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- W4282983721 abstract "Abstract Bacillus subtilis can metabolize a wide array of sugars under aerobic conditions, including hexoses via the EMP pathway and some pentoses via the pentose phosphate pathway, and secretes amylases, cellulases and hemicellulases. However, studies using different sugars under anaerobic conditions are scarce. B. subtilis can also grow anaerobically by using a terminal electron acceptor or by fermentation metabolizing glucose to lactic acid (LA), butanediol, and other products under non-aerated conditions. B. subtilis ER382 (∆ alsS ), with the 2,3-butanediol pathway inactivated, was obtained from strain 168 trp + . Such deletion allowed obtaining a homo l-lactogenic strain with increased LA production. B. subtilis ER382 was evaluated in batch cultures with 10 g/L of seven different carbon sources under non-aerated conditions and rich media. B. subtilis ER382 metabolized glucose, fructose, cellobiose, sucrose, and arabinose under non-aerated conditions and produced LA as the only fermentation product. However, strains 168 trp + and ER382 were unable to ferment and grow on glycerol or starch under the tested conditions. The higher biomass yield and faster rates of sugar consumption (1.01 g SUGAR /L h) and L-LA production (1.32 g LA /L h) were obtained with glucose as the carbon source and the strain containing the alsS deletion. The L-LA yield on consumed sugars was above the theoretical because carbon sources from yeast extract and amino acids from casein hydrolysate were also used for LA synthesis." @default.
- W4282983721 created "2022-06-17" @default.
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- W4282983721 date "2022-06-16" @default.
- W4282983721 modified "2023-10-01" @default.
- W4282983721 title "Fermentation performance of Bacillus subtilis wild type and alsS- mutant using different carbon sources" @default.
- W4282983721 doi "https://doi.org/10.21203/rs.3.rs-1746482/v1" @default.
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