Matches in SemOpenAlex for { <https://semopenalex.org/work/W4283400512> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W4283400512 endingPage "pdb.top099606" @default.
- W4283400512 startingPage "pdb.top099606" @default.
- W4283400512 abstract "When labeled antibodies are used to detect antigens in cells or tissues, several characteristics of an antigen can be readily determined. Most importantly, cell staining will show both the presence and subcellular localization of an antigen. Double-labeling techniques permit the simultaneous detection of two antigens, allowing comparisons of the relative distribution of different antigens. Many cell-staining methods can also be used in conjunction with conventional histological stains and autoradiographic methods to compare the localization of the antigen with other markers. Cell staining can also be used in pathology studies for determining such variables as the type of infectious organism, the progenitor of a neoplastic cell, or the presence of an inflammatory response. With certain modifications, the procedure can be used to purify cells on the basis of the antigenic composition of their cell surface. Cell staining is a versatile technique and, if the antigen is highly localized, can detect as few as a thousand antigen molecules in a cell or tissue. In some circumstances, cell staining may also be used to determine the approximate concentration of an antigen. Improvements in antibody labeling methods, microscopes, cameras, and image analyzers are rapidly extending the sensitivity of cell-staining procedures and are making these techniques more quantitative. Even without these improvements, cell staining can yield important qualitative and semiquantitative data. This introduction describes protocols for cell staining techniques and includes a discussion of major constraints, antibody selection, and troubleshooting." @default.
- W4283400512 created "2022-06-25" @default.
- W4283400512 creator A5058975989 @default.
- W4283400512 date "2022-06-01" @default.
- W4283400512 modified "2023-09-29" @default.
- W4283400512 title "Cell Staining" @default.
- W4283400512 cites W1040634928 @default.
- W4283400512 cites W205008539 @default.
- W4283400512 cites W2111039706 @default.
- W4283400512 cites W2152125010 @default.
- W4283400512 cites W2211589810 @default.
- W4283400512 cites W2930877031 @default.
- W4283400512 cites W2933986364 @default.
- W4283400512 cites W2934238484 @default.
- W4283400512 cites W2943775480 @default.
- W4283400512 cites W2954717796 @default.
- W4283400512 cites W2954874057 @default.
- W4283400512 cites W3023295352 @default.
- W4283400512 cites W3046998433 @default.
- W4283400512 cites W3047003133 @default.
- W4283400512 cites W3047506533 @default.
- W4283400512 cites W3107249439 @default.
- W4283400512 cites W3107552843 @default.
- W4283400512 cites W3133567028 @default.
- W4283400512 cites W3135153793 @default.
- W4283400512 cites W3135508279 @default.
- W4283400512 cites W3164018377 @default.
- W4283400512 cites W3164203810 @default.
- W4283400512 cites W4239136149 @default.
- W4283400512 cites W4245238610 @default.
- W4283400512 cites W4283372176 @default.
- W4283400512 cites W46230381 @default.
- W4283400512 doi "https://doi.org/10.1101/pdb.top099606" @default.
- W4283400512 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35750473" @default.
- W4283400512 hasPublicationYear "2022" @default.
- W4283400512 type Work @default.
- W4283400512 citedByCount "1" @default.
- W4283400512 countsByYear W42834005122022 @default.
- W4283400512 crossrefType "journal-article" @default.
- W4283400512 hasAuthorship W4283400512A5058975989 @default.
- W4283400512 hasConcept C147483822 @default.
- W4283400512 hasConcept C1491633281 @default.
- W4283400512 hasConcept C153911025 @default.
- W4283400512 hasConcept C159654299 @default.
- W4283400512 hasConcept C203014093 @default.
- W4283400512 hasConcept C54355233 @default.
- W4283400512 hasConcept C55493867 @default.
- W4283400512 hasConcept C74864618 @default.
- W4283400512 hasConcept C86803240 @default.
- W4283400512 hasConceptScore W4283400512C147483822 @default.
- W4283400512 hasConceptScore W4283400512C1491633281 @default.
- W4283400512 hasConceptScore W4283400512C153911025 @default.
- W4283400512 hasConceptScore W4283400512C159654299 @default.
- W4283400512 hasConceptScore W4283400512C203014093 @default.
- W4283400512 hasConceptScore W4283400512C54355233 @default.
- W4283400512 hasConceptScore W4283400512C55493867 @default.
- W4283400512 hasConceptScore W4283400512C74864618 @default.
- W4283400512 hasConceptScore W4283400512C86803240 @default.
- W4283400512 hasIssue "6" @default.
- W4283400512 hasLocation W42834005121 @default.
- W4283400512 hasLocation W42834005122 @default.
- W4283400512 hasOpenAccess W4283400512 @default.
- W4283400512 hasPrimaryLocation W42834005121 @default.
- W4283400512 hasRelatedWork W19727122 @default.
- W4283400512 hasRelatedWork W20004777 @default.
- W4283400512 hasRelatedWork W23161797 @default.
- W4283400512 hasRelatedWork W30199205 @default.
- W4283400512 hasRelatedWork W31383154 @default.
- W4283400512 hasRelatedWork W31730231 @default.
- W4283400512 hasRelatedWork W37043633 @default.
- W4283400512 hasRelatedWork W38814086 @default.
- W4283400512 hasRelatedWork W39077762 @default.
- W4283400512 hasRelatedWork W33644022 @default.
- W4283400512 hasVolume "2022" @default.
- W4283400512 isParatext "false" @default.
- W4283400512 isRetracted "false" @default.
- W4283400512 workType "article" @default.