Matches in SemOpenAlex for { <https://semopenalex.org/work/W4283704705> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W4283704705 abstract "Abstract Purpose Recent studies have shown that G protein-regulated inducer of neurite outgrowth 1 (GPRIN1) regulates cancer proliferation and migration. However, there is limited research regarding its role in gallbladder cancer (GBC). We aimed to investigate the mechanism by which GPRIN1 promotes GBC cell proliferation and migration. Methods Immunohistochemistry was performed to visualize in situ GPRIN1 expression in 65 human GBC tissues and 59 corresponding normal tissues. The Kaplan–Meier method was used to analyze the correlation between GPRIN1 and overall survival. Cell proliferation (Celigo and MTT), apoptosis (FACS), and cell migration and invasion assays and a subcutaneous xenograft tumor model were used to define the effects of GPRIN1 downregulation in GBC cells in vitro and in vivo. Coimmunoprecipitation assays, mass spectrometry and Western blotting were performed to identify GPRIN1-interacting proteins. Cell proliferation, MTT and Transwell assays were performed to verify the functional recovery effect of cyclin-dependent protein kinase 1 (CDK1) on GPRIN1. Proteomic analysis of differentially expressed proteins and/or phosphorylated peptides was performed. Results GPRIN1 is upregulated in GBC tumor tissues and high GPRIN1 expression is positively correlated with a poor prognosis in GBC patients. High GPRIN1 expression reinforced the proliferative and invasive capacities of GBC in vitro and in vivo. Coimmunoprecipitation assays, mass spectrometry and Western blotting validated the direct interaction between GPRIN1 and CDK1. Cell proliferation, MTT and Transwell assays suggested that CDK1 exerts a functional recovery effect on GPRIN1. Proteomic analysis revealed the involvement of CDK1 in many biological processes and cellular pathways. Conclusions GPRIN1 promotes the invasion and metastasis of GBC cells via CDK1 pathway activation." @default.
- W4283704705 created "2022-06-30" @default.
- W4283704705 creator A5001676555 @default.
- W4283704705 creator A5019708503 @default.
- W4283704705 creator A5022704617 @default.
- W4283704705 creator A5027601293 @default.
- W4283704705 creator A5048732909 @default.
- W4283704705 creator A5052024256 @default.
- W4283704705 creator A5078909212 @default.
- W4283704705 creator A5085882601 @default.
- W4283704705 creator A5091003789 @default.
- W4283704705 creator A5091637996 @default.
- W4283704705 date "2022-06-29" @default.
- W4283704705 modified "2023-09-30" @default.
- W4283704705 title "GPRIN1 promotes gallbladder cancer progression through the CDK1 pathway" @default.
- W4283704705 doi "https://doi.org/10.21203/rs.3.rs-1782796/v1" @default.
- W4283704705 hasPublicationYear "2022" @default.
- W4283704705 type Work @default.
- W4283704705 citedByCount "0" @default.
- W4283704705 crossrefType "posted-content" @default.
- W4283704705 hasAuthorship W4283704705A5001676555 @default.
- W4283704705 hasAuthorship W4283704705A5019708503 @default.
- W4283704705 hasAuthorship W4283704705A5022704617 @default.
- W4283704705 hasAuthorship W4283704705A5027601293 @default.
- W4283704705 hasAuthorship W4283704705A5048732909 @default.
- W4283704705 hasAuthorship W4283704705A5052024256 @default.
- W4283704705 hasAuthorship W4283704705A5078909212 @default.
- W4283704705 hasAuthorship W4283704705A5085882601 @default.
- W4283704705 hasAuthorship W4283704705A5091003789 @default.
- W4283704705 hasAuthorship W4283704705A5091637996 @default.
- W4283704705 hasBestOaLocation W42837047051 @default.
- W4283704705 hasConcept C104317684 @default.
- W4283704705 hasConcept C120504264 @default.
- W4283704705 hasConcept C121608353 @default.
- W4283704705 hasConcept C127561419 @default.
- W4283704705 hasConcept C137738243 @default.
- W4283704705 hasConcept C1491633281 @default.
- W4283704705 hasConcept C153911025 @default.
- W4283704705 hasConcept C185592680 @default.
- W4283704705 hasConcept C2776534502 @default.
- W4283704705 hasConcept C2780783641 @default.
- W4283704705 hasConcept C29537977 @default.
- W4283704705 hasConcept C502942594 @default.
- W4283704705 hasConcept C53227056 @default.
- W4283704705 hasConcept C54355233 @default.
- W4283704705 hasConcept C55493867 @default.
- W4283704705 hasConcept C62112901 @default.
- W4283704705 hasConcept C71829478 @default.
- W4283704705 hasConcept C81885089 @default.
- W4283704705 hasConcept C85265743 @default.
- W4283704705 hasConcept C86803240 @default.
- W4283704705 hasConcept C95444343 @default.
- W4283704705 hasConceptScore W4283704705C104317684 @default.
- W4283704705 hasConceptScore W4283704705C120504264 @default.
- W4283704705 hasConceptScore W4283704705C121608353 @default.
- W4283704705 hasConceptScore W4283704705C127561419 @default.
- W4283704705 hasConceptScore W4283704705C137738243 @default.
- W4283704705 hasConceptScore W4283704705C1491633281 @default.
- W4283704705 hasConceptScore W4283704705C153911025 @default.
- W4283704705 hasConceptScore W4283704705C185592680 @default.
- W4283704705 hasConceptScore W4283704705C2776534502 @default.
- W4283704705 hasConceptScore W4283704705C2780783641 @default.
- W4283704705 hasConceptScore W4283704705C29537977 @default.
- W4283704705 hasConceptScore W4283704705C502942594 @default.
- W4283704705 hasConceptScore W4283704705C53227056 @default.
- W4283704705 hasConceptScore W4283704705C54355233 @default.
- W4283704705 hasConceptScore W4283704705C55493867 @default.
- W4283704705 hasConceptScore W4283704705C62112901 @default.
- W4283704705 hasConceptScore W4283704705C71829478 @default.
- W4283704705 hasConceptScore W4283704705C81885089 @default.
- W4283704705 hasConceptScore W4283704705C85265743 @default.
- W4283704705 hasConceptScore W4283704705C86803240 @default.
- W4283704705 hasConceptScore W4283704705C95444343 @default.
- W4283704705 hasLocation W42837047051 @default.
- W4283704705 hasOpenAccess W4283704705 @default.
- W4283704705 hasPrimaryLocation W42837047051 @default.
- W4283704705 hasRelatedWork W1969869963 @default.
- W4283704705 hasRelatedWork W2162489064 @default.
- W4283704705 hasRelatedWork W2348042013 @default.
- W4283704705 hasRelatedWork W2393801921 @default.
- W4283704705 hasRelatedWork W3029549195 @default.
- W4283704705 hasRelatedWork W3145696671 @default.
- W4283704705 hasRelatedWork W3171556590 @default.
- W4283704705 hasRelatedWork W4280493527 @default.
- W4283704705 hasRelatedWork W4283704705 @default.
- W4283704705 hasRelatedWork W2352200605 @default.
- W4283704705 isParatext "false" @default.
- W4283704705 isRetracted "false" @default.
- W4283704705 workType "article" @default.