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• W4283806082 abstract "Abstract Resistance of plant-pathogenic bacteria to classic antibiotics has prompted the search for suitable alternative antimicrobial substances. One promising strategy could be the use of purposely synthesized random peptide mixtures (RPMs). Six plant-pathogenic bacteria were cultivated and treated with two RPMs previously found to show antimicrobial activity mainly by bacterial membrane disruption. Here, we show that bacteria treated with RPMs showed partly remarkable changes in the fatty acid pattern while those unaffected did not. Quantitative changes could be verified by compound specific isotope analysis of δ 13 C values (‰). This technique was employed due to the characteristic feature of stronger bonds between heavier isotopes in (bio)chemical reactions. As a proof of concept, the increase in abundance of a fatty acid group after RPM treatment was accompanied with a decrease in the 13 C content and vice versa. We propose that our findings will help designing and synthesizing more selective antimicrobial peptides." @default.
• W4283806082 created "2022-07-06" @default.
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• W4283806082 date "2022-07-04" @default.
• W4283806082 modified "2023-09-30" @default.
• W4283806082 title "Stable isotope analysis confirms substantial changes in the fatty acid composition of bacteria treated with antimicrobial random peptide mixtures (RPMs)" @default.
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• W4283806082 doi "https://doi.org/10.1038/s41598-022-13134-z" @default.
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