Matches in SemOpenAlex for { <https://semopenalex.org/work/W4283743191> ?p ?o ?g. }
- W4283743191 abstract "Abstract The nascent polypeptide-associated complex (NAC) consisting of α- and β-subunits is an essential ribosome-associated protein conserved in eukaryotes. NAC is a ubiquitously expressed co-translational regulator of nascent protein folding and sorting providing for homeostasis of cellular proteins. Here we report on discovering the germline-specific NACαβ paralogs (gNACs), whose β-subunits, non-distinguishable by ordinary immunodetection, are encoded by five highly homologous gene copies, while the α-subunit is encoded by a single αNAC gene. The gNAC expression is detected in the primordial embryonic and adult gonads via immunostaining. The germline-specific α and β subunits differ from the ubiquitously expressed paralogs by the extended intrinsically disordered regions (IDRs) acquired at the N- and C-termini of the coding regions, predicted to be phosphorylated. The presence of distinct phosphorylated isoforms of gNAC-β subunits is confirmed by comparing of their profiles by 2D-isoeletrofocusing resolution before and after phosphatase treatment of testis ribosomes. We revealed that the predicted S/T sites of phosphorylation in the individual orthologous IDRs of gNAC-β sequences of Drosophila species are positionally conserved despite these disordered regions are drastically different. We propose the IDR-dependent molecular crowding and specific coordination of NAC and other proteostasis regulatory factors at the ribosomes of germinal cells. Our findings imply that there may be a functional crosstalk between the germinal and ubiquitous α- and β-subunits based on assessing their depletion effects on the fly viability and gonad development." @default.
- W4283743191 created "2022-07-02" @default.
- W4283743191 creator A5020453750 @default.
- W4283743191 creator A5023828983 @default.
- W4283743191 creator A5035137022 @default.
- W4283743191 creator A5054307674 @default.
- W4283743191 creator A5055746127 @default.
- W4283743191 creator A5056961011 @default.
- W4283743191 creator A5071833760 @default.
- W4283743191 creator A5078882430 @default.
- W4283743191 creator A5089935846 @default.
- W4283743191 creator A5056639970 @default.
- W4283743191 date "2022-07-01" @default.
- W4283743191 modified "2023-09-26" @default.
- W4283743191 title "Extended disordered regions of ribosome-associated NAC proteins paralogs belong only to the germline in Drosophila melanogaster" @default.
- W4283743191 cites W1818413434 @default.
- W4283743191 cites W1834361763 @default.
- W4283743191 cites W1980780093 @default.
- W4283743191 cites W1991113512 @default.
- W4283743191 cites W1994528729 @default.
- W4283743191 cites W2002974480 @default.
- W4283743191 cites W2031611770 @default.
- W4283743191 cites W2039513425 @default.
- W4283743191 cites W2042745266 @default.
- W4283743191 cites W2051190420 @default.
- W4283743191 cites W2052112605 @default.
- W4283743191 cites W2053820259 @default.
- W4283743191 cites W2071038701 @default.
- W4283743191 cites W2084433606 @default.
- W4283743191 cites W2089897307 @default.
- W4283743191 cites W2101291993 @default.
- W4283743191 cites W2103169255 @default.
- W4283743191 cites W2104582907 @default.
- W4283743191 cites W2107040871 @default.
- W4283743191 cites W2115888213 @default.
- W4283743191 cites W2122132730 @default.
- W4283743191 cites W2141052558 @default.
- W4283743191 cites W2145773480 @default.
- W4283743191 cites W2158361034 @default.
- W4283743191 cites W2158592738 @default.
- W4283743191 cites W2158714788 @default.
- W4283743191 cites W2159368494 @default.
- W4283743191 cites W2160378127 @default.
- W4283743191 cites W2162038188 @default.
- W4283743191 cites W2174043882 @default.
- W4283743191 cites W2186209672 @default.
- W4283743191 cites W2276471287 @default.
- W4283743191 cites W2300545781 @default.
- W4283743191 cites W2537623931 @default.
- W4283743191 cites W2748003993 @default.
- W4283743191 cites W2788194767 @default.
- W4283743191 cites W2793630394 @default.
- W4283743191 cites W2797356047 @default.
- W4283743191 cites W2798148204 @default.
- W4283743191 cites W2800613882 @default.
- W4283743191 cites W2803300419 @default.
- W4283743191 cites W2806884808 @default.
- W4283743191 cites W2887449097 @default.
- W4283743191 cites W2888885392 @default.
- W4283743191 cites W2902272528 @default.
- W4283743191 cites W2914590607 @default.
- W4283743191 cites W2920252328 @default.
- W4283743191 cites W2936977611 @default.
- W4283743191 cites W2941072767 @default.
- W4283743191 cites W2950365857 @default.
- W4283743191 cites W2954926994 @default.
- W4283743191 cites W2955418283 @default.
- W4283743191 cites W2966849264 @default.
- W4283743191 cites W2971695115 @default.
- W4283743191 cites W2975112817 @default.
- W4283743191 cites W2988357750 @default.
- W4283743191 cites W2989978582 @default.
- W4283743191 cites W3024551129 @default.
- W4283743191 cites W3045402435 @default.
- W4283743191 cites W3048941542 @default.
- W4283743191 cites W3090522312 @default.
- W4283743191 cites W3090646187 @default.
- W4283743191 cites W3092787915 @default.
- W4283743191 cites W3098142057 @default.
- W4283743191 cites W3105750359 @default.
- W4283743191 cites W3177828909 @default.
- W4283743191 cites W3183475563 @default.
- W4283743191 cites W3210080058 @default.
- W4283743191 cites W4200253823 @default.
- W4283743191 cites W4213437949 @default.
- W4283743191 cites W4226126233 @default.
- W4283743191 cites W4241316979 @default.
- W4283743191 doi "https://doi.org/10.1038/s41598-022-15233-3" @default.
- W4283743191 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35778515" @default.
- W4283743191 hasPublicationYear "2022" @default.
- W4283743191 type Work @default.
- W4283743191 citedByCount "1" @default.
- W4283743191 countsByYear W42837431912022 @default.
- W4283743191 crossrefType "journal-article" @default.
- W4283743191 hasAuthorship W4283743191A5020453750 @default.
- W4283743191 hasAuthorship W4283743191A5023828983 @default.
- W4283743191 hasAuthorship W4283743191A5035137022 @default.
- W4283743191 hasAuthorship W4283743191A5054307674 @default.
- W4283743191 hasAuthorship W4283743191A5055746127 @default.
- W4283743191 hasAuthorship W4283743191A5056639970 @default.