Matches in SemOpenAlex for { <https://semopenalex.org/work/W4283278570> ?p ?o ?g. }
- W4283278570 abstract "Abstract In vitro systems mimicking brain regions, brain organoids, are revolutionizing the neuroscience field. However, characterization of their electrical activity has remained a challenge as this requires readout at millisecond timescale in 3D at single-neuron resolution. While custom-built microscopes used with genetically encoded sensors are now opening this door, a full 3D characterization of organoid neural activity has not been performed yet, limited by the combined complexity of the optical and the biological system. Here, we introduce an accessible minimalistic light-sheet microscope to the neuroscience community. Designed as an add-on to a standard inverted microscope it can be assembled within one day. In contrast to existing simplistic setups, our platform is suited to record volumetric calcium traces. We successfully extracted 4D calcium traces at high temporal resolution by using a lightweight piezo stage to allow for 5Hz volumetric scanning combined with a processing pipeline for true 3D neuronal trace segmentation. As a proof of principle, we created a 3D connectivity map of a stem cell derived neuron spheroid by imaging its activity. Our fast, low complexity setup empowers researchers to study the formation of neuronal networks in vitro for fundamental and neurodegeneration research." @default.
- W4283278570 created "2022-06-23" @default.
- W4283278570 creator A5012251732 @default.
- W4283278570 creator A5017134903 @default.
- W4283278570 creator A5059728059 @default.
- W4283278570 creator A5066295423 @default.
- W4283278570 creator A5070089309 @default.
- W4283278570 creator A5080151790 @default.
- W4283278570 date "2022-06-21" @default.
- W4283278570 modified "2023-10-06" @default.
- W4283278570 title "A minimal-complexity light-sheet microscope maps network activity in 3D neuronal systems" @default.
- W4283278570 cites W1553983967 @default.
- W4283278570 cites W1894928878 @default.
- W4283278570 cites W1964549255 @default.
- W4283278570 cites W1971912541 @default.
- W4283278570 cites W1986408320 @default.
- W4283278570 cites W1987145582 @default.
- W4283278570 cites W1993355218 @default.
- W4283278570 cites W1994574914 @default.
- W4283278570 cites W1995206354 @default.
- W4283278570 cites W2018286352 @default.
- W4283278570 cites W2036505824 @default.
- W4283278570 cites W2040215769 @default.
- W4283278570 cites W2041844713 @default.
- W4283278570 cites W2046020742 @default.
- W4283278570 cites W2051685988 @default.
- W4283278570 cites W2057132085 @default.
- W4283278570 cites W2062382534 @default.
- W4283278570 cites W2062619493 @default.
- W4283278570 cites W2069306062 @default.
- W4283278570 cites W2089315051 @default.
- W4283278570 cites W2099616257 @default.
- W4283278570 cites W2110021835 @default.
- W4283278570 cites W2113927646 @default.
- W4283278570 cites W2147918634 @default.
- W4283278570 cites W2160799121 @default.
- W4283278570 cites W2160919229 @default.
- W4283278570 cites W2165682071 @default.
- W4283278570 cites W2187074433 @default.
- W4283278570 cites W2222248410 @default.
- W4283278570 cites W2315788237 @default.
- W4283278570 cites W2319627288 @default.
- W4283278570 cites W2397643408 @default.
- W4283278570 cites W2466296023 @default.
- W4283278570 cites W2469581795 @default.
- W4283278570 cites W2545370334 @default.
- W4283278570 cites W2604794514 @default.
- W4283278570 cites W2609844460 @default.
- W4283278570 cites W2614623901 @default.
- W4283278570 cites W2732612903 @default.
- W4283278570 cites W2741799782 @default.
- W4283278570 cites W2753168648 @default.
- W4283278570 cites W2754889070 @default.
- W4283278570 cites W2766567783 @default.
- W4283278570 cites W2805271873 @default.
- W4283278570 cites W2882986564 @default.
- W4283278570 cites W2887477202 @default.
- W4283278570 cites W2919730323 @default.
- W4283278570 cites W2940115943 @default.
- W4283278570 cites W2944813712 @default.
- W4283278570 cites W2949410666 @default.
- W4283278570 cites W2950569309 @default.
- W4283278570 cites W2956078734 @default.
- W4283278570 cites W2963621587 @default.
- W4283278570 cites W2970454553 @default.
- W4283278570 cites W2976039826 @default.
- W4283278570 cites W2977688599 @default.
- W4283278570 cites W3003422837 @default.
- W4283278570 cites W3087102910 @default.
- W4283278570 cites W3118034245 @default.
- W4283278570 cites W3119945494 @default.
- W4283278570 cites W3155807332 @default.
- W4283278570 cites W3158678351 @default.
- W4283278570 cites W3158688284 @default.
- W4283278570 cites W3193479663 @default.
- W4283278570 cites W3199161326 @default.
- W4283278570 cites W3199689835 @default.
- W4283278570 cites W3214371609 @default.
- W4283278570 cites W4210778691 @default.
- W4283278570 cites W4281672588 @default.
- W4283278570 cites W4288535621 @default.
- W4283278570 doi "https://doi.org/10.1101/2022.06.20.496852" @default.
- W4283278570 hasPublicationYear "2022" @default.
- W4283278570 type Work @default.
- W4283278570 citedByCount "0" @default.
- W4283278570 crossrefType "posted-content" @default.
- W4283278570 hasAuthorship W4283278570A5012251732 @default.
- W4283278570 hasAuthorship W4283278570A5017134903 @default.
- W4283278570 hasAuthorship W4283278570A5059728059 @default.
- W4283278570 hasAuthorship W4283278570A5066295423 @default.
- W4283278570 hasAuthorship W4283278570A5070089309 @default.
- W4283278570 hasAuthorship W4283278570A5080151790 @default.
- W4283278570 hasBestOaLocation W42832785701 @default.
- W4283278570 hasConcept C113976765 @default.
- W4283278570 hasConcept C120665830 @default.
- W4283278570 hasConcept C121332964 @default.
- W4283278570 hasConcept C147080431 @default.
- W4283278570 hasConcept C154945302 @default.
- W4283278570 hasConcept C169760540 @default.
- W4283278570 hasConcept C171250308 @default.