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- W4283165997 abstract "Reverse transcriptases (RTs) are RNA-dependent DNA polymerases able of synthesizing DNA (complementary DNA or cDNA) from an RNA template. RTs are especially useful in RNA-based nucleic acid detection techniques. Due to its high catalytic activity and fidelity, one of the most widely used RTs in diagnostics and molecular biology is the RT from the Moloney Murine Leukemia Virus (MMLV). However, RT-MMLV is thermally unstable, so previous studies have produced a RT variant called RT-MM4 carrying mutations of positive charges in four amino acids (E286R/E302K/L435R/D524A). This protocol describes the optimized expression process, as well as the FPLC purification of RT-MM4 for use in isothermal amplification techniques, such as end-point colorimetric or real-time fluorometric RT-LAMP." @default.
- W4283165997 created "2022-06-21" @default.
- W4283165997 creator A5061269094 @default.
- W4283165997 date "2022-06-08" @default.
- W4283165997 modified "2023-09-28" @default.
- W4283165997 title "Expression and purification of recombinant MM4 reverse transcriptase (RT) v1" @default.
- W4283165997 cites W2043074543 @default.
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- W4283165997 doi "https://doi.org/10.17504/protocols.io.8epv59objg1b/v1" @default.
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