FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4283276533> ?p ?o ?g. }

• W4283276533 endingPage "6886" @default.
• W4283276533 startingPage "6886" @default.
• W4283276533 abstract "Positron emission tomography is the imaging modality of choice when it comes to the high sensitivity detection of key markers of thrombosis and inflammation, such as activated platelets. We, previously, generated a fluorine-18 labelled single-chain antibody (scFv) against ligand-induced binding sites (LIBS) on activated platelets, binding it to the highly abundant platelet glycoprotein integrin receptor IIb/IIIa. We used a non-site-specific bio conjugation approach with N-succinimidyl-4-[18F]fluorobenzoate (S[18F]FB), leading to a mixture of products with reduced antigen binding. In the present study, we have developed and characterised a novel fluorine-18 PET radiotracer, based on this antibody, using site-specific bio conjugation to engineer cysteine residues with N-[2-(4-[18F]fluorobenzamido)ethyl]maleimide ([18F]FBEM). ScFvanti-LIBS and control antibody mut-scFv, with engineered C-terminal cysteine, were reduced, and then, they reacted with N-[2-(4-[18F]fluorobenzamido)ethyl]maleimide ([18F]FBEM). Radiolabelled scFv was injected into mice with FeCl3-induced thrombus in the left carotid artery. Clots were imaged in a PET MR imaging system, and the amount of radioactivity in major organs was measured using an ionisation chamber and image analysis. Assessment of vessel injury, as well as the biodistribution of the radiolabelled scFv, was studied. In the in vivo experiments, we found uptake of the targeted tracer in the injured vessel, compared with the non-injured vessel, as well as a high uptake of both tracers in the kidney, lung, and muscle. As expected, both tracers cleared rapidly via the kidney. Surprisingly, a large quantity of both tracers was taken up by organs with a high glutathione content, such as the muscle and lung, due to the instability of the maleimide cysteine bond in vivo, which warrants further investigations. This limits the ability of the novel antibody radiotracer 18F-scFvanti-LIBS to bind to the target in vivo and, therefore, as a useful agent for the sensitive detection of activated platelets. We describe the first fluorine-18 variant of the scFvanti-LIBS against activated platelets using site-specific bio conjugation." @default.
• W4283276533 created "2022-06-23" @default.
• W4283276533 creator A5007968984 @default.
• W4283276533 creator A5008496267 @default.
• W4283276533 creator A5022261595 @default.
• W4283276533 creator A5025399748 @default.
• W4283276533 creator A5045654706 @default.
• W4283276533 creator A5049936296 @default.
• W4283276533 creator A5055670455 @default.
• W4283276533 creator A5061272373 @default.
• W4283276533 creator A5062913670 @default.
• W4283276533 creator A5068289720 @default.
• W4283276533 creator A5078312966 @default.
• W4283276533 creator A5087721166 @default.
• W4283276533 date "2022-06-21" @default.
• W4283276533 modified "2023-10-18" @default.
• W4283276533 title "18F Site-Specific Labelling of a Single-Chain Antibody against Activated Platelets for the Detection of Acute Thrombosis in Positron Emission Tomography" @default.
• W4283276533 cites W1555361166 @default.
• W4283276533 cites W1802550271 @default.
• W4283276533 cites W1829208371 @default.
• W4283276533 cites W1965962082 @default.
• W4283276533 cites W1974291199 @default.
• W4283276533 cites W1975288666 @default.
• W4283276533 cites W1977241343 @default.
• W4283276533 cites W1982803287 @default.
• W4283276533 cites W1991463599 @default.
• W4283276533 cites W1992175481 @default.
• W4283276533 cites W1996732617 @default.
• W4283276533 cites W1998493057 @default.
• W4283276533 cites W2005185823 @default.
• W4283276533 cites W2011209568 @default.
• W4283276533 cites W2014327275 @default.
• W4283276533 cites W2023034663 @default.
• W4283276533 cites W2023511690 @default.
• W4283276533 cites W2035774412 @default.
• W4283276533 cites W2039193236 @default.
• W4283276533 cites W2041469065 @default.
• W4283276533 cites W2046185660 @default.
• W4283276533 cites W2061136178 @default.
• W4283276533 cites W2069888156 @default.
• W4283276533 cites W2073272324 @default.
• W4283276533 cites W2080726207 @default.
• W4283276533 cites W2084141349 @default.
• W4283276533 cites W2086409927 @default.
• W4283276533 cites W2091492240 @default.
• W4283276533 cites W2094941678 @default.
• W4283276533 cites W2098686036 @default.
• W4283276533 cites W2115063969 @default.
• W4283276533 cites W2120439472 @default.
• W4283276533 cites W2138566208 @default.
• W4283276533 cites W2147243248 @default.
• W4283276533 cites W2148706200 @default.
• W4283276533 cites W2151247869 @default.
• W4283276533 cites W2154410581 @default.
• W4283276533 cites W2160512390 @default.
• W4283276533 cites W2166750469 @default.
• W4283276533 cites W2170426247 @default.
• W4283276533 cites W2194814336 @default.
• W4283276533 cites W2292833094 @default.
• W4283276533 cites W2322836921 @default.
• W4283276533 cites W2344977044 @default.
• W4283276533 cites W2508090059 @default.
• W4283276533 cites W2525471267 @default.
• W4283276533 cites W2579273479 @default.
• W4283276533 cites W2605923435 @default.
• W4283276533 cites W2606382560 @default.
• W4283276533 cites W2788414803 @default.
• W4283276533 cites W36424441 @default.
• W4283276533 doi "https://doi.org/10.3390/ijms23136886" @default.
• W4283276533 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35805892" @default.
• W4283276533 hasPublicationYear "2022" @default.
• W4283276533 type Work @default.
• W4283276533 citedByCount "0" @default.
• W4283276533 crossrefType "journal-article" @default.
• W4283276533 hasAuthorship W4283276533A5007968984 @default.
• W4283276533 hasAuthorship W4283276533A5008496267 @default.
• W4283276533 hasAuthorship W4283276533A5022261595 @default.
• W4283276533 hasAuthorship W4283276533A5025399748 @default.
• W4283276533 hasAuthorship W4283276533A5045654706 @default.
• W4283276533 hasAuthorship W4283276533A5049936296 @default.
• W4283276533 hasAuthorship W4283276533A5055670455 @default.
• W4283276533 hasAuthorship W4283276533A5061272373 @default.
• W4283276533 hasAuthorship W4283276533A5062913670 @default.
• W4283276533 hasAuthorship W4283276533A5068289720 @default.
• W4283276533 hasAuthorship W4283276533A5078312966 @default.
• W4283276533 hasAuthorship W4283276533A5087721166 @default.
• W4283276533 hasBestOaLocation W42832765331 @default.
• W4283276533 hasConcept C126322002 @default.
• W4283276533 hasConcept C136339569 @default.
• W4283276533 hasConcept C150903083 @default.
• W4283276533 hasConcept C153911025 @default.
• W4283276533 hasConcept C185592680 @default.
• W4283276533 hasConcept C202751555 @default.
• W4283276533 hasConcept C203014093 @default.
• W4283276533 hasConcept C207001950 @default.
• W4283276533 hasConcept C2775842073 @default.
• W4283276533 hasConcept C2776970183 @default.
• W4283276533 hasConcept C2777807558 @default.

• next