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• W4283747478 abstract "Abstract A commercial plant probiotic product was developed employing Bacillus subtilis CW-S in submerged fermentation. The effects of molasses and urea on cell growth were investigated with the goal of low-cost manufacturing. Plackett–Burman and Central-Composite Design (CCD) were utilized to optimize production parameters to maximize productivity. The stability of the formulated product and its efficacy in cultivating minituber in aeroponics and industrial-grade potatoes in the field were assessed. The results showed that the medium BS10 (molasses and urea) produced satisfactory cell density (7.19 × 10 8 CFU/mL) as compared to the control (1.51 × 10 7 CFU/mL) and BS1-BS9 (expensive) media (1.84 × 10 7 –1.37 × 10 9 CFU/mL). According to validated CCD results, optimized parameters fitted well in pilot (300 L; 2.05 × 10 9 CFU/mL) and industrial (3000 L; 2.01 × 10 9 CFU/mL) bioreactors, resulting in a two-fold increase in cell concentration over laboratory (9.84 × 10 8 CFU/mL) bioreactors. In aeroponics, CW-S produced excellent results, with a significant increase in the quantity and weight of minitubers and the survival rate of transplanted plantlets. In a field test, the yield of industrial-grade (> 55 mm) potatoes was increased with a reduction in fertilizer dose. Overall, the findings suggest that CW-S can be produced commercially utilizing the newly developed media and optimized conditions, making plant probiotics more cost-effective and accessible to farmers for crop cultivation, particularly in aeroponic minituber and industrial-grade potato production." @default.
• W4283747478 created "2022-07-02" @default.
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• W4283747478 date "2022-07-01" @default.
• W4283747478 modified "2023-10-09" @default.
• W4283747478 title "Optimization of industrial (3000 L) production of Bacillus subtilis CW-S and its novel application for minituber and industrial-grade potato cultivation" @default.
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• W4283747478 doi "https://doi.org/10.1038/s41598-022-15366-5" @default.
• W4283747478 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35778426" @default.
• W4283747478 hasPublicationYear "2022" @default.
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