Matches in SemOpenAlex for { <https://semopenalex.org/work/W4283276328> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W4283276328 abstract "Abstract Most fast synaptic inhibitions in the mammalian brain are mediated by GABA A receptors (GABA A Rs). An appropriate level of GABA A R expression at the cell surface is essential for neurodevelopment and the efficacy of GABAergic synaptic transmission. We previously reported that brefeldin A-inhibited GDP/GTP exchange factor 1 (BIG1), a binding partner of GABA A Rs, plays an important role in trafficking GABA A Rs to the cell surface. However, its regulatory mechanisms remain unknown. In the present study, we identified a new cellular protein, 14-3-3ζ, which can interact with the β subunit of GABA A Rs and BIG1 both in vitro and in vivo and colocalizes in the soma, dendrites and axons of hippocampal neurons. Overexpression of 14-3-3ζ-WT increased the surface expression of BIG1 in dendrites and axons, as well as the binding of BIG1 with GABA A R. Depleted 14-3-3ζ with efficacious siRNA attenuated the interaction between BIG1 and GABA A Rs and resulted in significant decreases in the surface expression levels of BIG1 and GABA A R. GABA A R agonist treatment increased the expression levels of BIG1 and 14-3-3ζ on the surface, indicating that 14-3-3ζ is involved in regulating BIG1-mediated GABA A R surface expression. Depletion of BIG1 or 14-3-3ζ significantly decreased GABA A R expression at the cell surface and suppressed the GABA-gated influx of chloride ions. These data indicate that the combination of 14-3-3ζ and BIG1 is required for GABA A R membrane expression. Our results provide a potential promising therapeutic target for neurological disorders involving GABAergic synaptic transmission." @default.
- W4283276328 created "2022-06-23" @default.
- W4283276328 creator A5003630787 @default.
- W4283276328 creator A5011614759 @default.
- W4283276328 creator A5029334739 @default.
- W4283276328 creator A5054358807 @default.
- W4283276328 creator A5067887572 @default.
- W4283276328 creator A5086839135 @default.
- W4283276328 date "2022-06-21" @default.
- W4283276328 modified "2023-10-16" @default.
- W4283276328 title "14-3-3ζ mediates GABAAR activation by interacting with BIG1" @default.
- W4283276328 doi "https://doi.org/10.21203/rs.3.rs-1706977/v1" @default.
- W4283276328 hasPublicationYear "2022" @default.
- W4283276328 type Work @default.
- W4283276328 citedByCount "0" @default.
- W4283276328 crossrefType "posted-content" @default.
- W4283276328 hasAuthorship W4283276328A5003630787 @default.
- W4283276328 hasAuthorship W4283276328A5011614759 @default.
- W4283276328 hasAuthorship W4283276328A5029334739 @default.
- W4283276328 hasAuthorship W4283276328A5054358807 @default.
- W4283276328 hasAuthorship W4283276328A5067887572 @default.
- W4283276328 hasAuthorship W4283276328A5086839135 @default.
- W4283276328 hasBestOaLocation W42832763281 @default.
- W4283276328 hasConcept C119062480 @default.
- W4283276328 hasConcept C158617107 @default.
- W4283276328 hasConcept C168258287 @default.
- W4283276328 hasConcept C169760540 @default.
- W4283276328 hasConcept C170493617 @default.
- W4283276328 hasConcept C17077164 @default.
- W4283276328 hasConcept C185592680 @default.
- W4283276328 hasConcept C200170125 @default.
- W4283276328 hasConcept C2779177108 @default.
- W4283276328 hasConcept C2781373781 @default.
- W4283276328 hasConcept C55493867 @default.
- W4283276328 hasConcept C86803240 @default.
- W4283276328 hasConcept C95444343 @default.
- W4283276328 hasConceptScore W4283276328C119062480 @default.
- W4283276328 hasConceptScore W4283276328C158617107 @default.
- W4283276328 hasConceptScore W4283276328C168258287 @default.
- W4283276328 hasConceptScore W4283276328C169760540 @default.
- W4283276328 hasConceptScore W4283276328C170493617 @default.
- W4283276328 hasConceptScore W4283276328C17077164 @default.
- W4283276328 hasConceptScore W4283276328C185592680 @default.
- W4283276328 hasConceptScore W4283276328C200170125 @default.
- W4283276328 hasConceptScore W4283276328C2779177108 @default.
- W4283276328 hasConceptScore W4283276328C2781373781 @default.
- W4283276328 hasConceptScore W4283276328C55493867 @default.
- W4283276328 hasConceptScore W4283276328C86803240 @default.
- W4283276328 hasConceptScore W4283276328C95444343 @default.
- W4283276328 hasLocation W42832763281 @default.
- W4283276328 hasOpenAccess W4283276328 @default.
- W4283276328 hasPrimaryLocation W42832763281 @default.
- W4283276328 hasRelatedWork W1964470295 @default.
- W4283276328 hasRelatedWork W1980598704 @default.
- W4283276328 hasRelatedWork W2000043887 @default.
- W4283276328 hasRelatedWork W2035827812 @default.
- W4283276328 hasRelatedWork W2046987765 @default.
- W4283276328 hasRelatedWork W2104834189 @default.
- W4283276328 hasRelatedWork W2137591095 @default.
- W4283276328 hasRelatedWork W2220090227 @default.
- W4283276328 hasRelatedWork W2561322497 @default.
- W4283276328 hasRelatedWork W2772626215 @default.
- W4283276328 isParatext "false" @default.
- W4283276328 isRetracted "false" @default.
- W4283276328 workType "article" @default.