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• W4284668649 abstract "Abstract We report an EN2-specific ( K d = 8.26 nM) aptamer, and a sensitive and specific enzyme-linked oligonucleotide assay (ELONA) for rapid and sensitive colorimetric detection of bladder and prostate cancer biomarker EN2 in urine. The assay relies on an aptamer-mediated hybridization chain reaction (HCR) to generate DNA nanostructures that bind to EN2 and simultaneously amplify signals. The assay can be performed within 2.5 h, and has a limit of detection of 0.34 nM in buffer and 2.69 nM in artificial urine. Moreover, this assay showed high specificity as it did not detect other urinary proteins, including biomarkers of other cancers. The proposed ELONA is inexpensive, highly reproducible, and has great chemical stability, so it may enable development of a simple, sensitive and accurate diagnostic tool to detect bladder and prostate cancers early." @default.
• W4284668649 created "2022-07-08" @default.
• W4284668649 creator A5007944436 @default.
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• W4284668649 date "2022-07-07" @default.
• W4284668649 modified "2023-10-14" @default.
• W4284668649 title "Aptamer-antibody hybrid ELONA that uses hybridization chain reaction to detect a urinary biomarker EN2 for bladder and prostate cancer" @default.
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• W4284668649 doi "https://doi.org/10.1038/s41598-022-15556-1" @default.
• W4284668649 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35798816" @default.
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