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- W4283753007 abstract "Abstract Issues related to snakebite has for long been of high economic and medical importance. Management and treatment of snake envenomation has always been an issue, especially in remote areas, where anti-snake venom and facilities for its storage are not available, coupled with the high specificity and instability of anti-venom. Venom enzymes are usually responsible for almost all the activity of snake venom after bite. This research was aimed at evaluation and characterization of Hyaluronidase and Phospholipase A 2 (PLA2) Enzymes of Echis ocellatus and Naja nigricollis snake venoms. Isolation and purification of these enzymes were done using a two-step process which included gel filtration on Sephadex G-75, active fractions were applied to ion–exchange chromatography on DEAE (Diethylaminoethyl) cellulose. Individual active fractions of each venom, were then subjected to SDS–PAGE for molecular weight extrapolation. Enzyme characterization was done on the two isolated enzymes for the two snake venom used. N. nigricollis enzymes were revealed to have an optimum temperature of 35 o C and 55 o C, while that of E. ocellatus had 37 o C and 40 o C, with a pH of 3.5 and 8.0 for both the venom enzymes. Velocity kinetics carried out shows that N. nigricollis PLA 2 has the highest V max value of 30.567mg/min, while E. ocellatus PLA 2 however has the highest K m value of 4.5378mg/ml. Purification and characterization done in this research has revealed/confirmed that these venoms contains Hyaluronidase and PLA 2 enzymes, giving a better perception on this enzymes which will aids in the management and treatment of snake envenomation from these snakes." @default.
- W4283753007 created "2022-07-02" @default.
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- W4283753007 date "2022-07-01" @default.
- W4283753007 modified "2023-09-26" @default.
- W4283753007 title "Isolation, Purification and Characterization of Hyaluronidase and Phospholipase a 2 Enzymes of Echis Ocellatus and Naja Nigricollis Snake Venoms" @default.
- W4283753007 doi "https://doi.org/10.21203/rs.3.rs-1725908/v1" @default.
- W4283753007 hasPublicationYear "2022" @default.
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