Matches in SemOpenAlex for { <https://semopenalex.org/work/W4284888082> ?p ?o ?g. }
- W4284888082 endingPage "E253" @default.
- W4284888082 startingPage "E242" @default.
- W4284888082 abstract "The aim of the present study was to investigate the fiber type-specific abundance of autophagy-related proteins after an overnight fast and following ingestion of a mixed meal in human skeletal muscle. Twelve overweight, healthy young male volunteers underwent a 3-h mixed meal tolerance test following an overnight fast. Blood samples were collected in the overnight-fasted state and throughout the 180-min postmeal period. Skeletal muscle biopsies were collected in the fasted state, and at 30 and 90 min after meal ingestion. Protein content of key autophagy markers and upstream signaling responses were measured in whole muscle and pooled single fibers using immunoblotting. In the fasted state, type I fibers displayed lower LC3B-I but higher LC3B-II abundance and higher LC3B-II/LC3B-I ratio compared with type II fibers (P < 0.05). However, there were no fiber type differences in p62/SQSTM1, unc-51 like autophagy activating kinase (ULK1), ATG5, or ATG12 (P > 0.05). Compared with the fasted state, there was a reduction in LC3B-II abundance, indicative of lower autophagosome content, in whole muscle and in both type I and type II fibers following meal ingestion (P < 0.05). This reduction in autophagosome content occurred alongside similar increases in p-AktS473 and p-mTORS2448 in both type I and type II muscle fibers (P < 0.05). In human skeletal muscle, type I fibers have a greater autophagosome content than type II fibers in the overnight-fasted state despite comparable abundance of other key upstream autophagy proteins. Autophagy is rapidly inhibited in both fiber types following the ingestion of a mixed meal.NEW & NOTEWORTHY This study examined the fiber type-specific content of key autophagy proteins in human muscle. We showed that markers of autophagosome content are higher in type I fibers in the overnight-fasted state, whereas autophagy is rapidly inhibited in both type I and type II fibers after the ingestion of a mixed meal." @default.
- W4284888082 created "2022-07-09" @default.
- W4284888082 creator A5008551790 @default.
- W4284888082 creator A5014868735 @default.
- W4284888082 creator A5034817349 @default.
- W4284888082 creator A5037576077 @default.
- W4284888082 creator A5037853404 @default.
- W4284888082 creator A5042899349 @default.
- W4284888082 creator A5043528134 @default.
- W4284888082 creator A5065307536 @default.
- W4284888082 creator A5084706531 @default.
- W4284888082 creator A5088878460 @default.
- W4284888082 date "2022-09-01" @default.
- W4284888082 modified "2023-10-15" @default.
- W4284888082 title "Muscle fiber type-specific autophagy responses following an overnight fast and mixed meal ingestion in human skeletal muscle" @default.
- W4284888082 cites W1553793487 @default.
- W4284888082 cites W1848438685 @default.
- W4284888082 cites W1970637701 @default.
- W4284888082 cites W1975200363 @default.
- W4284888082 cites W1985681603 @default.
- W4284888082 cites W1999211990 @default.
- W4284888082 cites W2014425292 @default.
- W4284888082 cites W2017969831 @default.
- W4284888082 cites W2025192214 @default.
- W4284888082 cites W2026414725 @default.
- W4284888082 cites W2036787961 @default.
- W4284888082 cites W2054594032 @default.
- W4284888082 cites W2059055543 @default.
- W4284888082 cites W2066697766 @default.
- W4284888082 cites W2082476985 @default.
- W4284888082 cites W2086879362 @default.
- W4284888082 cites W2087704606 @default.
- W4284888082 cites W2088184211 @default.
- W4284888082 cites W2103659405 @default.
- W4284888082 cites W2107056130 @default.
- W4284888082 cites W2115742671 @default.
- W4284888082 cites W2130025116 @default.
- W4284888082 cites W2137070227 @default.
- W4284888082 cites W2146887271 @default.
- W4284888082 cites W2147459821 @default.
- W4284888082 cites W2149771125 @default.
- W4284888082 cites W2152267376 @default.
- W4284888082 cites W2156144699 @default.
- W4284888082 cites W2158565718 @default.
- W4284888082 cites W2159569431 @default.
- W4284888082 cites W2159796957 @default.
- W4284888082 cites W2176311376 @default.
- W4284888082 cites W2179041531 @default.
- W4284888082 cites W2415429505 @default.
- W4284888082 cites W2470470829 @default.
- W4284888082 cites W2511883439 @default.
- W4284888082 cites W2593835158 @default.
- W4284888082 cites W2888964731 @default.
- W4284888082 cites W2937060859 @default.
- W4284888082 cites W2941224309 @default.
- W4284888082 cites W2953664298 @default.
- W4284888082 cites W2965478824 @default.
- W4284888082 cites W2998173680 @default.
- W4284888082 cites W3092966039 @default.
- W4284888082 cites W3108936441 @default.
- W4284888082 cites W3113557507 @default.
- W4284888082 cites W3210336483 @default.
- W4284888082 cites W4255692377 @default.
- W4284888082 cites W4282928367 @default.
- W4284888082 doi "https://doi.org/10.1152/ajpendo.00015.2022" @default.
- W4284888082 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35793481" @default.
- W4284888082 hasPublicationYear "2022" @default.
- W4284888082 type Work @default.
- W4284888082 citedByCount "2" @default.
- W4284888082 countsByYear W42848880822023 @default.
- W4284888082 crossrefType "journal-article" @default.
- W4284888082 hasAuthorship W4284888082A5008551790 @default.
- W4284888082 hasAuthorship W4284888082A5014868735 @default.
- W4284888082 hasAuthorship W4284888082A5034817349 @default.
- W4284888082 hasAuthorship W4284888082A5037576077 @default.
- W4284888082 hasAuthorship W4284888082A5037853404 @default.
- W4284888082 hasAuthorship W4284888082A5042899349 @default.
- W4284888082 hasAuthorship W4284888082A5043528134 @default.
- W4284888082 hasAuthorship W4284888082A5065307536 @default.
- W4284888082 hasAuthorship W4284888082A5084706531 @default.
- W4284888082 hasAuthorship W4284888082A5088878460 @default.
- W4284888082 hasConcept C126322002 @default.
- W4284888082 hasConcept C134018914 @default.
- W4284888082 hasConcept C168296220 @default.
- W4284888082 hasConcept C185592680 @default.
- W4284888082 hasConcept C190283241 @default.
- W4284888082 hasConcept C193230392 @default.
- W4284888082 hasConcept C203522944 @default.
- W4284888082 hasConcept C2778345441 @default.
- W4284888082 hasConcept C2779959927 @default.
- W4284888082 hasConcept C2781000418 @default.
- W4284888082 hasConcept C55493867 @default.
- W4284888082 hasConcept C71924100 @default.
- W4284888082 hasConcept C86803240 @default.
- W4284888082 hasConceptScore W4284888082C126322002 @default.
- W4284888082 hasConceptScore W4284888082C134018914 @default.
- W4284888082 hasConceptScore W4284888082C168296220 @default.
- W4284888082 hasConceptScore W4284888082C185592680 @default.