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• W4283589419 abstract "Abstract The human pathogen Yersinia enterocolitica strain W22703 is characterized by its toxicity towards invertebrates that requires the insecticidal toxin complex (Tc) proteins encoded by the pathogenicity island Tc-PAI Ye . Molecular and pathophysiological details of insect larvae infection and killing by this pathogen, however, have not been dissected. Here, we applied oral infection of Galleria mellonella (Greater wax moth) larvae to study the colonisation, proliferation, tissue invasion, and killing activity of W22703. We demonstrated that this strain is strongly toxic towards the larvae, in which they proliferate by more than three orders of magnitude within six days post infection. Deletion mutants of genes tcaA and tccC were atoxic for the insect. W22703 ΔtccC, in contrast to W22703 Δ tcaA, initially proliferated before being eliminated from the host, thus confirming TcaA and TccC as membrane-binding Tc subunit and ADP-ribosylating enzyme, respectively. Time course experiments revealed a Tc-dependent infection process starting with midgut colonisation that is followed by invasion of the hemolymph where the pathogen elicits morphological changes of hemocytes and strongly proliferates. The in vivo transcriptome of strain W22703 shows that the pathogen undergoes a drastic reprogramming of central cell functions and gains access to numerous carbohydrate and amino acid resources within the insect. Strikingly, a mutant lacking a phage-related holin/endolysin (HE) cassette, which is located within Tc-PAI Ye , resembled the phenotypes of W22703 Δ tcaA, suggesting that this dual lysis cassette is an example for a phage-related function that has been adapted for the release of a bacterial toxin." @default.
• W4283589419 created "2022-06-28" @default.
• W4283589419 creator A5046665812 @default.
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• W4283589419 date "2022-06-27" @default.
• W4283589419 modified "2023-09-28" @default.
• W4283589419 title "Dissecting the invasion of <i>Galleria mellonella</i> by <i>Yersinia enterocolitica</i> reveals metabolic adaptations and a role of a phage lysis cassette in insect killing" @default.
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• W4283589419 doi "https://doi.org/10.1101/2022.06.27.497489" @default.
• W4283589419 hasPublicationYear "2022" @default.
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