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- W4285010908 abstract "The human MUS81-EME1&2 complexes are structure-selective endonucleases that play important roles in DNA damage repair. Here, we describe a protocol to determine the endonuclease activities of MUS81-EME1&2 complexes toward various DNA structures. We co-express MUS81 with EME1 or EME2 and purify the complexes with high purity, and determine their activities on the cleavages of 3' flaps, 5' flaps, nicked double-stranded DNAs, and Holliday junctions. This protocol can also be used for the determination of substrate preferences of other structure-selective endonucleases. For complete details on the use and execution of this protocol, please refer to Hua et al. (2022)." @default.
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- W4285010908 date "2022-09-01" @default.
- W4285010908 modified "2023-10-04" @default.
- W4285010908 title "A protocol to determine the activities of human MUS81-EME1&2 endonucleases" @default.
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- W4285010908 doi "https://doi.org/10.1016/j.xpro.2022.101528" @default.
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