Matches in SemOpenAlex for { <https://semopenalex.org/work/W4282962183> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W4282962183 endingPage "52" @default.
- W4282962183 startingPage "45" @default.
- W4282962183 abstract "Carcinine is a natural imidazole-containing peptide derivative. It is widely used in the cosmetics industry as anti-aging supplement with antioxidant, anti-glycation and glycation reversal functions, and it also has a notable pharmacological effect as anti-tumor drug and in protection against retinopathy. However, a technological method for synthesis and production of carcinine has not been established. In this study, a whole-cell transformation system converting β-alanine and histamine to carcinine by the enzymes Ebony and phosphopantetheine transferase (Sfp) has been developed. The results revealed that the catalytic efficiency of the strain containing the fusion protein of Ebony and Sfp (Sfp-glycine-serine-glycine-Ebony, SGE) in Escherichia coli W3110 (WSGE strain) is significantly higher (7.45 mM) than the combinatorial strain of pET28a-ebony and pACYCDuet-sfp in E. coli BL21(DE3) (BSE strain) (2.17 mM). Under the optimal reaction conditions (25 ℃, pH 7.0, 12.5 g/L wet cells, 20 mM β-alanine and 40 mM histamine), the carcinine can be quickly synthesized within 24 h up to a concentration of 22.63 mM. To achieve a continuous and efficient conversion of the precursors, a batch-feeding catalysis was designed. With this system, β-alanine (40 mM) and histamine (40 mM) could be completely transformed to carcinine (40.34 mM) in 36 h with a productivity of 0.204 g/L h reaching a titer of 7.34 g/L. Hence, the batch-feeding whole-cell biocatalysis is a promising technology for the high yield production of carcinine which can promote the industrial production of carcinine." @default.
- W4282962183 created "2022-06-17" @default.
- W4282962183 creator A5035892129 @default.
- W4282962183 creator A5050774149 @default.
- W4282962183 creator A5057854558 @default.
- W4282962183 creator A5060341807 @default.
- W4282962183 creator A5065122687 @default.
- W4282962183 creator A5070021680 @default.
- W4282962183 creator A5071037763 @default.
- W4282962183 creator A5082160574 @default.
- W4282962183 date "2022-08-01" @default.
- W4282962183 modified "2023-10-18" @default.
- W4282962183 title "Whole-cell biotransformation for large scale production of carcinine in Escherichia coli" @default.
- W4282962183 cites W1972945472 @default.
- W4282962183 cites W2009421118 @default.
- W4282962183 cites W2016356492 @default.
- W4282962183 cites W2023791147 @default.
- W4282962183 cites W2024407964 @default.
- W4282962183 cites W2032493697 @default.
- W4282962183 cites W2033621140 @default.
- W4282962183 cites W2067053129 @default.
- W4282962183 cites W2071724458 @default.
- W4282962183 cites W2082874836 @default.
- W4282962183 cites W2085205963 @default.
- W4282962183 cites W2096581062 @default.
- W4282962183 cites W2098038332 @default.
- W4282962183 cites W2098931453 @default.
- W4282962183 cites W2101585764 @default.
- W4282962183 cites W2122500226 @default.
- W4282962183 cites W2126445561 @default.
- W4282962183 cites W2134341416 @default.
- W4282962183 cites W2144318457 @default.
- W4282962183 cites W2602946330 @default.
- W4282962183 cites W2912515037 @default.
- W4282962183 cites W4205090231 @default.
- W4282962183 doi "https://doi.org/10.1016/j.jbiotec.2022.06.003" @default.
- W4282962183 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35716886" @default.
- W4282962183 hasPublicationYear "2022" @default.
- W4282962183 type Work @default.
- W4282962183 citedByCount "1" @default.
- W4282962183 countsByYear W42829621832023 @default.
- W4282962183 crossrefType "journal-article" @default.
- W4282962183 hasAuthorship W4282962183A5035892129 @default.
- W4282962183 hasAuthorship W4282962183A5050774149 @default.
- W4282962183 hasAuthorship W4282962183A5057854558 @default.
- W4282962183 hasAuthorship W4282962183A5060341807 @default.
- W4282962183 hasAuthorship W4282962183A5065122687 @default.
- W4282962183 hasAuthorship W4282962183A5070021680 @default.
- W4282962183 hasAuthorship W4282962183A5071037763 @default.
- W4282962183 hasAuthorship W4282962183A5082160574 @default.
- W4282962183 hasConcept C104317684 @default.
- W4282962183 hasConcept C154881586 @default.
- W4282962183 hasConcept C161790260 @default.
- W4282962183 hasConcept C181199279 @default.
- W4282962183 hasConcept C185592680 @default.
- W4282962183 hasConcept C191503008 @default.
- W4282962183 hasConcept C27881333 @default.
- W4282962183 hasConcept C547475151 @default.
- W4282962183 hasConcept C55493867 @default.
- W4282962183 hasConceptScore W4282962183C104317684 @default.
- W4282962183 hasConceptScore W4282962183C154881586 @default.
- W4282962183 hasConceptScore W4282962183C161790260 @default.
- W4282962183 hasConceptScore W4282962183C181199279 @default.
- W4282962183 hasConceptScore W4282962183C185592680 @default.
- W4282962183 hasConceptScore W4282962183C191503008 @default.
- W4282962183 hasConceptScore W4282962183C27881333 @default.
- W4282962183 hasConceptScore W4282962183C547475151 @default.
- W4282962183 hasConceptScore W4282962183C55493867 @default.
- W4282962183 hasFunder F4320338464 @default.
- W4282962183 hasLocation W42829621831 @default.
- W4282962183 hasLocation W42829621832 @default.
- W4282962183 hasOpenAccess W4282962183 @default.
- W4282962183 hasPrimaryLocation W42829621831 @default.
- W4282962183 hasRelatedWork W1494880142 @default.
- W4282962183 hasRelatedWork W1974318975 @default.
- W4282962183 hasRelatedWork W1978861814 @default.
- W4282962183 hasRelatedWork W2108221116 @default.
- W4282962183 hasRelatedWork W2373828514 @default.
- W4282962183 hasRelatedWork W2944302360 @default.
- W4282962183 hasRelatedWork W3080279548 @default.
- W4282962183 hasRelatedWork W3164511160 @default.
- W4282962183 hasRelatedWork W4285133860 @default.
- W4282962183 hasRelatedWork W4372236107 @default.
- W4282962183 hasVolume "354" @default.
- W4282962183 isParatext "false" @default.
- W4282962183 isRetracted "false" @default.
- W4282962183 workType "article" @default.