Matches in SemOpenAlex for { <https://semopenalex.org/work/W4283031270> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W4283031270 abstract "Abstract Background Bacterial outer membrane proteins (Omps) play crucial roles in environmental senses, stress responses and substance transportation. Our previous study has found that OmpA contributes to planktonic growth, biocide resistance, biofilm formation and swimming motility in Citrobacter werkmanii , whereas molecular functions of OmpF in this strain remain largely unknown. Hence, in this work, the ompF was firstly knocked out from the genome of C. werkmanii using a homologous recombination method and its phenotypical alternations of ∆ompF were then thoroughly characterized with the parental wild type (WT) and complementary ( ∆ompF -com) strains using biochemical and molecular approaches. Results The results demonstrated that the swimming ability of ∆ompF was reduced on semi-solid plates compared to WT due to down-regulation of flgC , flgH , fliK and fliF . Meanwhile, the deletion of ompF leads to a decrease of biofilm formation on both glass and polystyrene surfaces because of the reduced cell aggregation. Additionally, the inactivation of ompF also induced different osmotic stress (carbon sources and mental ions) responses of its biofilms comparing with that of WT and ∆ompF -com. Finally, a total of 6 maltose metabolic genes of lamB , malE , malK , malG , malM and malF were all up-regulated in ∆ompF . Gene knockout and HPLC results further revealed that MalEFGK2 cluster mainly contributed to maltose transportation in C. werkmanii . What’s more, for the first time, we have found that the upstream promoter of OmpF and its transcription can be combined with and regulated by MalT. Conclusions Overall, OmpF contributes to a variety of biochemical processes and molecular functions in C. werkmanii and even may be acted as a targeted site to restrain biofilm formation." @default.
- W4283031270 created "2022-06-18" @default.
- W4283031270 creator A5008838534 @default.
- W4283031270 creator A5009201642 @default.
- W4283031270 creator A5032393123 @default.
- W4283031270 creator A5061505296 @default.
- W4283031270 creator A5067810311 @default.
- W4283031270 creator A5068670108 @default.
- W4283031270 creator A5076525992 @default.
- W4283031270 creator A5081159152 @default.
- W4283031270 creator A5085611398 @default.
- W4283031270 creator A5091025324 @default.
- W4283031270 date "2022-06-16" @default.
- W4283031270 modified "2023-09-27" @default.
- W4283031270 title "Outer membrane protein of OmpF contributes to swimming motility, biofilm formation, osmotic response as well as the transcription of maltose metabolic genes in Citrobacter werkmanii" @default.
- W4283031270 doi "https://doi.org/10.21203/rs.3.rs-1367552/v1" @default.
- W4283031270 hasPublicationYear "2022" @default.
- W4283031270 type Work @default.
- W4283031270 citedByCount "0" @default.
- W4283031270 crossrefType "posted-content" @default.
- W4283031270 hasAuthorship W4283031270A5008838534 @default.
- W4283031270 hasAuthorship W4283031270A5009201642 @default.
- W4283031270 hasAuthorship W4283031270A5032393123 @default.
- W4283031270 hasAuthorship W4283031270A5061505296 @default.
- W4283031270 hasAuthorship W4283031270A5067810311 @default.
- W4283031270 hasAuthorship W4283031270A5068670108 @default.
- W4283031270 hasAuthorship W4283031270A5076525992 @default.
- W4283031270 hasAuthorship W4283031270A5081159152 @default.
- W4283031270 hasAuthorship W4283031270A5085611398 @default.
- W4283031270 hasAuthorship W4283031270A5091025324 @default.
- W4283031270 hasBestOaLocation W42830312701 @default.
- W4283031270 hasConcept C104317684 @default.
- W4283031270 hasConcept C138885662 @default.
- W4283031270 hasConcept C146587185 @default.
- W4283031270 hasConcept C179926584 @default.
- W4283031270 hasConcept C2777317804 @default.
- W4283031270 hasConcept C41895202 @default.
- W4283031270 hasConcept C523546767 @default.
- W4283031270 hasConcept C54355233 @default.
- W4283031270 hasConcept C547475151 @default.
- W4283031270 hasConcept C55493867 @default.
- W4283031270 hasConcept C58123911 @default.
- W4283031270 hasConcept C58207958 @default.
- W4283031270 hasConcept C86339819 @default.
- W4283031270 hasConcept C86803240 @default.
- W4283031270 hasConcept C95444343 @default.
- W4283031270 hasConceptScore W4283031270C104317684 @default.
- W4283031270 hasConceptScore W4283031270C138885662 @default.
- W4283031270 hasConceptScore W4283031270C146587185 @default.
- W4283031270 hasConceptScore W4283031270C179926584 @default.
- W4283031270 hasConceptScore W4283031270C2777317804 @default.
- W4283031270 hasConceptScore W4283031270C41895202 @default.
- W4283031270 hasConceptScore W4283031270C523546767 @default.
- W4283031270 hasConceptScore W4283031270C54355233 @default.
- W4283031270 hasConceptScore W4283031270C547475151 @default.
- W4283031270 hasConceptScore W4283031270C55493867 @default.
- W4283031270 hasConceptScore W4283031270C58123911 @default.
- W4283031270 hasConceptScore W4283031270C58207958 @default.
- W4283031270 hasConceptScore W4283031270C86339819 @default.
- W4283031270 hasConceptScore W4283031270C86803240 @default.
- W4283031270 hasConceptScore W4283031270C95444343 @default.
- W4283031270 hasLocation W42830312701 @default.
- W4283031270 hasOpenAccess W4283031270 @default.
- W4283031270 hasPrimaryLocation W42830312701 @default.
- W4283031270 hasRelatedWork W1755317122 @default.
- W4283031270 hasRelatedWork W1993189984 @default.
- W4283031270 hasRelatedWork W2029158435 @default.
- W4283031270 hasRelatedWork W2093750194 @default.
- W4283031270 hasRelatedWork W2099868116 @default.
- W4283031270 hasRelatedWork W2153910890 @default.
- W4283031270 hasRelatedWork W2283457788 @default.
- W4283031270 hasRelatedWork W3015788176 @default.
- W4283031270 hasRelatedWork W4323052102 @default.
- W4283031270 hasRelatedWork W2187902633 @default.
- W4283031270 isParatext "false" @default.
- W4283031270 isRetracted "false" @default.
- W4283031270 workType "article" @default.