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- W4283805592 startingPage "111309" @default.
- W4283805592 abstract "Armeniaca sibirica is an important nut species with ecological and economic value. However, low fruit yield has become a significant obstacle to industrial production of A. sibirica. High and stable yield breeding via cross-pollination has become a solution for improving production. Therefore, pollen with high vitality is essential to ensure successful hybridization in the A. sibirica breeding program. In this study, we used pollen from a A. sibirica clone to optimize the composition of germination medium. The orthogonal assay test strategy (OATS) indicated an agar-solidified medium containing 15 g/100 mL sucrose, 0.01 g/100 mL boric acid, and 0.01 g/100 mL calcium nitrate at pH 6.0 was optimal for pollen germination. The pollen germination rate and tube length in the optimal medium were checked at seven different temperatures and times. The results indicated that 25 °C was the optimal temperature. Controlled unfreezing and rehydration experiments showed that the germination rate of cryopreserved pollen reached its highest value when pollen was thawed for 24 h at 4 °C and rehydrated for 4 h at 25 °C, demonstrating that unfreezing and rehydration greatly improved the vitality of pollen. A. sibirica pollen could remain high viability at 25 °C (4 weeks) or 4 °C (8 weeks) for short-term storage, and -20 °C and -80 °C were suitable temperatures for long-term storage (52 weeks). Our results can provide helpful information for pollen sources in the A. sibirica cross breeding program." @default.
- W4283805592 created "2022-07-06" @default.
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- W4283805592 date "2022-10-01" @default.
- W4283805592 modified "2023-09-30" @default.
- W4283805592 title "Optimization of In Vitro Germination and storage of Armeniaca sibirica Pollen" @default.
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- W4283805592 doi "https://doi.org/10.1016/j.scienta.2022.111309" @default.
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