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• W4284990211 abstract "Abstract Meiotic maturation and cumulus expansion are essential for the generation of a developmentally competent gamete, and both processes can be recapitulated in vitro. We used a closed time-lapse incubator (EmbryoScope+™) to establish morphokinetic parameters of meiotic progression and cumulus expansion in mice and correlated these outcomes with egg ploidy. The average time to germinal vesicle breakdown (GVBD), time to first polar body extrusion (PBE), and duration of meiosis I were 0.91 ± 0.01, 8.82 ± 0.06, and 7.93 ± 0.06 h, respectively. The overall rate of cumulus layer expansion was 0.091 ± 0.002 μm/min, and the velocity of expansion peaked during the first 8 h of in vitro maturation (IVM) and then slowed. IVM of oocytes exposed to Nocodazole, a microtubule disrupting agent, and cumulus oocyte complexes (COCs) to 4-methylumbelliferone, a hyaluronan synthesis inhibitor, resulted in a dose-dependent perturbation of morphokinetics, thereby validating the system. The incidence of euploidy following IVM was &gt;90% for both denuded oocytes and intact COCs. No differences were observed between euploid and aneuploid eggs with respect to time to GVBD (0.90 ± 0.22 vs. 0.97 ± 0.19 h), time to PBE (8.89 ± 0.98 vs. 9.10 ± 1.42 h), duration of meiosis I (8.01 ± 0.91 vs. 8.13 ± 1.38 h), and overall rate and kinetics of cumulus expansion (0.089 ± 0.02 vs 0.088 ± 0.03 μm/min) (P &gt; 0.05). These morphokinetic parameters provide novel quantitative and non-invasive metrics for the evaluation of meiotic maturation and cumulus expansion and will enable screening compounds that modulate these processes." @default.
• W4284990211 created "2022-07-11" @default.
• W4284990211 creator A5032413676 @default.
• W4284990211 creator A5047358815 @default.
• W4284990211 creator A5049907659 @default.
• W4284990211 creator A5065873449 @default.
• W4284990211 creator A5082020231 @default.
• W4284990211 date "2022-07-08" @default.
• W4284990211 modified "2023-10-14" @default.
• W4284990211 title "Quantitative morphokinetic parameters identify novel dynamics of oocyte meiotic maturation and cumulus expansion" @default.
• W4284990211 cites W120073335 @default.
• W4284990211 cites W1845926721 @default.
• W4284990211 cites W1892345804 @default.
• W4284990211 cites W1968374826 @default.
• W4284990211 cites W1974916178 @default.
• W4284990211 cites W1976919632 @default.
• W4284990211 cites W1979707418 @default.
• W4284990211 cites W1993444917 @default.
• W4284990211 cites W1994567108 @default.
• W4284990211 cites W1999337849 @default.
• W4284990211 cites W1999600600 @default.
• W4284990211 cites W2016276264 @default.
• W4284990211 cites W2019108353 @default.
• W4284990211 cites W2029178312 @default.
• W4284990211 cites W2029394548 @default.
• W4284990211 cites W2035240370 @default.
• W4284990211 cites W2037890172 @default.
• W4284990211 cites W2057694188 @default.
• W4284990211 cites W2058732512 @default.
• W4284990211 cites W2061428016 @default.
• W4284990211 cites W2062330395 @default.
• W4284990211 cites W2071770630 @default.
• W4284990211 cites W2072708127 @default.
• W4284990211 cites W2084134136 @default.
• W4284990211 cites W2085505753 @default.
• W4284990211 cites W2086358586 @default.
• W4284990211 cites W2090125861 @default.
• W4284990211 cites W2090556419 @default.
• W4284990211 cites W2092739399 @default.
• W4284990211 cites W2096537301 @default.
• W4284990211 cites W2099544293 @default.
• W4284990211 cites W2100512407 @default.
• W4284990211 cites W2110912407 @default.
• W4284990211 cites W2113621013 @default.
• W4284990211 cites W2116761578 @default.
• W4284990211 cites W2122773562 @default.
• W4284990211 cites W2124381929 @default.
• W4284990211 cites W2126722856 @default.
• W4284990211 cites W2132020112 @default.
• W4284990211 cites W2135127763 @default.
• W4284990211 cites W2136935806 @default.
• W4284990211 cites W2137317211 @default.
• W4284990211 cites W2141656185 @default.
• W4284990211 cites W2142206986 @default.
• W4284990211 cites W2145431959 @default.
• W4284990211 cites W2149237119 @default.
• W4284990211 cites W2157077875 @default.
• W4284990211 cites W2158137024 @default.
• W4284990211 cites W2166516637 @default.
• W4284990211 cites W2289928043 @default.
• W4284990211 cites W2340936863 @default.
• W4284990211 cites W2401943237 @default.
• W4284990211 cites W2461066519 @default.
• W4284990211 cites W2514853184 @default.
• W4284990211 cites W2529392187 @default.
• W4284990211 cites W2588024230 @default.
• W4284990211 cites W2598106693 @default.
• W4284990211 cites W2625620806 @default.
• W4284990211 cites W2744305255 @default.
• W4284990211 cites W2744366548 @default.
• W4284990211 cites W2756042162 @default.
• W4284990211 cites W2764279830 @default.
• W4284990211 cites W2789822652 @default.
• W4284990211 cites W2802045231 @default.
• W4284990211 cites W2804653351 @default.
• W4284990211 cites W2872709060 @default.
• W4284990211 cites W2915005347 @default.
• W4284990211 cites W2936815201 @default.
• W4284990211 cites W2953489573 @default.
• W4284990211 cites W2966429881 @default.
• W4284990211 cites W2969755598 @default.
• W4284990211 cites W2988755269 @default.
• W4284990211 cites W3011342905 @default.
• W4284990211 cites W3024252005 @default.
• W4284990211 cites W3024753825 @default.
• W4284990211 cites W3081133467 @default.
• W4284990211 cites W3083879023 @default.
• W4284990211 cites W3093327874 @default.
• W4284990211 cites W3112904566 @default.
• W4284990211 cites W3116935034 @default.
• W4284990211 cites W3126600000 @default.
• W4284990211 cites W3139168703 @default.
• W4284990211 cites W3187483733 @default.
• W4284990211 cites W3193545945 @default.
• W4284990211 cites W3197162943 @default.
• W4284990211 cites W3198210930 @default.
• W4284990211 cites W3207410931 @default.
• W4284990211 cites W4213435613 @default.
• W4284990211 cites W4236478801 @default.
• W4284990211 cites W4239595888 @default.

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