Matches in SemOpenAlex for { <https://semopenalex.org/work/W4283729462> ?p ?o ?g. }
- W4283729462 abstract "ABSTRACT TRIM proteins are the largest family of E3 ligases in mammals. They include the intracellular antibody receptor TRIM21, which is responsible for mediating targeted protein degradation during Trim-Away. Despite their importance, the ubiquitination mechanism of TRIM ligases has remained elusive. Here we show that while TRIM21 activation results in ubiquitination of both ligase and substrate, autoubiquitination is regulatory and not required for substrate degradation. Substrate binding stimulates N-terminal RING autoubiquitination by the E2 Ube2W, but when inhibited by N-terminal acetylation this prevents neither substrate ubiquitination nor degradation and has no impact on TRIM21 antiviral activity. Instead, uncoupling ligase and substrate degradation prevents ligase recycling and extends functional persistence in cells. Substrate ubiquitination mediates degradation but Trim-Away efficiently degrades lysine-less substrates, suggesting a non-canonical ubiquitination mechanism explains its broad substrate specificity." @default.
- W4283729462 created "2022-07-01" @default.
- W4283729462 creator A5009997967 @default.
- W4283729462 creator A5017159718 @default.
- W4283729462 creator A5017777250 @default.
- W4283729462 creator A5029489083 @default.
- W4283729462 creator A5036377487 @default.
- W4283729462 creator A5037503418 @default.
- W4283729462 creator A5047139329 @default.
- W4283729462 creator A5053393375 @default.
- W4283729462 creator A5054269276 @default.
- W4283729462 creator A5060010220 @default.
- W4283729462 creator A5060600351 @default.
- W4283729462 creator A5085340246 @default.
- W4283729462 date "2022-06-29" @default.
- W4283729462 modified "2023-10-06" @default.
- W4283729462 title "Trim-Away degrades lysine-less substrates without requiring ligase autoubiquitination" @default.
- W4283729462 cites W1527682346 @default.
- W4283729462 cites W1547431965 @default.
- W4283729462 cites W1912867168 @default.
- W4283729462 cites W1967761542 @default.
- W4283729462 cites W1999029823 @default.
- W4283729462 cites W2000694202 @default.
- W4283729462 cites W2022754521 @default.
- W4283729462 cites W2032499969 @default.
- W4283729462 cites W2033702347 @default.
- W4283729462 cites W2050723187 @default.
- W4283729462 cites W2051839070 @default.
- W4283729462 cites W2052172534 @default.
- W4283729462 cites W2057040546 @default.
- W4283729462 cites W2064956147 @default.
- W4283729462 cites W2072221102 @default.
- W4283729462 cites W2082314789 @default.
- W4283729462 cites W2084132171 @default.
- W4283729462 cites W2088854979 @default.
- W4283729462 cites W2101625991 @default.
- W4283729462 cites W2106246537 @default.
- W4283729462 cites W2109954733 @default.
- W4283729462 cites W2116773729 @default.
- W4283729462 cites W2122691473 @default.
- W4283729462 cites W2136623638 @default.
- W4283729462 cites W2144081223 @default.
- W4283729462 cites W2148372587 @default.
- W4283729462 cites W2151766144 @default.
- W4283729462 cites W2153857286 @default.
- W4283729462 cites W2154197653 @default.
- W4283729462 cites W2155768478 @default.
- W4283729462 cites W2156829363 @default.
- W4283729462 cites W2169707043 @default.
- W4283729462 cites W2180229411 @default.
- W4283729462 cites W2249738812 @default.
- W4283729462 cites W2291224302 @default.
- W4283729462 cites W2294015155 @default.
- W4283729462 cites W2419339143 @default.
- W4283729462 cites W2530977520 @default.
- W4283729462 cites W2562210432 @default.
- W4283729462 cites W2769995426 @default.
- W4283729462 cites W2788812105 @default.
- W4283729462 cites W2797773019 @default.
- W4283729462 cites W2801545642 @default.
- W4283729462 cites W2802635892 @default.
- W4283729462 cites W2876540176 @default.
- W4283729462 cites W2902081417 @default.
- W4283729462 cites W2921660925 @default.
- W4283729462 cites W2938447226 @default.
- W4283729462 cites W2949880109 @default.
- W4283729462 cites W2976500897 @default.
- W4283729462 cites W2977821985 @default.
- W4283729462 cites W2980699505 @default.
- W4283729462 cites W2991127821 @default.
- W4283729462 cites W2997879126 @default.
- W4283729462 cites W3065954717 @default.
- W4283729462 cites W3115202979 @default.
- W4283729462 cites W3119543647 @default.
- W4283729462 cites W3130816211 @default.
- W4283729462 cites W3150184953 @default.
- W4283729462 cites W3160147040 @default.
- W4283729462 cites W3184017175 @default.
- W4283729462 cites W3216896237 @default.
- W4283729462 cites W4205264786 @default.
- W4283729462 cites W4210483783 @default.
- W4283729462 cites W4220937155 @default.
- W4283729462 cites W4229441458 @default.
- W4283729462 doi "https://doi.org/10.1101/2022.06.29.498105" @default.
- W4283729462 hasPublicationYear "2022" @default.
- W4283729462 type Work @default.
- W4283729462 citedByCount "1" @default.
- W4283729462 countsByYear W42837294622023 @default.
- W4283729462 crossrefType "posted-content" @default.
- W4283729462 hasAuthorship W4283729462A5009997967 @default.
- W4283729462 hasAuthorship W4283729462A5017159718 @default.
- W4283729462 hasAuthorship W4283729462A5017777250 @default.
- W4283729462 hasAuthorship W4283729462A5029489083 @default.
- W4283729462 hasAuthorship W4283729462A5036377487 @default.
- W4283729462 hasAuthorship W4283729462A5037503418 @default.
- W4283729462 hasAuthorship W4283729462A5047139329 @default.
- W4283729462 hasAuthorship W4283729462A5053393375 @default.
- W4283729462 hasAuthorship W4283729462A5054269276 @default.
- W4283729462 hasAuthorship W4283729462A5060010220 @default.
- W4283729462 hasAuthorship W4283729462A5060600351 @default.