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- W4283704859 abstract "Abstract WNT signaling is important for regulation of embryonic development. The most abundant WNT gene expressed in the bovine endometrium during the preimplantation period is WNT5A. One objective was to determine whether WNT5A regulates competence of the bovine preimplantation embryo to become a blastocyst and alters the number of cells in the inner cell mass and trophectoderm. A second objective was to delineate features of the cell-signaling mechanisms involved in WNT5A actions. WNT5A caused a concentration-dependent increase in the proportion of embryos developing to the blastocyst stage and in the number of inner cell mass cells in the resultant blastocysts. A concentration of 200 ng/mL was most effective, and a higher concentration of 400 ng/mL was not stimulatory. Bovine serum albumin in culture reduced the magnitude of effects of WNT5A on development to the blastocyst stage. WNT5A affected expression of 173 genes at the morula stage; all were upregulated by WNT5A. Many of the upregulated genes were associated with cell signaling. Actions of WNT5A on development to the blastocyst stage were suppressed by a Rho-associated coiled-coil kinase (ROCK) signaling inhibitor, suggesting that WNT5A acts through Ras homology gene family member A (RhoA)/ROCK signaling. Other experiments indicated that actions of WNT5A are independent of the canonical β-catenin signaling pathway and RAC1/c-Jun N-terminal kinase (JNK) signaling. This is the first report outlining the actions of WNT5A to alter the development of the mammalian embryo. These findings provide insights into how embryokines regulate maternal–embryonic communication." @default.
- W4283704859 created "2022-06-30" @default.
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- W4283704859 date "2022-06-28" @default.
- W4283704859 modified "2023-10-18" @default.
- W4283704859 title "Actions of WNT family member 5A to regulate characteristics of development of the bovine preimplantation embryo" @default.
- W4283704859 cites W1625738754 @default.
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- W4283704859 cites W1886443720 @default.
- W4283704859 cites W1907833971 @default.
- W4283704859 cites W1965088114 @default.
- W4283704859 cites W1967053032 @default.
- W4283704859 cites W1971767775 @default.
- W4283704859 cites W1982339802 @default.
- W4283704859 cites W1989918771 @default.
- W4283704859 cites W2000368578 @default.
- W4283704859 cites W2005440429 @default.
- W4283704859 cites W2005468985 @default.
- W4283704859 cites W2006037174 @default.
- W4283704859 cites W2007218422 @default.
- W4283704859 cites W2008991071 @default.
- W4283704859 cites W2009684443 @default.
- W4283704859 cites W2010422006 @default.
- W4283704859 cites W2013891209 @default.
- W4283704859 cites W2024499742 @default.
- W4283704859 cites W2027829948 @default.
- W4283704859 cites W2028256918 @default.
- W4283704859 cites W2035569732 @default.
- W4283704859 cites W2039435520 @default.
- W4283704859 cites W2040480702 @default.
- W4283704859 cites W2071331098 @default.
- W4283704859 cites W2071397057 @default.
- W4283704859 cites W2074061817 @default.
- W4283704859 cites W2079945043 @default.
- W4283704859 cites W2083935868 @default.
- W4283704859 cites W2086316954 @default.
- W4283704859 cites W2089426706 @default.
- W4283704859 cites W2092630678 @default.
- W4283704859 cites W2099681999 @default.
- W4283704859 cites W2102278945 @default.
- W4283704859 cites W2106778371 @default.
- W4283704859 cites W2112058982 @default.
- W4283704859 cites W2113263838 @default.
- W4283704859 cites W2119284420 @default.
- W4283704859 cites W2124485554 @default.
- W4283704859 cites W2128181658 @default.
- W4283704859 cites W2130410032 @default.
- W4283704859 cites W2130437709 @default.
- W4283704859 cites W2131086923 @default.
- W4283704859 cites W2132576204 @default.
- W4283704859 cites W2138207763 @default.
- W4283704859 cites W2139211316 @default.
- W4283704859 cites W2140667637 @default.
- W4283704859 cites W2142685635 @default.
- W4283704859 cites W2150780033 @default.
- W4283704859 cites W2157364449 @default.
- W4283704859 cites W2161225294 @default.
- W4283704859 cites W2214074259 @default.
- W4283704859 cites W2221346445 @default.
- W4283704859 cites W2270233522 @default.
- W4283704859 cites W2512172172 @default.
- W4283704859 cites W2560749859 @default.
- W4283704859 cites W2568484384 @default.
- W4283704859 cites W2604201438 @default.
- W4283704859 cites W2616856840 @default.
- W4283704859 cites W2618542783 @default.
- W4283704859 cites W266824605 @default.
- W4283704859 cites W2732528012 @default.
- W4283704859 cites W2750887532 @default.
- W4283704859 cites W2767510416 @default.
- W4283704859 cites W2789772898 @default.
- W4283704859 cites W2849676904 @default.
- W4283704859 cites W2865334598 @default.
- W4283704859 cites W2884595216 @default.
- W4283704859 cites W2884618837 @default.
- W4283704859 cites W2897840948 @default.
- W4283704859 cites W2952901868 @default.
- W4283704859 cites W2967150294 @default.
- W4283704859 cites W2980616295 @default.
- W4283704859 cites W2981083645 @default.
- W4283704859 cites W3032795391 @default.
- W4283704859 cites W3092864602 @default.
- W4283704859 cites W3112725452 @default.
- W4283704859 cites W3158539122 @default.
- W4283704859 cites W3164820646 @default.
- W4283704859 cites W3202756866 @default.
- W4283704859 cites W3205066567 @default.
- W4283704859 doi "https://doi.org/10.1093/biolre/ioac127" @default.
- W4283704859 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35765196" @default.
- W4283704859 hasPublicationYear "2022" @default.
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