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• W4284885535 abstract "Abstract Background During the intervertebral disc (IVD) degeneration process, initial degenerative events occur at the extracellular matrix level, with the appearance of neoepitope peptides formed by the cleavage of aggrecan and collagen. This study aims to elucidate the spatial and temporal alterations of aggrecan and collagen neoepitope level during IVD degeneration. Methods Bovine caudal IVDs were cultured under four different conditions to mimic different degenerative situations. Samples cultured after 1‐ or 8‐days were collected for analysis. Human IVD samples were obtained from patients diagnosed with lumbar disc herniation (LDH) or adolescent idiopathic scoliosis (AIS). After immunohistochemical (IHC) staining of Aggrecanase Cleaved C‐terminus Aggrecan Neoepitope (NB100), MMP Cleaved C‐terminus Aggrecan Neoepitope (MMPCC), Collagen Type 1α1 1/4 fragment (C1α1) and Collagenase Cleaved Type I and II Collagen Neoepitope (C1,2C), staining optical density (OD)/area in extracellular matrix (OECM) and pericellular zone (OPCZ) were analyzed. Conditioned media of the bovine IVD was collected to measure protein level of inflammatory cytokines and C1,2C. Results For the bovine IVD sections, the aggrecan MMPCC neoepitope was accumulated in nucleus pulposus (NP) and cartilage endplate (EP) regions following mechanical overload in the one strike model after long‐term culture; as for the TNF‐α induced degeneration, the OECM and OPCZ of collagen C1,2C neoepitope was significantly increased in the outer AF region after long‐term culture; moreover, the C1,2C was only detected in conditioned medium from TNF‐α injection + Degenerative loading group after 8 days of culture. LDH patients showed higher MMPCC OECM in NP and higher C1,2C OECM in AF region compared with AIS patients. Conclusions In summary, aggrecan and collagen neoepitope profiles showed degeneration induction trigger‐ and region‐specific differences in the IVD organ culture models. Different IVD degeneration types are correlated with specific neoepitope expression profiles. These neoepitopes may be helpful as biomarkers of ECM degradation in early IVD degeneration and indicators of different degeneration phenotypes." @default.
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• W4284885535 date "2022-07-06" @default.
• W4284885535 modified "2023-09-26" @default.
• W4284885535 title "Neoepitope fragments as biomarkers for different phenotypes of intervertebral disc degeneration" @default.
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• W4284885535 cites W1971720270 @default.
• W4284885535 cites W1972496457 @default.
• W4284885535 cites W1972500391 @default.
• W4284885535 cites W1976429655 @default.
• W4284885535 cites W1979264581 @default.
• W4284885535 cites W1989115092 @default.
• W4284885535 cites W1991091521 @default.
• W4284885535 cites W2006051929 @default.
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• W4284885535 cites W2022051828 @default.
• W4284885535 cites W2024585159 @default.
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• W4284885535 cites W2044441423 @default.
• W4284885535 cites W2044797133 @default.
• W4284885535 cites W2052187864 @default.
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• W4284885535 cites W2076136227 @default.
• W4284885535 cites W2076549714 @default.
• W4284885535 cites W2078774951 @default.
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• W4284885535 cites W2111389970 @default.
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• W4284885535 cites W2189695522 @default.
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• W4284885535 cites W2757294010 @default.
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• W4284885535 doi "https://doi.org/10.1002/jsp2.1215" @default.
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