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- W4283709003 abstract "Collection, preservation, and shipment of histological specimens in low-resource settings is challenging. We present a novel method that achieved excellent preservation of placental specimens from rural Mali by using formalin fixation, ethanol dehydration, and long-term storage in a solar-powered freezer. Sample preservation success was 92%, permitting evaluation of current and past malaria infection, anemia, placental maturity, and inflammation. Using RNAscope® hybridization we were able to visualize cell-specific gene expression patterns in the formalin-fixed paraffin-embedded (FFPE) specimens. Additionally, our method entailed mirrored sampling from the two cut faces of a cotyledon, one for the FFPE workflows and the other for storage in RNAlater™ and RNA-seq." @default.
- W4283709003 created "2022-06-30" @default.
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- W4283709003 date "2022-06-29" @default.
- W4283709003 modified "2023-09-28" @default.
- W4283709003 title "A new method for the sampling and preservation of placental specimens in low-resource settings for the identification of <i>P. falciparum</i> and analysis of nucleic acids" @default.
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- W4283709003 doi "https://doi.org/10.1080/01478885.2022.2088191" @default.
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