FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4285122246> ?p ?o ?g. }

• W4285122246 endingPage "117693432211082" @default.
• W4285122246 startingPage "117693432211082" @default.
• W4285122246 abstract "Introduction: In an effort to combat SARS-CoV-2 through multi-subunit vaccine design, during studies using whole genome and immunome, ORF10, located at the 3′ end of the genome, displayed unique features. It showed no homology to any known protein in other organisms, including SARS-CoV. It was observed that its nucleotide sequence is 100% identical in the SARS-CoV-2 genomes sourced worldwide, even in the recent-most VoCs and VoIs of B.1.1.529 (Omicron), B.1.617 (Delta), B.1.1.7 (Alpha), B.1.351 (Beta), and P.1 (Gamma) lineages, implicating its constant nature throughout the evolution of deadly variants. Aim: The structure and function of SARS-CoV-2 ORF10 and the role it may play in the viral evolution is yet to be understood clearly. The aim of this study is to predict its structure, function, and understand evolutionary dynamics on the basis of mutations and likely heightened immune responses in the immunopathogenesis of this deadly virus. Methods: Sequence analysis, ab-initio structure modeling and an understanding of the impact of likely substitutions in key regions of protein was carried out. Analyses of viral T cell epitopes and primary anchor residue mutations was done to understand the role it may play in the evolution as a molecule with likely enhanced immune response and consequent immunopathogenesis. Results: Few amino acid substitution mutations are observed, most probably due to the ribosomal frameshifting, and these mutations may not be detrimental to its functioning. As ORF10 is observed to be an expressed protein, ab-initio structure modeling shows that it comprises mainly an α-helical region and maybe an ER-targeted membrane mini-protein. Analyzing the whole proteome, it is observed that ORF10 presents amongst the highest number of likely promiscuous and immunogenic CTL epitopes, specifically 11 out of 30 promiscuous ones and 9 out of these 11, immunogenic CTL epitopes. Reactive T cells to these epitopes have been uncovered in independent studies. Majority of these epitopes are located on the α-helix region of its structure, and the substitution mutations of primary anchor residues in these epitopes do not affect immunogenicity. Its conserved nucleotide sequence throughout the evolution and diversification of virus into several variants is a puzzle yet to be solved. Conclusions: On the basis of its sequence, structure, and epitope mapping, it is concluded that it may function like those mini-proteins used to boost immune responses in medical applications. Due to the complete nucleotide sequence conservation even a few years after SARS-CoV-2 genome was first sequenced, it poses a unique puzzle to be solved, in view of the evolutionary dynamics of variants emerging in the populations worldwide." @default.
• W4285122246 created "2022-07-14" @default.
• W4285122246 creator A5076376019 @default.
• W4285122246 date "2022-01-01" @default.
• W4285122246 modified "2023-09-25" @default.
• W4285122246 title "Computational Structural and Functional Analyses of ORF10 in Novel Coronavirus SARS-CoV-2 Variants to Understand Evolutionary Dynamics" @default.
• W4285122246 cites W1483633199 @default.
• W4285122246 cites W1965669216 @default.
• W4285122246 cites W1970594649 @default.
• W4285122246 cites W1973980688 @default.
• W4285122246 cites W1997094385 @default.
• W4285122246 cites W2000455214 @default.
• W4285122246 cites W2005380527 @default.
• W4285122246 cites W2014537819 @default.
• W4285122246 cites W2062663912 @default.
• W4285122246 cites W2089257957 @default.
• W4285122246 cites W2100424218 @default.
• W4285122246 cites W2100494857 @default.
• W4285122246 cites W2108446353 @default.
• W4285122246 cites W2110694683 @default.
• W4285122246 cites W2124088653 @default.
• W4285122246 cites W2142269152 @default.
• W4285122246 cites W2152770371 @default.
• W4285122246 cites W2154479185 @default.
• W4285122246 cites W2167597940 @default.
• W4285122246 cites W2170060741 @default.
• W4285122246 cites W2406768133 @default.
• W4285122246 cites W2756455857 @default.
• W4285122246 cites W2884538216 @default.
• W4285122246 cites W2953725799 @default.
• W4285122246 cites W3011017798 @default.
• W4285122246 cites W3017680297 @default.
• W4285122246 cites W3022285953 @default.
• W4285122246 cites W3084071750 @default.
• W4285122246 cites W3084914618 @default.
• W4285122246 cites W3086164336 @default.
• W4285122246 cites W3089759643 @default.
• W4285122246 cites W3092226560 @default.
• W4285122246 cites W3093824751 @default.
• W4285122246 cites W3103140952 @default.
• W4285122246 cites W3108728530 @default.
• W4285122246 cites W3111804069 @default.
• W4285122246 cites W3149637542 @default.
• W4285122246 cites W3161503920 @default.
• W4285122246 cites W3172192134 @default.
• W4285122246 cites W4205700484 @default.
• W4285122246 cites W4211196031 @default.
• W4285122246 cites W4225864665 @default.
• W4285122246 cites W4236833372 @default.
• W4285122246 cites W4254454535 @default.
• W4285122246 doi "https://doi.org/10.1177/11769343221108218" @default.
• W4285122246 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35909986" @default.
• W4285122246 hasPublicationYear "2022" @default.
• W4285122246 type Work @default.
• W4285122246 citedByCount "1" @default.
• W4285122246 countsByYear W42851222462023 @default.
• W4285122246 crossrefType "journal-article" @default.
• W4285122246 hasAuthorship W4285122246A5076376019 @default.
• W4285122246 hasBestOaLocation W42851222461 @default.
• W4285122246 hasConcept C104317684 @default.
• W4285122246 hasConcept C141231307 @default.
• W4285122246 hasConcept C142724271 @default.
• W4285122246 hasConcept C169627665 @default.
• W4285122246 hasConcept C181199279 @default.
• W4285122246 hasConcept C2522874641 @default.
• W4285122246 hasConcept C2777648638 @default.
• W4285122246 hasConcept C2779134260 @default.
• W4285122246 hasConcept C2780879997 @default.
• W4285122246 hasConcept C3007834351 @default.
• W4285122246 hasConcept C3008058167 @default.
• W4285122246 hasConcept C501734568 @default.
• W4285122246 hasConcept C524204448 @default.
• W4285122246 hasConcept C54355233 @default.
• W4285122246 hasConcept C55493867 @default.
• W4285122246 hasConcept C70721500 @default.
• W4285122246 hasConcept C71924100 @default.
• W4285122246 hasConcept C86803240 @default.
• W4285122246 hasConceptScore W4285122246C104317684 @default.
• W4285122246 hasConceptScore W4285122246C141231307 @default.
• W4285122246 hasConceptScore W4285122246C142724271 @default.
• W4285122246 hasConceptScore W4285122246C169627665 @default.
• W4285122246 hasConceptScore W4285122246C181199279 @default.
• W4285122246 hasConceptScore W4285122246C2522874641 @default.
• W4285122246 hasConceptScore W4285122246C2777648638 @default.
• W4285122246 hasConceptScore W4285122246C2779134260 @default.
• W4285122246 hasConceptScore W4285122246C2780879997 @default.
• W4285122246 hasConceptScore W4285122246C3007834351 @default.
• W4285122246 hasConceptScore W4285122246C3008058167 @default.
• W4285122246 hasConceptScore W4285122246C501734568 @default.
• W4285122246 hasConceptScore W4285122246C524204448 @default.
• W4285122246 hasConceptScore W4285122246C54355233 @default.
• W4285122246 hasConceptScore W4285122246C55493867 @default.
• W4285122246 hasConceptScore W4285122246C70721500 @default.
• W4285122246 hasConceptScore W4285122246C71924100 @default.
• W4285122246 hasConceptScore W4285122246C86803240 @default.
• W4285122246 hasLocation W42851222461 @default.
• W4285122246 hasLocation W42851222462 @default.
• W4285122246 hasLocation W42851222463 @default.

• next