FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4285010291> ?p ?o ?g. }

• W4285010291 abstract "Cocoonase is a serine protease present in sericigenous insects and majorly involved in dissolving of sericin protein allowing moth to escape. Cocoon structure is made up of sericin protein which holds fibroin filaments together. Cocoonase enzyme hydrolyzes sericin protein without harming the fibroin. However, until date, no detailed characterization of cocoonase enzyme and its presence in wild silk moth Antheraea mylitta has been carried out. Therefore, current study aimed for detailed characterization of amplified cocoonase enzyme, secondary and tertiary structure prediction, sequence and structural alignment, phylogenetic analysis, and computational validation. Several computational tools such as ProtParam, Iterative Threading Assembly Refinement (I-TASSER), PROCHECK, SAVES v6.0, TM-align, Molecular Evolutionary Genetics Analysis (MEGA) X, and Figtree were employed for characterization of cocoonase protein.The present study elucidates about the isolation of RNA, cDNA preparation, PCR amplification, and in silico characterization of cocoonase from Antheraea mylitta. Here, total RNA was isolated from head region of A. mylitta, and gene-specific primers were designed using Primer3 followed by PCR-based amplification and sequencing. The newly constructed 377-bp length sequence of cocoonase was subjected to in silico characterization. In silico study of A. mylitta cocoonase showed 26% similarity to A. pernyi strain Qing-6 cocoonase using Blastp and belongs to member of chymotrypsin-like serine protease superfamily. From phylogenetic study, it was found that A. mylitta cocoonase sequence is closely related to A. pernyi cocoonase sequence.The present study revealed about the detailed in silico characterization of cocoonase gene and encoded protein obtained from A. mylitta head region. The results obtained infer the presence of cocoonase enzyme in the wild silkworm A. mylitta and can be used for cocoon degumming which will be a valuable and cost-effective strategy in silk industry." @default.
• W4285010291 created "2022-07-12" @default.
• W4285010291 creator A5013456832 @default.
• W4285010291 creator A5052819001 @default.
• W4285010291 date "2022-07-11" @default.
• W4285010291 modified "2023-10-14" @default.
• W4285010291 title "In silico structural and functional characterization of Antheraea mylitta cocoonase" @default.
• W4285010291 cites W1603231983 @default.
• W4285010291 cites W1632131612 @default.
• W4285010291 cites W1913981180 @default.
• W4285010291 cites W1963550288 @default.
• W4285010291 cites W1975459052 @default.
• W4285010291 cites W1977800236 @default.
• W4285010291 cites W1985697238 @default.
• W4285010291 cites W1986191025 @default.
• W4285010291 cites W1987134040 @default.
• W4285010291 cites W1992192767 @default.
• W4285010291 cites W1999299228 @default.
• W4285010291 cites W2000550628 @default.
• W4285010291 cites W2004991228 @default.
• W4285010291 cites W2006181694 @default.
• W4285010291 cites W2008812269 @default.
• W4285010291 cites W2010881677 @default.
• W4285010291 cites W2011983053 @default.
• W4285010291 cites W2015921978 @default.
• W4285010291 cites W2015928352 @default.
• W4285010291 cites W2037312364 @default.
• W4285010291 cites W2037993016 @default.
• W4285010291 cites W2068631308 @default.
• W4285010291 cites W2074037462 @default.
• W4285010291 cites W2074986801 @default.
• W4285010291 cites W2079962051 @default.
• W4285010291 cites W2081367528 @default.
• W4285010291 cites W2094201044 @default.
• W4285010291 cites W2094931538 @default.
• W4285010291 cites W2097632784 @default.
• W4285010291 cites W2097856811 @default.
• W4285010291 cites W2101521215 @default.
• W4285010291 cites W2102245393 @default.
• W4285010291 cites W2107492558 @default.
• W4285010291 cites W2108787198 @default.
• W4285010291 cites W2118340207 @default.
• W4285010291 cites W2122968926 @default.
• W4285010291 cites W2132744530 @default.
• W4285010291 cites W2137974866 @default.
• W4285010291 cites W2140538197 @default.
• W4285010291 cites W2153544371 @default.
• W4285010291 cites W2155201844 @default.
• W4285010291 cites W2157165549 @default.
• W4285010291 cites W2163900914 @default.
• W4285010291 cites W2266510186 @default.
• W4285010291 cites W2282013022 @default.
• W4285010291 cites W2312798167 @default.
• W4285010291 cites W2326356507 @default.
• W4285010291 cites W2515982926 @default.
• W4285010291 cites W2611792444 @default.
• W4285010291 cites W2759820149 @default.
• W4285010291 cites W2766352633 @default.
• W4285010291 cites W2782157537 @default.
• W4285010291 cites W2884610933 @default.
• W4285010291 cites W2910485623 @default.
• W4285010291 cites W2966045382 @default.
• W4285010291 cites W2984761660 @default.
• W4285010291 cites W3009822181 @default.
• W4285010291 cites W3083465006 @default.
• W4285010291 cites W3088993695 @default.
• W4285010291 cites W3093868212 @default.
• W4285010291 cites W3132162994 @default.
• W4285010291 cites W4241454816 @default.
• W4285010291 cites W4244736178 @default.
• W4285010291 doi "https://doi.org/10.1186/s43141-022-00367-8" @default.
• W4285010291 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35816268" @default.
• W4285010291 hasPublicationYear "2022" @default.
• W4285010291 type Work @default.
• W4285010291 citedByCount "1" @default.
• W4285010291 countsByYear W42850102912023 @default.
• W4285010291 crossrefType "journal-article" @default.
• W4285010291 hasAuthorship W4285010291A5013456832 @default.
• W4285010291 hasAuthorship W4285010291A5052819001 @default.
• W4285010291 hasBestOaLocation W42850102911 @default.
• W4285010291 hasConcept C10010492 @default.
• W4285010291 hasConcept C104317684 @default.
• W4285010291 hasConcept C167625842 @default.
• W4285010291 hasConcept C181199279 @default.
• W4285010291 hasConcept C2775905019 @default.
• W4285010291 hasConcept C2776714187 @default.
• W4285010291 hasConcept C2777807008 @default.
• W4285010291 hasConcept C45484198 @default.
• W4285010291 hasConcept C54355233 @default.
• W4285010291 hasConcept C55493867 @default.
• W4285010291 hasConcept C61053724 @default.
• W4285010291 hasConcept C70721500 @default.
• W4285010291 hasConcept C86803240 @default.
• W4285010291 hasConcept C88031987 @default.
• W4285010291 hasConceptScore W4285010291C10010492 @default.
• W4285010291 hasConceptScore W4285010291C104317684 @default.
• W4285010291 hasConceptScore W4285010291C167625842 @default.
• W4285010291 hasConceptScore W4285010291C181199279 @default.
• W4285010291 hasConceptScore W4285010291C2775905019 @default.
• W4285010291 hasConceptScore W4285010291C2776714187 @default.

• next