FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4283383631> ?p ?o ?g. }

• W4283383631 endingPage "2073" @default.
• W4283383631 startingPage "2072" @default.
• W4283383631 abstract "Background: To study the proliferative and differentiating ability of hematopoietic precursors and to determine the hypoplastic variant of neutropenia, a cultural method is used. A special semi-liquid nutrient medium based on methylcellulose is used in this study. A certain amount of nucleated bone marrow cells is incubated for a fixed period. Colony forming units (CFU) make colonies in depth and on the surface of the medium. The number of colonies is proportional to the amount of the corresponding colony-forming cells in cell suspension. The colonies of each hematopoietic cell lines have characteristic morphological features which make it possible to distinguish and count them. Aims: Evaluating the colony-forming ability of the bone marrow using a culture method as part of the differential diagnosis of neutropenia of unknown origin. Methods: The colony-forming ability of the bone marrow was studied in 20 patients of children’s department of hematology clinic, surveyed in 2016-2021 (13 boys, 7 girls). Age ranged from 1.2 to 17 (median 13) years. The criterion for the neutropenia was the absolute number of neutrophils less than 1.5 thousand in 1 μl of peripheral blood. For cultivation, a nutrient medium based on methylcellulose (MethoCult Human No. 4434, StemCell Technologies, Canada) was used, inoculation was performed according to the standard method. Cultures are incubated at 37°C in 5% CO2.The proliferative potential and differentiation ability of bone marrow cells was determined by counting the number of corresponding colonies in a 14-day culture. The norm was considered for granulocyte-erythrocyte-macrophage-megakaryocytic colonies (CFU-GEMM) - 0-8 per 105 myelokaryocytes, granulocytic (CFU-G) - 7-35, macrophage (CFU-M) - 1-18, eosinophilic (CFU-Eo) – 0-20, respectively. The total number of colonies is normally 49-166. Results: The total number of colonies ranged from 6 to 158 (median 55). Of these, within the reference values - 55.0% of patients. The number of CFU-G was determined in the range from 1 to 45 (median - 4), in a number of patients the granulocytic cell line of the bone marrow was not weakened: in 25.0% of cases, a normal number of CFU-G was found, in 10.0% - increased. The number of CFU-M ranged from 3 to 67 (median 21.5); an increased number of macrophage precursors was observed in 60.0% of neutropenic patients. A direct correlation between the number of CFU-G and CFU-M was determined, Spearman’s coefficient (r) 0.64 (p=0,003). The increased activity of the granulocytic cell line of hematopoiesis was accompanied by a high number of CFU-M. In all patients, the number of CFU-GEMM (0-1, median - 0) and CFU-Eo (0-10, median - 1.5) was determined within the normal range. Summary/Conclusion: Neutropenia is a group of conditions with different etiology and pathogenesis. The culture method can be used for the differential diagnosis of neutropenia of unknown origin. Correlation of the number of granulocytic and macrophage precursors in the bone marrow in patients with neutropenia can be due to active hematopoiesis maybe because of cytokine stimulation." @default.
• W4283383631 created "2022-06-25" @default.
• W4283383631 creator A5006098543 @default.
• W4283383631 creator A5007678893 @default.
• W4283383631 creator A5038770189 @default.
• W4283383631 creator A5066729955 @default.
• W4283383631 creator A5081168797 @default.
• W4283383631 date "2022-06-01" @default.
• W4283383631 modified "2023-09-26" @default.
• W4283383631 title "PB2202: FEATURES OF COLONY FORMING ABILITY OF BONE MARROW IN PATIENTS WITH NEUTROPENIA" @default.
• W4283383631 doi "https://doi.org/10.1097/01.hs9.0000851636.90192.3a" @default.
• W4283383631 hasPublicationYear "2022" @default.
• W4283383631 type Work @default.
• W4283383631 citedByCount "0" @default.
• W4283383631 crossrefType "journal-article" @default.
• W4283383631 hasAuthorship W4283383631A5006098543 @default.
• W4283383631 hasAuthorship W4283383631A5007678893 @default.
• W4283383631 hasAuthorship W4283383631A5038770189 @default.
• W4283383631 hasAuthorship W4283383631A5066729955 @default.
• W4283383631 hasAuthorship W4283383631A5081168797 @default.
• W4283383631 hasBestOaLocation W42833836311 @default.
• W4283383631 hasConcept C109159458 @default.
• W4283383631 hasConcept C126322002 @default.
• W4283383631 hasConcept C142724271 @default.
• W4283383631 hasConcept C16685009 @default.
• W4283383631 hasConcept C192285135 @default.
• W4283383631 hasConcept C203014093 @default.
• W4283383631 hasConcept C2776694085 @default.
• W4283383631 hasConcept C2777063308 @default.
• W4283383631 hasConcept C2780007613 @default.
• W4283383631 hasConcept C28328180 @default.
• W4283383631 hasConcept C2992880124 @default.
• W4283383631 hasConcept C3017910909 @default.
• W4283383631 hasConcept C523546767 @default.
• W4283383631 hasConcept C54355233 @default.
• W4283383631 hasConcept C59822182 @default.
• W4283383631 hasConcept C71924100 @default.
• W4283383631 hasConcept C86803240 @default.
• W4283383631 hasConcept C95444343 @default.
• W4283383631 hasConceptScore W4283383631C109159458 @default.
• W4283383631 hasConceptScore W4283383631C126322002 @default.
• W4283383631 hasConceptScore W4283383631C142724271 @default.
• W4283383631 hasConceptScore W4283383631C16685009 @default.
• W4283383631 hasConceptScore W4283383631C192285135 @default.
• W4283383631 hasConceptScore W4283383631C203014093 @default.
• W4283383631 hasConceptScore W4283383631C2776694085 @default.
• W4283383631 hasConceptScore W4283383631C2777063308 @default.
• W4283383631 hasConceptScore W4283383631C2780007613 @default.
• W4283383631 hasConceptScore W4283383631C28328180 @default.
• W4283383631 hasConceptScore W4283383631C2992880124 @default.
• W4283383631 hasConceptScore W4283383631C3017910909 @default.
• W4283383631 hasConceptScore W4283383631C523546767 @default.
• W4283383631 hasConceptScore W4283383631C54355233 @default.
• W4283383631 hasConceptScore W4283383631C59822182 @default.
• W4283383631 hasConceptScore W4283383631C71924100 @default.
• W4283383631 hasConceptScore W4283383631C86803240 @default.
• W4283383631 hasConceptScore W4283383631C95444343 @default.
• W4283383631 hasLocation W42833836311 @default.
• W4283383631 hasLocation W42833836312 @default.
• W4283383631 hasLocation W42833836313 @default.
• W4283383631 hasOpenAccess W4283383631 @default.
• W4283383631 hasPrimaryLocation W42833836311 @default.
• W4283383631 hasRelatedWork W1480825656 @default.
• W4283383631 hasRelatedWork W1964962741 @default.
• W4283383631 hasRelatedWork W1975791409 @default.
• W4283383631 hasRelatedWork W1997413330 @default.
• W4283383631 hasRelatedWork W2332158939 @default.
• W4283383631 hasRelatedWork W2371320166 @default.
• W4283383631 hasRelatedWork W2402336525 @default.
• W4283383631 hasRelatedWork W2414394217 @default.
• W4283383631 hasRelatedWork W2992684550 @default.
• W4283383631 hasRelatedWork W4364856070 @default.
• W4283383631 hasVolume "6" @default.
• W4283383631 isParatext "false" @default.
• W4283383631 isRetracted "false" @default.
• W4283383631 workType "article" @default.