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- W4283697788 abstract "Hyaluronan modulates tumour progression, including cell adhesion, cohesion, proliferation and invasion, and the cancer stem cell phenotype. In ovarian cancer, high levels of stromal hyaluronan are associated with poor prognosis. In this work, hyaluronan synthases (HAS1-3) and hyaluronidases (HYAL1-4, PH-20, HYALP1) were examined with regard to different levels of gene expression and its influence on ovarian cancer patients' survival. The impact of a siRNA depletion of HAS2 was investigated in vitro.Using the Kaplan-Meier Plotter tool, we investigated the influence of hyaluronic synthases and hyaluronidases on the survival of a collective of 1435 ovarian cancer patients. Differences in gene expression between normal (n = 46) and cancerous (n = 744) ovarian tissue were examined using the TNMplot database. Following an evaluation of hyaluronan-related gene expression in the ATCC ovarian cancer panel, we studied SKOV3 and SW 626 ovarian cancer cells subjected to HAS2 siRNA or control siRNA treatment in terms of HAS1-3, HYAL2 and HYAL3 mRNA expression. We investigated the ability to form spheroids using the Hanging Drop method and the response to chemotherapy at different concentrations using the MTT Assay. By STRING analysis, interactions within the enzymes of the hyaluronic acid system and with binding partners were visualized.HAS1, HYAL1 and HYAL4 mRNA expression is significantly upregulated, whereas HAS2, HYAL2 and HYAL3 mRNA expression is significantly downregulated in ovarian cancer tissue compared to controls. HAS2 improves cell viability, the capability to form tumour spheroids and has a negative prognostic value regarding overall survival. Lower HAS2 expression and high expression of HYAL2 and HYAL3 favours the survival of ovarian cancer patients. HAS2 knockdown cells and control cells showed a moderate response to combinatorial in vitro chemotherapy with taxol and cisplatin.In conclusion, our study shows that the hyaluronic acid system has a relevant influence on the survival of ovarian cancer patients and could therefore be considered as a possible prognostic factor." @default.
- W4283697788 created "2022-06-30" @default.
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- W4283697788 date "2022-06-29" @default.
- W4283697788 modified "2023-10-16" @default.
- W4283697788 title "The hyaluronan-related genes HAS2, HYAL1-4, PH20 and HYALP1 are associated with prognosis, cell viability and spheroid formation capacity in ovarian cancer" @default.
- W4283697788 cites W1218084201 @default.
- W4283697788 cites W1949898671 @default.
- W4283697788 cites W1965104086 @default.
- W4283697788 cites W1969499061 @default.
- W4283697788 cites W1973332589 @default.
- W4283697788 cites W1985044730 @default.
- W4283697788 cites W1987893498 @default.
- W4283697788 cites W1992804143 @default.
- W4283697788 cites W1994784460 @default.
- W4283697788 cites W1995983036 @default.
- W4283697788 cites W1999410927 @default.
- W4283697788 cites W2005699902 @default.
- W4283697788 cites W2010480948 @default.
- W4283697788 cites W2012034410 @default.
- W4283697788 cites W2012582486 @default.
- W4283697788 cites W2013689372 @default.
- W4283697788 cites W2013782445 @default.
- W4283697788 cites W2014915378 @default.
- W4283697788 cites W2021437181 @default.
- W4283697788 cites W2033537630 @default.
- W4283697788 cites W2037966441 @default.
- W4283697788 cites W2055091294 @default.
- W4283697788 cites W2061637184 @default.
- W4283697788 cites W2065895195 @default.
- W4283697788 cites W2075905225 @default.
- W4283697788 cites W2076585749 @default.
- W4283697788 cites W2077723522 @default.
- W4283697788 cites W2078318363 @default.
- W4283697788 cites W2085347056 @default.
- W4283697788 cites W2085653746 @default.
- W4283697788 cites W2086281463 @default.
- W4283697788 cites W2089927445 @default.
- W4283697788 cites W2092083107 @default.
- W4283697788 cites W2100531566 @default.
- W4283697788 cites W2109352233 @default.
- W4283697788 cites W2113852915 @default.
- W4283697788 cites W2119116020 @default.
- W4283697788 cites W2122263641 @default.
- W4283697788 cites W2122299571 @default.
- W4283697788 cites W2128466784 @default.
- W4283697788 cites W2131350108 @default.
- W4283697788 cites W2135325995 @default.
- W4283697788 cites W2140034220 @default.
- W4283697788 cites W2143017330 @default.
- W4283697788 cites W2159821505 @default.
- W4283697788 cites W2164416830 @default.
- W4283697788 cites W2167704983 @default.
- W4283697788 cites W2189009044 @default.
- W4283697788 cites W2302586074 @default.
- W4283697788 cites W2409378722 @default.
- W4283697788 cites W2433244712 @default.
- W4283697788 cites W2467949190 @default.
- W4283697788 cites W2789228913 @default.
- W4283697788 cites W2797886191 @default.
- W4283697788 cites W2806713886 @default.
- W4283697788 cites W2900569176 @default.
- W4283697788 cites W2903675424 @default.
- W4283697788 cites W2913224919 @default.
- W4283697788 cites W2918155223 @default.
- W4283697788 cites W2939841075 @default.
- W4283697788 cites W2952666365 @default.
- W4283697788 cites W2968847312 @default.
- W4283697788 cites W2981020616 @default.
- W4283697788 cites W2999095881 @default.
- W4283697788 cites W3039695295 @default.
- W4283697788 cites W3113286963 @default.
- W4283697788 cites W3118634460 @default.
- W4283697788 cites W3135882325 @default.
- W4283697788 cites W3136131935 @default.
- W4283697788 cites W4214878920 @default.
- W4283697788 cites W4253779214 @default.
- W4283697788 cites W55744857 @default.
- W4283697788 doi "https://doi.org/10.1007/s00432-022-04127-6" @default.
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