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- W4284990911 abstract "Abstract Aims Actinobacteria are known to produce extracellular enzymes including DyPs. We set out to identify and characterize novel peroxidases from Streptomyces chartreusis NRRL 3882, because S. chartreusis belongs to the small group of actinobacteria with three different DyPs. Methods and Results The genome of the actinomycete S. chartreusis NRRL 3882 was mined for novel DyP-type peroxidases. Three genes encoding for DyP-type peroxidases were cloned and overexpressed in Escherichia coli. Subsequent characterization of the recombinant proteins included examination of operating conditions such as pH, temperature and H2O2 concentrations, as well as substrate spectrum. Despite their high sequence similarity, the enzymes named SCDYP1-SCDYP3 presented distinct preferences regarding their operating conditions. They showed great divergence in H2O2 tolerance and stability, with SCDYP2 being most active at concentrations above 50 mmol l−1. Moreover, SCDYP1 and SCDYP3 preferred acidic pH (typical for DyP-type peroxidases), whereas SCDYP2 was most active at pH 8. Conclusions Regarding the function of DyPs in nature, these results suggest that availability of different DyP variants with complementary activity profiles in one organism might convey evolutionary benefits. Significance and Impact of the Study DyP-type peroxidases are able to degrade xenobiotic compounds and thus can be applied in biocatalysis and bioremediation. However, the native function of DyPs and the benefits for their producers largely remain to be elucidated." @default.
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- W4284990911 date "2022-10-01" @default.
- W4284990911 modified "2023-10-15" @default.
- W4284990911 title "Characterization of three novel DyP-type peroxidases from Streptomyces chartreusis NRRL 3882" @default.
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- W4284990911 doi "https://doi.org/10.1111/jam.15707" @default.
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