Matches in SemOpenAlex for { <https://semopenalex.org/work/W4283759871> ?p ?o ?g. }
- W4283759871 abstract "Abstract The shotgun proteomic analysis is currently the most promising single-cell protein sequencing technology, however its identification level of ∼1000 proteins per cell is still insufficient for practical applications. Here, we develop a pick-up single-cell proteomic analysis (PiSPA) workflow to achieve a deep identification capable of quantifying up to 3000 protein groups in a tumor cell using the label-free quantitative method. The PiSPA workflow is specially established for single-cell samples mainly based on a nanoliter-scale microfluidic liquid handling robot, capable of achieving single-cell capture, pretreatment and injection under the pick-up operation strategy. Using this customized workflow with remarkable improvement in protein identification, 1804-3349, 1778-3049 and 1074-2487 protein groups are quantified in single A549 cells ( n = 37), HeLa cells ( n = 44) and U2OS cells ( n = 27), respectively. Benefiting from the flexible cell picking-up ability, we study tumor cell migration at the single cell proteome level, demonstrating the potential in practical biological research from single-cell insight." @default.
- W4283759871 created "2022-07-02" @default.
- W4283759871 creator A5000091003 @default.
- W4283759871 creator A5019221209 @default.
- W4283759871 creator A5020993784 @default.
- W4283759871 creator A5032525569 @default.
- W4283759871 creator A5042285468 @default.
- W4283759871 creator A5043028677 @default.
- W4283759871 creator A5048248480 @default.
- W4283759871 creator A5051941824 @default.
- W4283759871 creator A5060311326 @default.
- W4283759871 creator A5071357730 @default.
- W4283759871 creator A5079594267 @default.
- W4283759871 creator A5086358309 @default.
- W4283759871 creator A5087757082 @default.
- W4283759871 creator A5090586704 @default.
- W4283759871 date "2022-06-30" @default.
- W4283759871 modified "2023-09-25" @default.
- W4283759871 title "Pick-up Single-Cell Proteomic Analysis for Quantifying up to 3000 Proteins in a Tumor Cell" @default.
- W4283759871 cites W2015329331 @default.
- W4283759871 cites W2019552331 @default.
- W4283759871 cites W2019689594 @default.
- W4283759871 cites W2022960597 @default.
- W4283759871 cites W2024626836 @default.
- W4283759871 cites W2102212449 @default.
- W4283759871 cites W2103017472 @default.
- W4283759871 cites W2121690903 @default.
- W4283759871 cites W2135937351 @default.
- W4283759871 cites W2139838862 @default.
- W4283759871 cites W2164963523 @default.
- W4283759871 cites W2228137772 @default.
- W4283759871 cites W2502148278 @default.
- W4283759871 cites W2725388488 @default.
- W4283759871 cites W2758683424 @default.
- W4283759871 cites W2790054076 @default.
- W4283759871 cites W2804399702 @default.
- W4283759871 cites W2893216910 @default.
- W4283759871 cites W2899391438 @default.
- W4283759871 cites W2900569176 @default.
- W4283759871 cites W2901313844 @default.
- W4283759871 cites W2920030348 @default.
- W4283759871 cites W2942397792 @default.
- W4283759871 cites W2946847644 @default.
- W4283759871 cites W2951821937 @default.
- W4283759871 cites W2952907530 @default.
- W4283759871 cites W2953064750 @default.
- W4283759871 cites W2972334216 @default.
- W4283759871 cites W2998738108 @default.
- W4283759871 cites W2999537573 @default.
- W4283759871 cites W3009818459 @default.
- W4283759871 cites W3021060601 @default.
- W4283759871 cites W3041326933 @default.
- W4283759871 cites W3048389895 @default.
- W4283759871 cites W3110251663 @default.
- W4283759871 cites W3112047697 @default.
- W4283759871 cites W3117135844 @default.
- W4283759871 cites W3121536135 @default.
- W4283759871 cites W3123837475 @default.
- W4283759871 cites W3159562858 @default.
- W4283759871 cites W3177450887 @default.
- W4283759871 cites W3195015580 @default.
- W4283759871 cites W3206144875 @default.
- W4283759871 cites W3206193315 @default.
- W4283759871 cites W4205798555 @default.
- W4283759871 cites W4220999759 @default.
- W4283759871 cites W4294216483 @default.
- W4283759871 doi "https://doi.org/10.1101/2022.06.28.498038" @default.
- W4283759871 hasPublicationYear "2022" @default.
- W4283759871 type Work @default.
- W4283759871 citedByCount "3" @default.
- W4283759871 countsByYear W42837598712022 @default.
- W4283759871 countsByYear W42837598712023 @default.
- W4283759871 crossrefType "posted-content" @default.
- W4283759871 hasAuthorship W4283759871A5000091003 @default.
- W4283759871 hasAuthorship W4283759871A5019221209 @default.
- W4283759871 hasAuthorship W4283759871A5020993784 @default.
- W4283759871 hasAuthorship W4283759871A5032525569 @default.
- W4283759871 hasAuthorship W4283759871A5042285468 @default.
- W4283759871 hasAuthorship W4283759871A5043028677 @default.
- W4283759871 hasAuthorship W4283759871A5048248480 @default.
- W4283759871 hasAuthorship W4283759871A5051941824 @default.
- W4283759871 hasAuthorship W4283759871A5060311326 @default.
- W4283759871 hasAuthorship W4283759871A5071357730 @default.
- W4283759871 hasAuthorship W4283759871A5079594267 @default.
- W4283759871 hasAuthorship W4283759871A5086358309 @default.
- W4283759871 hasAuthorship W4283759871A5087757082 @default.
- W4283759871 hasAuthorship W4283759871A5090586704 @default.
- W4283759871 hasBestOaLocation W42837598711 @default.
- W4283759871 hasConcept C104317684 @default.
- W4283759871 hasConcept C104397665 @default.
- W4283759871 hasConcept C116834253 @default.
- W4283759871 hasConcept C1491633281 @default.
- W4283759871 hasConcept C177212765 @default.
- W4283759871 hasConcept C185592680 @default.
- W4283759871 hasConcept C2776950831 @default.
- W4283759871 hasConcept C2777366897 @default.
- W4283759871 hasConcept C41008148 @default.
- W4283759871 hasConcept C46111723 @default.
- W4283759871 hasConcept C55493867 @default.
- W4283759871 hasConcept C59822182 @default.