method based on reversed-phase ultra-high-performance liquid chromatography with diode array detection for the identification and quantification of 69 phenolic compounds and related metabolites covering a broad chemical space of the characteristic secondary metabolome of plant foods. The method was satisfactorily validated following the Food and Drug Administration guidelines in terms of linearity (4-5 orders of magnitude), limits of quantification (0.007-3.6 mg L-1), matrix effect (60.5-124.4%), accuracy (63.4-126.7%), intraday precision (0.1-9.6%), interday precision (0.6-13.7%), specificity, and carryover. Then, it was successfully applied to characterize the phenolic fingerprints of diverse food products (i.e., olive oil, red wine, strawberry) and biological samples (i.e., urine), enabling not only the detection of many of the target compounds but also the semi-quantification of other phenolic metabolites tentatively identified based on their characteristic absorption spectra. Therefore, this method represents one step further toward time-efficient and low-cost polyphenol fingerprinting, with suitable applicability in the food industry to ensure food quality, safety, authenticity, and traceability." @default.
• W4282946918 created "2022-06-16" @default.
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• W4282946918 date "2022-06-15" @default.
• W4282946918 modified "2023-10-16" @default.
• W4282946918 title "High-Throughput Method for Wide-Coverage and Quantitative Phenolic Fingerprinting in Plant-Origin Foods and Urine Samples" @default.
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• W4282946918 doi "https://doi.org/10.1021/acs.jafc.2c01453" @default.
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