Matches in SemOpenAlex for { <https://semopenalex.org/work/W4283526649> ?p ?o ?g. }
- W4283526649 abstract "Abstract Folding of nascent chains emerging from the ribosome is a challenge in cellular protein homeostasis, which in eukaryotes is met by an Hsp70 chaperone triad directly binding at the ribosomal tunnel exit. The conserved ribosome-associated complex (RAC) consists of the non-canonical Hsp70 Ssz1 and the J-domain protein Zuotin (Zuo1), which in fungi acts together with the canonical Hsp70 protein Ssb. Here, we determined high-resolution cryo-electron microscopy structures of RAC bound to the 80S ribosome. RAC adopts two distinct conformations accommodating continuous ribosomal rotation by a flexible lever arm. The heterodimer is held together by a tight interaction between the Ssz1 substrate-binding domain (SBD) and the N-terminus of Zuo1, with additional contacts between the Ssz1 nucleotide-binding domain (NBD) and the Zuo1 J- and ZHD domains that form a rigid unit. The Zuo1 HPD-motif conserved in J-proteins is masked by the Ssz1 NBD, different from the canonical Hsp70 J-protein contact, however, allowing to position Ssb for activation by Zuo1. Our data provide the basis for understanding how RAC cooperates with Ssb at the ribosome in dynamic nascent chain interaction and protein folding." @default.
- W4283526649 created "2022-06-27" @default.
- W4283526649 creator A5013788995 @default.
- W4283526649 creator A5021763568 @default.
- W4283526649 creator A5027553849 @default.
- W4283526649 creator A5053602762 @default.
- W4283526649 creator A5057468179 @default.
- W4283526649 creator A5060010220 @default.
- W4283526649 creator A5090859550 @default.
- W4283526649 date "2022-06-24" @default.
- W4283526649 modified "2023-09-27" @default.
- W4283526649 title "Structural inventory of cotranslational protein folding by the eukaryotic RAC complex" @default.
- W4283526649 cites W1964273724 @default.
- W4283526649 cites W1965791457 @default.
- W4283526649 cites W1967960279 @default.
- W4283526649 cites W1972121275 @default.
- W4283526649 cites W1980505864 @default.
- W4283526649 cites W1981873223 @default.
- W4283526649 cites W1986087947 @default.
- W4283526649 cites W1987224264 @default.
- W4283526649 cites W1995112257 @default.
- W4283526649 cites W2001289286 @default.
- W4283526649 cites W2005369928 @default.
- W4283526649 cites W2023111823 @default.
- W4283526649 cites W2046756800 @default.
- W4283526649 cites W2055269320 @default.
- W4283526649 cites W2060578508 @default.
- W4283526649 cites W2064292796 @default.
- W4283526649 cites W2064878094 @default.
- W4283526649 cites W2070020117 @default.
- W4283526649 cites W2086812659 @default.
- W4283526649 cites W2091593275 @default.
- W4283526649 cites W2102886264 @default.
- W4283526649 cites W2104234755 @default.
- W4283526649 cites W2124026197 @default.
- W4283526649 cites W2129919915 @default.
- W4283526649 cites W2132629607 @default.
- W4283526649 cites W2180229411 @default.
- W4283526649 cites W2296137721 @default.
- W4283526649 cites W2409252905 @default.
- W4283526649 cites W2473711714 @default.
- W4283526649 cites W2526312394 @default.
- W4283526649 cites W2549314952 @default.
- W4283526649 cites W2559912926 @default.
- W4283526649 cites W2571495805 @default.
- W4283526649 cites W2593511418 @default.
- W4283526649 cites W2595886211 @default.
- W4283526649 cites W2736142726 @default.
- W4283526649 cites W2745179958 @default.
- W4283526649 cites W2765322245 @default.
- W4283526649 cites W2780354290 @default.
- W4283526649 cites W2791354025 @default.
- W4283526649 cites W2901399587 @default.
- W4283526649 cites W2902272528 @default.
- W4283526649 cites W2945028502 @default.
- W4283526649 cites W2953320394 @default.
- W4283526649 cites W2954926994 @default.
- W4283526649 cites W3011827202 @default.
- W4283526649 cites W3083406432 @default.
- W4283526649 cites W3131760520 @default.
- W4283526649 cites W3202814848 @default.
- W4283526649 cites W4210541422 @default.
- W4283526649 doi "https://doi.org/10.1101/2022.06.24.497458" @default.
- W4283526649 hasPublicationYear "2022" @default.
- W4283526649 type Work @default.
- W4283526649 citedByCount "0" @default.
- W4283526649 crossrefType "posted-content" @default.
- W4283526649 hasAuthorship W4283526649A5013788995 @default.
- W4283526649 hasAuthorship W4283526649A5021763568 @default.
- W4283526649 hasAuthorship W4283526649A5027553849 @default.
- W4283526649 hasAuthorship W4283526649A5053602762 @default.
- W4283526649 hasAuthorship W4283526649A5057468179 @default.
- W4283526649 hasAuthorship W4283526649A5060010220 @default.
- W4283526649 hasAuthorship W4283526649A5090859550 @default.
- W4283526649 hasBestOaLocation W42835266491 @default.
- W4283526649 hasConcept C104317684 @default.
- W4283526649 hasConcept C113027372 @default.
- W4283526649 hasConcept C12554922 @default.
- W4283526649 hasConcept C142724271 @default.
- W4283526649 hasConcept C185592680 @default.
- W4283526649 hasConcept C204328495 @default.
- W4283526649 hasConcept C2775962898 @default.
- W4283526649 hasConcept C38062823 @default.
- W4283526649 hasConcept C512185932 @default.
- W4283526649 hasConcept C55493867 @default.
- W4283526649 hasConcept C67705224 @default.
- W4283526649 hasConcept C71924100 @default.
- W4283526649 hasConcept C86803240 @default.
- W4283526649 hasConcept C88478588 @default.
- W4283526649 hasConcept C95444343 @default.
- W4283526649 hasConceptScore W4283526649C104317684 @default.
- W4283526649 hasConceptScore W4283526649C113027372 @default.
- W4283526649 hasConceptScore W4283526649C12554922 @default.
- W4283526649 hasConceptScore W4283526649C142724271 @default.
- W4283526649 hasConceptScore W4283526649C185592680 @default.
- W4283526649 hasConceptScore W4283526649C204328495 @default.
- W4283526649 hasConceptScore W4283526649C2775962898 @default.
- W4283526649 hasConceptScore W4283526649C38062823 @default.
- W4283526649 hasConceptScore W4283526649C512185932 @default.
- W4283526649 hasConceptScore W4283526649C55493867 @default.