Matches in SemOpenAlex for { <https://semopenalex.org/work/W4283025982> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W4283025982 endingPage "106519" @default.
- W4283025982 startingPage "106519" @default.
- W4283025982 abstract "Clostridium perfringens is a Gram-positive pathogen with low GC content. To identify genes that are transcribed at higher levels when the bacteria grow on a surface, we used RNA-seq in a previous study to measure global transcript levels of cells grown in three types of media on both plates and in liquid culture. We found the arcABDC-argR operon is induced >1000-fold when the cells were grown on plates than in liquid brain heart infusion (BHI). In addition, the pyrBICFZDE operon was transcribed >1000-fold higher in liquid BHI than on plates. Biochemical analysis of C. perfringens proteins is usually accomplished by cloning and expressing the relevant genes in Escherichia coli, a Gram-negative bacterium. Here we utilize both the arcA and pyrB promoters to express and purify proteins from C. perfringens plate and liquid-grown cultures, respectively. Three mg of the His-tagged cytoplasmic protein PilM were obtained when the pilM gene was expressed in cells grown on 10 BHI plates using the arcA promoter. Using the pyrB promoter, 0.85 mg of the C. perfringens His-tagged secreted toxin collagenase was purified from the culture supernatant of 500 ml of cells grown in liquid BHI. In the process of constructing clones, we found we can transform C. perfringens strain HN13 directly with DNA from an in vitro ligation mix, bypassing E. coli. These environmentally regulated promoters can be used to express clostridial or other low GC content genes for protein purification without the addition of an inducer molecule." @default.
- W4283025982 created "2022-06-18" @default.
- W4283025982 creator A5013597460 @default.
- W4283025982 creator A5065407514 @default.
- W4283025982 creator A5068178888 @default.
- W4283025982 date "2022-08-01" @default.
- W4283025982 modified "2023-10-18" @default.
- W4283025982 title "Mining transcriptome data: Utilization of environmentally regulated promoters for protein expression and purification in Clostridium perfringens" @default.
- W4283025982 cites W1842268384 @default.
- W4283025982 cites W1872405824 @default.
- W4283025982 cites W1937790690 @default.
- W4283025982 cites W1951346612 @default.
- W4283025982 cites W2016974786 @default.
- W4283025982 cites W2020541351 @default.
- W4283025982 cites W2071826103 @default.
- W4283025982 cites W2080440175 @default.
- W4283025982 cites W2100429002 @default.
- W4283025982 cites W2124070339 @default.
- W4283025982 cites W2151391555 @default.
- W4283025982 cites W2157804398 @default.
- W4283025982 cites W2158611246 @default.
- W4283025982 cites W2159410845 @default.
- W4283025982 cites W2163258602 @default.
- W4283025982 cites W2267115418 @default.
- W4283025982 cites W2297307165 @default.
- W4283025982 cites W2314274831 @default.
- W4283025982 cites W2341874138 @default.
- W4283025982 cites W2410505453 @default.
- W4283025982 cites W2467190568 @default.
- W4283025982 cites W2587397254 @default.
- W4283025982 cites W2594194353 @default.
- W4283025982 cites W2786618088 @default.
- W4283025982 cites W3002677589 @default.
- W4283025982 doi "https://doi.org/10.1016/j.mimet.2022.106519" @default.
- W4283025982 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35718279" @default.
- W4283025982 hasPublicationYear "2022" @default.
- W4283025982 type Work @default.
- W4283025982 citedByCount "0" @default.
- W4283025982 crossrefType "journal-article" @default.
- W4283025982 hasAuthorship W4283025982A5013597460 @default.
- W4283025982 hasAuthorship W4283025982A5065407514 @default.
- W4283025982 hasAuthorship W4283025982A5068178888 @default.
- W4283025982 hasConcept C101762097 @default.
- W4283025982 hasConcept C104317684 @default.
- W4283025982 hasConcept C150194340 @default.
- W4283025982 hasConcept C153911025 @default.
- W4283025982 hasConcept C162317418 @default.
- W4283025982 hasConcept C203075996 @default.
- W4283025982 hasConcept C2778660310 @default.
- W4283025982 hasConcept C2779050283 @default.
- W4283025982 hasConcept C2779116991 @default.
- W4283025982 hasConcept C523546767 @default.
- W4283025982 hasConcept C54355233 @default.
- W4283025982 hasConcept C547475151 @default.
- W4283025982 hasConcept C55493867 @default.
- W4283025982 hasConcept C86803240 @default.
- W4283025982 hasConcept C89423630 @default.
- W4283025982 hasConceptScore W4283025982C101762097 @default.
- W4283025982 hasConceptScore W4283025982C104317684 @default.
- W4283025982 hasConceptScore W4283025982C150194340 @default.
- W4283025982 hasConceptScore W4283025982C153911025 @default.
- W4283025982 hasConceptScore W4283025982C162317418 @default.
- W4283025982 hasConceptScore W4283025982C203075996 @default.
- W4283025982 hasConceptScore W4283025982C2778660310 @default.
- W4283025982 hasConceptScore W4283025982C2779050283 @default.
- W4283025982 hasConceptScore W4283025982C2779116991 @default.
- W4283025982 hasConceptScore W4283025982C523546767 @default.
- W4283025982 hasConceptScore W4283025982C54355233 @default.
- W4283025982 hasConceptScore W4283025982C547475151 @default.
- W4283025982 hasConceptScore W4283025982C55493867 @default.
- W4283025982 hasConceptScore W4283025982C86803240 @default.
- W4283025982 hasConceptScore W4283025982C89423630 @default.
- W4283025982 hasFunder F4320306080 @default.
- W4283025982 hasFunder F4320332161 @default.
- W4283025982 hasFunder F4320337355 @default.
- W4283025982 hasLocation W42830259821 @default.
- W4283025982 hasLocation W42830259822 @default.
- W4283025982 hasOpenAccess W4283025982 @default.
- W4283025982 hasPrimaryLocation W42830259821 @default.
- W4283025982 hasRelatedWork W1488640901 @default.
- W4283025982 hasRelatedWork W1590969345 @default.
- W4283025982 hasRelatedWork W1634139602 @default.
- W4283025982 hasRelatedWork W2153122502 @default.
- W4283025982 hasRelatedWork W2171277769 @default.
- W4283025982 hasRelatedWork W2417450025 @default.
- W4283025982 hasRelatedWork W2443274608 @default.
- W4283025982 hasRelatedWork W2751262199 @default.
- W4283025982 hasRelatedWork W3108873220 @default.
- W4283025982 hasRelatedWork W4283025982 @default.
- W4283025982 hasVolume "199" @default.
- W4283025982 isParatext "false" @default.
- W4283025982 isRetracted "false" @default.
- W4283025982 workType "article" @default.