Matches in SemOpenAlex for { <https://semopenalex.org/work/W4283022300> ?p ?o ?g. }
- W4283022300 abstract "Abstract Spectrally diverse fluorescent proteins (FPs) provide straightforward means for multiplexed imaging of biological systems. Among FPs fitting standard color channels, blue FPs (BFPs) are characterized by lower brightness compared to other spectral counterparts. Furthermore, available BFPs were not systematically characterized for imaging in cultured mammalian cells and common model organisms. Here we introduce a pair of new BFPs, named Electra1 and Electra2, developed through hierarchical screening in bacterial and mammalian cells using a novel dual-expression vector. We performed systematic benchmarking of Electras against state-of-art BFPs in cultured mammalian cells and demonstrated their utility as fluorescent tags for structural proteins. The Electras variants were validated for multicolor neuroimaging in Caenorhabditis elegans , zebrafish larvae, and mice in comparison with one of the best in the class BFP mTagBFP2 using one-photon and two-photon microscopy. The developed BFPs are suitable for multicolor imaging of cultured cells and model organisms in vivo. We believe that the described dual-expression vector has a great potential to be adopted by protein engineers for directed molecular evolution of FPs." @default.
- W4283022300 created "2022-06-18" @default.
- W4283022300 creator A5003224266 @default.
- W4283022300 creator A5017852247 @default.
- W4283022300 creator A5019892128 @default.
- W4283022300 creator A5020589001 @default.
- W4283022300 creator A5032185006 @default.
- W4283022300 creator A5050975943 @default.
- W4283022300 creator A5056496295 @default.
- W4283022300 creator A5061354710 @default.
- W4283022300 creator A5069268661 @default.
- W4283022300 creator A5071201906 @default.
- W4283022300 creator A5082083173 @default.
- W4283022300 creator A5085689718 @default.
- W4283022300 date "2022-06-17" @default.
- W4283022300 modified "2023-10-14" @default.
- W4283022300 title "Dual-expression system for blue fluorescent protein optimization" @default.
- W4283022300 cites W1525527328 @default.
- W4283022300 cites W1592923459 @default.
- W4283022300 cites W1828400526 @default.
- W4283022300 cites W185922212 @default.
- W4283022300 cites W1932712520 @default.
- W4283022300 cites W1944127002 @default.
- W4283022300 cites W1970550565 @default.
- W4283022300 cites W1981841369 @default.
- W4283022300 cites W1982864032 @default.
- W4283022300 cites W2007402269 @default.
- W4283022300 cites W2007981920 @default.
- W4283022300 cites W2017831287 @default.
- W4283022300 cites W2017989927 @default.
- W4283022300 cites W2030383182 @default.
- W4283022300 cites W2039756922 @default.
- W4283022300 cites W2040361016 @default.
- W4283022300 cites W2043272632 @default.
- W4283022300 cites W2053679951 @default.
- W4283022300 cites W2075701819 @default.
- W4283022300 cites W2100365580 @default.
- W4283022300 cites W2115734610 @default.
- W4283022300 cites W2116469093 @default.
- W4283022300 cites W2120305868 @default.
- W4283022300 cites W2121951679 @default.
- W4283022300 cites W2122881260 @default.
- W4283022300 cites W2123924700 @default.
- W4283022300 cites W2128018926 @default.
- W4283022300 cites W2132892857 @default.
- W4283022300 cites W2139414608 @default.
- W4283022300 cites W2140706307 @default.
- W4283022300 cites W2143094150 @default.
- W4283022300 cites W2150392611 @default.
- W4283022300 cites W2160919229 @default.
- W4283022300 cites W2163024465 @default.
- W4283022300 cites W2415046030 @default.
- W4283022300 cites W2416471421 @default.
- W4283022300 cites W2471526055 @default.
- W4283022300 cites W2516071618 @default.
- W4283022300 cites W2773372730 @default.
- W4283022300 cites W2792403811 @default.
- W4283022300 cites W2802260035 @default.
- W4283022300 cites W2921718105 @default.
- W4283022300 cites W2946216085 @default.
- W4283022300 cites W2949294922 @default.
- W4283022300 cites W2951372251 @default.
- W4283022300 cites W2951421438 @default.
- W4283022300 cites W2959524261 @default.
- W4283022300 cites W2999147930 @default.
- W4283022300 cites W3000358775 @default.
- W4283022300 cites W3100686222 @default.
- W4283022300 cites W3131882878 @default.
- W4283022300 cites W3214868139 @default.
- W4283022300 cites W4205809079 @default.
- W4283022300 cites W4212971126 @default.
- W4283022300 cites W4220853974 @default.
- W4283022300 cites W805509078 @default.
- W4283022300 doi "https://doi.org/10.1038/s41598-022-13214-0" @default.
- W4283022300 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35715437" @default.
- W4283022300 hasPublicationYear "2022" @default.
- W4283022300 type Work @default.
- W4283022300 citedByCount "12" @default.
- W4283022300 countsByYear W42830223002022 @default.
- W4283022300 countsByYear W42830223002023 @default.
- W4283022300 crossrefType "journal-article" @default.
- W4283022300 hasAuthorship W4283022300A5003224266 @default.
- W4283022300 hasAuthorship W4283022300A5017852247 @default.
- W4283022300 hasAuthorship W4283022300A5019892128 @default.
- W4283022300 hasAuthorship W4283022300A5020589001 @default.
- W4283022300 hasAuthorship W4283022300A5032185006 @default.
- W4283022300 hasAuthorship W4283022300A5050975943 @default.
- W4283022300 hasAuthorship W4283022300A5056496295 @default.
- W4283022300 hasAuthorship W4283022300A5061354710 @default.
- W4283022300 hasAuthorship W4283022300A5069268661 @default.
- W4283022300 hasAuthorship W4283022300A5071201906 @default.
- W4283022300 hasAuthorship W4283022300A5082083173 @default.
- W4283022300 hasAuthorship W4283022300A5085689718 @default.
- W4283022300 hasBestOaLocation W42830223001 @default.
- W4283022300 hasConcept C104317684 @default.
- W4283022300 hasConcept C120665830 @default.
- W4283022300 hasConcept C121332964 @default.
- W4283022300 hasConcept C123894998 @default.
- W4283022300 hasConcept C142613039 @default.
- W4283022300 hasConcept C156904656 @default.