Matches in SemOpenAlex for { <https://semopenalex.org/work/W4283170071> ?p ?o ?g. }
- W4283170071 abstract "Abstract In situ imaging of biomolecular location with nanoscale resolution enables mapping of the building blocks of life throughout biological systems in normal and disease states. Expansion microscopy (ExM), by physically enlarging specimens in an isotropic fashion, enables nanoimaging on standard light microscopes. Key to ExM is the equipping of different kinds of molecule, with different kinds of anchoring moiety, so they can all be pulled apart by polymer swelling. Here we present a multifunctional anchor, an acrylate epoxide, that enables multiple kinds of molecules ( e.g., proteins and RNAs) to be equipped with anchors in a single experimental step. This reagent simplifies ExM protocols and greatly reduces cost (by 2-10 fold for a typical multiplexed ExM experiment) compared to previous strategies for equipping RNAs with anchors. We show that this unified ExM (uniExM) protocol can be used to preserve and visualize RNA transcripts, proteins in biologically relevant ultrastructure, and sets of RNA transcripts in patient-derived xenograft (PDX) cancer tissues, and can support the visualization of other kinds of biomolecular species as well. Thus, uniExM may find many uses in the simple, multimodal nanoscale analysis of cells and tissues." @default.
- W4283170071 created "2022-06-21" @default.
- W4283170071 creator A5002767537 @default.
- W4283170071 creator A5007930912 @default.
- W4283170071 creator A5018465623 @default.
- W4283170071 creator A5022170125 @default.
- W4283170071 creator A5039142645 @default.
- W4283170071 creator A5049693793 @default.
- W4283170071 creator A5075795118 @default.
- W4283170071 creator A5086953418 @default.
- W4283170071 creator A5087640504 @default.
- W4283170071 creator A5088141052 @default.
- W4283170071 date "2022-06-19" @default.
- W4283170071 modified "2023-09-27" @default.
- W4283170071 title "A Multifunctional Anchor for Multimodal Expansion Microscopy" @default.
- W4283170071 cites W1976299634 @default.
- W4283170071 cites W2003460765 @default.
- W4283170071 cites W2052975116 @default.
- W4283170071 cites W2065490663 @default.
- W4283170071 cites W2095072830 @default.
- W4283170071 cites W2102628698 @default.
- W4283170071 cites W2317883772 @default.
- W4283170071 cites W2349604136 @default.
- W4283170071 cites W2406725268 @default.
- W4283170071 cites W2408470505 @default.
- W4283170071 cites W2460281481 @default.
- W4283170071 cites W2472582738 @default.
- W4283170071 cites W2494923364 @default.
- W4283170071 cites W2735146241 @default.
- W4283170071 cites W2752919824 @default.
- W4283170071 cites W2764205300 @default.
- W4283170071 cites W2769501787 @default.
- W4283170071 cites W2885194328 @default.
- W4283170071 cites W2902929870 @default.
- W4283170071 cites W2904533595 @default.
- W4283170071 cites W2905344744 @default.
- W4283170071 cites W2912791793 @default.
- W4283170071 cites W2915415778 @default.
- W4283170071 cites W2940982798 @default.
- W4283170071 cites W2982921542 @default.
- W4283170071 cites W3007870821 @default.
- W4283170071 cites W3021302753 @default.
- W4283170071 cites W3025798397 @default.
- W4283170071 cites W3032120775 @default.
- W4283170071 cites W3032982274 @default.
- W4283170071 cites W3046519579 @default.
- W4283170071 cites W3082225631 @default.
- W4283170071 cites W3112506189 @default.
- W4283170071 cites W3147908094 @default.
- W4283170071 cites W3178364274 @default.
- W4283170071 cites W4200574769 @default.
- W4283170071 cites W4214499193 @default.
- W4283170071 cites W4225876567 @default.
- W4283170071 cites W4232515155 @default.
- W4283170071 doi "https://doi.org/10.1101/2022.06.19.496699" @default.
- W4283170071 hasPublicationYear "2022" @default.
- W4283170071 type Work @default.
- W4283170071 citedByCount "1" @default.
- W4283170071 countsByYear W42831700712023 @default.
- W4283170071 crossrefType "posted-content" @default.
- W4283170071 hasAuthorship W4283170071A5002767537 @default.
- W4283170071 hasAuthorship W4283170071A5007930912 @default.
- W4283170071 hasAuthorship W4283170071A5018465623 @default.
- W4283170071 hasAuthorship W4283170071A5022170125 @default.
- W4283170071 hasAuthorship W4283170071A5039142645 @default.
- W4283170071 hasAuthorship W4283170071A5049693793 @default.
- W4283170071 hasAuthorship W4283170071A5075795118 @default.
- W4283170071 hasAuthorship W4283170071A5086953418 @default.
- W4283170071 hasAuthorship W4283170071A5087640504 @default.
- W4283170071 hasAuthorship W4283170071A5088141052 @default.
- W4283170071 hasBestOaLocation W42831700711 @default.
- W4283170071 hasConcept C104317684 @default.
- W4283170071 hasConcept C120665830 @default.
- W4283170071 hasConcept C121332964 @default.
- W4283170071 hasConcept C138268822 @default.
- W4283170071 hasConcept C147080431 @default.
- W4283170071 hasConcept C154945302 @default.
- W4283170071 hasConcept C171250308 @default.
- W4283170071 hasConcept C185592680 @default.
- W4283170071 hasConcept C192562407 @default.
- W4283170071 hasConcept C36464697 @default.
- W4283170071 hasConcept C41008148 @default.
- W4283170071 hasConcept C49853544 @default.
- W4283170071 hasConcept C55493867 @default.
- W4283170071 hasConcept C67705224 @default.
- W4283170071 hasConcept C70721500 @default.
- W4283170071 hasConcept C86803240 @default.
- W4283170071 hasConceptScore W4283170071C104317684 @default.
- W4283170071 hasConceptScore W4283170071C120665830 @default.
- W4283170071 hasConceptScore W4283170071C121332964 @default.
- W4283170071 hasConceptScore W4283170071C138268822 @default.
- W4283170071 hasConceptScore W4283170071C147080431 @default.
- W4283170071 hasConceptScore W4283170071C154945302 @default.
- W4283170071 hasConceptScore W4283170071C171250308 @default.
- W4283170071 hasConceptScore W4283170071C185592680 @default.
- W4283170071 hasConceptScore W4283170071C192562407 @default.
- W4283170071 hasConceptScore W4283170071C36464697 @default.
- W4283170071 hasConceptScore W4283170071C41008148 @default.
- W4283170071 hasConceptScore W4283170071C49853544 @default.
- W4283170071 hasConceptScore W4283170071C55493867 @default.