FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4285087657> ?p ?o ?g. }

• W4285087657 endingPage "1695" @default.
• W4285087657 startingPage "1682" @default.
• W4285087657 abstract "Abstract Xyloglucan (XG) is an abundant polysaccharide in plant cell walls and some seeds and requires at least four different enzymes for complete depolymerization. Recombinant xyloglucanase from Aspergillus niveus (XegA), α ‐xylosidase from Escherichia coli (YicI), β ‐galactosidase from Hypocrea jecorina (Bga1) and β ‐glucosidase from H. jecorina (Bgl1) were covalently immobilized individually and in combination on chitosan‐coated ferromagnetic iron oxide nanoparticles functionalized with glutaraldehyde. All immobilized enzymes presented reduced specific catalytic activity, where immobilized YicI, XegA, Bga1 and Bgl1 retained 14.9, 76.9, 29.5 and 6.6% activity as compared with the free enzymes, respectively. Immobilized and free enzymes presented similar optimum catalytic pH and optimum catalytic temperatures differed by ±10°C between the free and immobilized enzymes. Bga1 and Bgl1 presented decreased maximum catalytic temperatures ( T opt ), while YicI presented an increased T opt . The T opt of XegA remained unaltered. Mass spectrometry confirmed that nanoparticles carrying all four co‐immobilized enzymes degraded XG to glucose, galactose and xylose, and higher proportions of co‐immobilized Bgl1 and XegA resulted in higher XG saccharification. Although levels of Bgl1 activity were limiting, five re‐use cycles of the co‐immobilized enzymes were demonstrated, providing proof‐of‐principle for the use of a four‐component multienzyme nanoparticle in the breakdown of a complex polysaccharide. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd." @default.
• W4285087657 created "2022-07-14" @default.
• W4285087657 creator A5023893154 @default.
• W4285087657 creator A5028069486 @default.
• W4285087657 creator A5060791179 @default.
• W4285087657 creator A5061697567 @default.
• W4285087657 date "2022-08-02" @default.
• W4285087657 modified "2023-10-15" @default.
• W4285087657 title "Co‐immobilization of multiple enzymes on ferromagnetic nanoparticles for the depolymerization of xyloglucan" @default.
• W4285087657 cites W1975330570 @default.
• W4285087657 cites W1982853929 @default.
• W4285087657 cites W2009674410 @default.
• W4285087657 cites W2012484827 @default.
• W4285087657 cites W2015056235 @default.
• W4285087657 cites W2019233287 @default.
• W4285087657 cites W2035814116 @default.
• W4285087657 cites W2037891557 @default.
• W4285087657 cites W2043451183 @default.
• W4285087657 cites W2078308695 @default.
• W4285087657 cites W2079013207 @default.
• W4285087657 cites W2079173335 @default.
• W4285087657 cites W2094463994 @default.
• W4285087657 cites W2100837269 @default.
• W4285087657 cites W2122881788 @default.
• W4285087657 cites W2134630855 @default.
• W4285087657 cites W2142521359 @default.
• W4285087657 cites W2149539286 @default.
• W4285087657 cites W2152621767 @default.
• W4285087657 cites W2168983709 @default.
• W4285087657 cites W2281965470 @default.
• W4285087657 cites W243303449 @default.
• W4285087657 cites W2562647913 @default.
• W4285087657 cites W2771047142 @default.
• W4285087657 cites W2793690010 @default.
• W4285087657 cites W2797024879 @default.
• W4285087657 cites W2804661079 @default.
• W4285087657 cites W2882995781 @default.
• W4285087657 cites W2883695321 @default.
• W4285087657 cites W2929343448 @default.
• W4285087657 cites W2977808409 @default.
• W4285087657 cites W2978940530 @default.
• W4285087657 cites W2983690960 @default.
• W4285087657 cites W3034699637 @default.
• W4285087657 cites W3040779369 @default.
• W4285087657 cites W4220934446 @default.
• W4285087657 cites W630476006 @default.
• W4285087657 doi "https://doi.org/10.1002/bbb.2407" @default.
• W4285087657 hasPublicationYear "2022" @default.
• W4285087657 type Work @default.
• W4285087657 citedByCount "2" @default.
• W4285087657 countsByYear W42850876572022 @default.
• W4285087657 countsByYear W42850876572023 @default.
• W4285087657 crossrefType "journal-article" @default.
• W4285087657 hasAuthorship W4285087657A5023893154 @default.
• W4285087657 hasAuthorship W4285087657A5028069486 @default.
• W4285087657 hasAuthorship W4285087657A5060791179 @default.
• W4285087657 hasAuthorship W4285087657A5061697567 @default.
• W4285087657 hasConcept C100544194 @default.
• W4285087657 hasConcept C100817775 @default.
• W4285087657 hasConcept C104317684 @default.
• W4285087657 hasConcept C154881586 @default.
• W4285087657 hasConcept C155672457 @default.
• W4285087657 hasConcept C161790260 @default.
• W4285087657 hasConcept C171250308 @default.
• W4285087657 hasConcept C178790620 @default.
• W4285087657 hasConcept C181199279 @default.
• W4285087657 hasConcept C185592680 @default.
• W4285087657 hasConcept C191503008 @default.
• W4285087657 hasConcept C192562407 @default.
• W4285087657 hasConcept C2777529624 @default.
• W4285087657 hasConcept C2779741953 @default.
• W4285087657 hasConcept C2780294150 @default.
• W4285087657 hasConcept C33633552 @default.
• W4285087657 hasConcept C43617362 @default.
• W4285087657 hasConcept C49852136 @default.
• W4285087657 hasConcept C547475151 @default.
• W4285087657 hasConcept C55493867 @default.
• W4285087657 hasConcept C94412978 @default.
• W4285087657 hasConceptScore W4285087657C100544194 @default.
• W4285087657 hasConceptScore W4285087657C100817775 @default.
• W4285087657 hasConceptScore W4285087657C104317684 @default.
• W4285087657 hasConceptScore W4285087657C154881586 @default.
• W4285087657 hasConceptScore W4285087657C155672457 @default.
• W4285087657 hasConceptScore W4285087657C161790260 @default.
• W4285087657 hasConceptScore W4285087657C171250308 @default.
• W4285087657 hasConceptScore W4285087657C178790620 @default.
• W4285087657 hasConceptScore W4285087657C181199279 @default.
• W4285087657 hasConceptScore W4285087657C185592680 @default.
• W4285087657 hasConceptScore W4285087657C191503008 @default.
• W4285087657 hasConceptScore W4285087657C192562407 @default.
• W4285087657 hasConceptScore W4285087657C2777529624 @default.
• W4285087657 hasConceptScore W4285087657C2779741953 @default.
• W4285087657 hasConceptScore W4285087657C2780294150 @default.
• W4285087657 hasConceptScore W4285087657C33633552 @default.
• W4285087657 hasConceptScore W4285087657C43617362 @default.
• W4285087657 hasConceptScore W4285087657C49852136 @default.
• W4285087657 hasConceptScore W4285087657C547475151 @default.
• W4285087657 hasConceptScore W4285087657C55493867 @default.

• next