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• W4284890119 endingPage "100117" @default.
• W4284890119 startingPage "100117" @default.
• W4284890119 abstract "Transcriptome analysis is important for the quality improvement of edible fungi, however, the effect of polyethylene (PE) packaging on the preservation of Pleurotus eryngii at the transcriptome level still needs to be further investigated. In order to elucidate the effect of PE on delaying lignification of P. eryngii, this study focused on exploring effects of PE on enzymes and genes involved in lignification. The results showed that PE packaging delayed the deterioration of phenotype, color difference and weight loss rate of P. eryngii, inhibited lignin and H2O2 content and maintained firmness and cellulose content. The activities of PAL, POD, 4-CL were inhibited, and more laccase expression was activated. Fifty-five differentially expressed genes associated with laccase, multifunctional peroxidase (VP), POD and 4-CL were screened from 10 d, 20 d and 30 d transcriptome data. These results show that PE could inhibit lignification of P. eryngii by up-regulating laccase and VP related genes involved in lignin decomposition and down-regulating the expression of genes involved in lignin synthesis. Meanwhile, we employed Confocal Raman microspectroscopy (CRM) to realize lignin cell level visualization and PE could reduce lignin deposition and weaken the lignin signal bands formed. Therefore, PE can alleviate the lignification of P. eryngii during storage by regulating the expression of specific genes, advancing the understanding of lignification in postharvest P. eryngii at the molecular level, and CRM has the potential to detect the changes of P. eryngii cell wall." @default.
• W4284890119 created "2022-07-09" @default.
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• W4284890119 date "2022-12-01" @default.
• W4284890119 modified "2023-09-23" @default.
• W4284890119 title "Transcriptome analysis reveals the potential mechanism of polyethylene packing delaying lignification of Pleurotus eryngii" @default.
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• W4284890119 doi "https://doi.org/10.1016/j.fochms.2022.100117" @default.
• W4284890119 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35845151" @default.
• W4284890119 hasPublicationYear "2022" @default.
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