FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4284965210> ?p ?o ?g. }

• W4284965210 endingPage "e0270256" @default.
• W4284965210 startingPage "e0270256" @default.
• W4284965210 abstract "Introduction Thymine auxotrophic in vitro mutants of Escherichia coli were first reported in the mid-20th century. Later, thymine-dependent clinical strains of E . coli as well as other Enterobacterales, Enterococcus faecalis and Staphylococcus aureus have been recognized as the cause of persistent and recurrent infections. Objectives The aim of this study was to characterize the phenotype and investigate the molecular basis of thymine auxotrophy in ten E . coli isolates obtained at different time points from a patient with recurrent bloodstream infection (BSI) due to a chronic aortic graft infection treated with Trimethoprim/sulfamethoxazole (TMP-SMX). Methods Clinical data was obtained from hospital records. Growth characterization and antimicrobial susceptibility testing to TMP-SMX was performed on M9 agar and in MH broth with different thymine concentrations (0.5, 2, 5, 10 and 20 μg/mL), on Mueller-Hinton (MH) and blood agar. Whole genome sequencing (WGS) was performed on all E . coli isolates. Results E . coli were isolated from ten consecutive BSI episodes from a patient with chronic aortic graft infection. Six of these isolates were resistant to TMP-SMX when assayed on blood agar. Growth experiments with added thymine confirmed that these isolates were thymine-dependent ( thy- ), and revealed growth defects (slower growth rate and smaller colony size) in these isolates relative to thy+ isolates (n = 4). WGS indicated that all isolates were of the same clonal lineage of sequence type 7358. Genomic analysis revealed a G172C substitution in thyA in all TMP-SMX resistant isolates, while mutations affecting genes involved in the deoxyribose salvage pathway ( deoB and deoC ) were identified in eight isolates. Conclusion This case highlights the risk of resistance development to TMP-SMX, especially for long-term treatment, and the possible pitfalls in detection of growth-deficient subpopulations from chronic infections, which could lead to treatment failure." @default.
• W4284965210 created "2022-07-10" @default.
• W4284965210 creator A5003446319 @default.
• W4284965210 creator A5011018087 @default.
• W4284965210 creator A5030869812 @default.
• W4284965210 creator A5063386849 @default.
• W4284965210 creator A5079938824 @default.
• W4284965210 creator A5086464504 @default.
• W4284965210 creator A5087353619 @default.
• W4284965210 date "2022-07-08" @default.
• W4284965210 modified "2023-10-14" @default.
• W4284965210 title "Phenotypic and genotypic characterisation of thymine auxotrophy in Escherichia coli isolated from a patient with recurrent bloodstream infection" @default.
• W4284965210 cites W1802421655 @default.
• W4284965210 cites W1940800772 @default.
• W4284965210 cites W1951861416 @default.
• W4284965210 cites W1970254466 @default.
• W4284965210 cites W1997743456 @default.
• W4284965210 cites W2031611770 @default.
• W4284965210 cites W2045586398 @default.
• W4284965210 cites W2055046588 @default.
• W4284965210 cites W2088960594 @default.
• W4284965210 cites W2120902911 @default.
• W4284965210 cites W2122673596 @default.
• W4284965210 cites W2126149051 @default.
• W4284965210 cites W2134454646 @default.
• W4284965210 cites W2139412554 @default.
• W4284965210 cites W2152640773 @default.
• W4284965210 cites W2152975700 @default.
• W4284965210 cites W2154858303 @default.
• W4284965210 cites W2168444466 @default.
• W4284965210 cites W2181020278 @default.
• W4284965210 cites W2769633444 @default.
• W4284965210 cites W2799524357 @default.
• W4284965210 cites W2946162013 @default.
• W4284965210 cites W2995570923 @default.
• W4284965210 cites W3008287718 @default.
• W4284965210 cites W3036401848 @default.
• W4284965210 doi "https://doi.org/10.1371/journal.pone.0270256" @default.
• W4284965210 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35802671" @default.
• W4284965210 hasPublicationYear "2022" @default.
• W4284965210 type Work @default.
• W4284965210 citedByCount "2" @default.
• W4284965210 countsByYear W42849652102022 @default.
• W4284965210 crossrefType "journal-article" @default.
• W4284965210 hasAuthorship W4284965210A5003446319 @default.
• W4284965210 hasAuthorship W4284965210A5011018087 @default.
• W4284965210 hasAuthorship W4284965210A5030869812 @default.
• W4284965210 hasAuthorship W4284965210A5063386849 @default.
• W4284965210 hasAuthorship W4284965210A5079938824 @default.
• W4284965210 hasAuthorship W4284965210A5086464504 @default.
• W4284965210 hasAuthorship W4284965210A5087353619 @default.
• W4284965210 hasBestOaLocation W42849652101 @default.
• W4284965210 hasConcept C104317684 @default.
• W4284965210 hasConcept C20950039 @default.
• W4284965210 hasConcept C2776858809 @default.
• W4284965210 hasConcept C2778660310 @default.
• W4284965210 hasConcept C2779489039 @default.
• W4284965210 hasConcept C2779895986 @default.
• W4284965210 hasConcept C523546767 @default.
• W4284965210 hasConcept C54355233 @default.
• W4284965210 hasConcept C547475151 @default.
• W4284965210 hasConcept C552990157 @default.
• W4284965210 hasConcept C86803240 @default.
• W4284965210 hasConcept C89423630 @default.
• W4284965210 hasConceptScore W4284965210C104317684 @default.
• W4284965210 hasConceptScore W4284965210C20950039 @default.
• W4284965210 hasConceptScore W4284965210C2776858809 @default.
• W4284965210 hasConceptScore W4284965210C2778660310 @default.
• W4284965210 hasConceptScore W4284965210C2779489039 @default.
• W4284965210 hasConceptScore W4284965210C2779895986 @default.
• W4284965210 hasConceptScore W4284965210C523546767 @default.
• W4284965210 hasConceptScore W4284965210C54355233 @default.
• W4284965210 hasConceptScore W4284965210C547475151 @default.
• W4284965210 hasConceptScore W4284965210C552990157 @default.
• W4284965210 hasConceptScore W4284965210C86803240 @default.
• W4284965210 hasConceptScore W4284965210C89423630 @default.
• W4284965210 hasIssue "7" @default.
• W4284965210 hasLocation W42849652101 @default.
• W4284965210 hasLocation W42849652102 @default.
• W4284965210 hasLocation W42849652103 @default.
• W4284965210 hasLocation W42849652104 @default.
• W4284965210 hasLocation W42849652105 @default.
• W4284965210 hasOpenAccess W4284965210 @default.
• W4284965210 hasPrimaryLocation W42849652101 @default.
• W4284965210 hasRelatedWork W1983271939 @default.
• W4284965210 hasRelatedWork W2087522050 @default.
• W4284965210 hasRelatedWork W2103274496 @default.
• W4284965210 hasRelatedWork W2112649185 @default.
• W4284965210 hasRelatedWork W2123345500 @default.
• W4284965210 hasRelatedWork W2259078774 @default.
• W4284965210 hasRelatedWork W2313387403 @default.
• W4284965210 hasRelatedWork W2383245328 @default.
• W4284965210 hasRelatedWork W2409287164 @default.
• W4284965210 hasRelatedWork W4239219832 @default.
• W4284965210 hasVolume "17" @default.
• W4284965210 isParatext "false" @default.
• W4284965210 isRetracted "false" @default.
• W4284965210 workType "article" @default.