FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4285140261> ?p ?o ?g. }

• W4285140261 endingPage "780" @default.
• W4285140261 startingPage "756" @default.
• W4285140261 abstract "In plants, Dof transcription factors are involved in regulating the expression of a series of genes related to N uptake and utilization. Therefore, the present study investigated how DNA-binding with one finger (Dof) genes are expressed in response to nitrogen (N) form and concentration to clarify the role of Dof genes and their functions in promoting N assimilation and utilization in poplar. The basic characteristics and expression patterns of Dof genes in poplar were analyzed by the use of bioinformatics methods. Dof genes expressed in response to N were screened, after which the related genes were cloned and transformed into Arabidopsis thaliana; the physiological indexes and the expression of related genes were subsequently determined. The function of Dof genes was then verified in Arabidopsis thaliana plants grown in the presence of different N forms and concentrations. Forty-four Dof genes were identified, most of which were expressed in the roots and young leaves, and some of the Dof genes were expressed under ammonia- and nitrate-N treatments. Three genes related to N induction were cloned, their proteins were found to localize in the nucleus, and PnDof30 was successfully transformed into Arabidopsis thaliana for functional verification. On comparing Arabidopsis thaliana with WT Arabidopsis thaliana plants, Arabidopsis thaliana plants overexpressing the Dof gene grew better under low N levels; the contents of soluble proteins and chlorophyll significantly increased, while the soluble sugar content significantly decreased. The expressions of several AMT, NRT, and GS genes were upregulated, while the expressions of several others were downregulated, and the expression of PEPC and PK genes significantly increased. In addition, the activity of PEPC, PK, GS, and NR enzymes significantly increased. The results showed that overexpression of PnDof30 significantly increased the level of carbon and N metabolism and improved the growth of transgenic Arabidopsis thaliana plants under low-N conditions. The study revealed the biological significance of poplar Dof transcription factors in N response and regulation of related downstream gene expression and provided some meaningful clues to explain the huge difference between poplar and Arabidopsis thaliana transformed by exogenous Dof gene, which could promote the comprehensive understanding of the molecular mechanism of efficient N uptake and utilization in trees." @default.
• W4285140261 created "2022-07-14" @default.
• W4285140261 creator A5058818052 @default.
• W4285140261 creator A5078535250 @default.
• W4285140261 creator A5033584840 @default.
• W4285140261 date "2022-01-01" @default.
• W4285140261 modified "2023-10-10" @default.
• W4285140261 title "Selection and functional identification of <i>Dof</i> genes expressed in response to nitrogen in <i>Populus simonii</i> × <i>Populus nigra</i>" @default.
• W4285140261 cites W1528951187 @default.
• W4285140261 cites W1536886974 @default.
• W4285140261 cites W1558047925 @default.
• W4285140261 cites W1971077045 @default.
• W4285140261 cites W1971587098 @default.
• W4285140261 cites W1974905780 @default.
• W4285140261 cites W1985447935 @default.
• W4285140261 cites W1989035557 @default.
• W4285140261 cites W1990187840 @default.
• W4285140261 cites W2001813373 @default.
• W4285140261 cites W2008011582 @default.
• W4285140261 cites W2019727923 @default.
• W4285140261 cites W2027996848 @default.
• W4285140261 cites W2037098737 @default.
• W4285140261 cites W2037694688 @default.
• W4285140261 cites W2048046638 @default.
• W4285140261 cites W2051202492 @default.
• W4285140261 cites W2055421247 @default.
• W4285140261 cites W2055590933 @default.
• W4285140261 cites W2064111631 @default.
• W4285140261 cites W2069453639 @default.
• W4285140261 cites W2074213315 @default.
• W4285140261 cites W2076844958 @default.
• W4285140261 cites W2100760318 @default.
• W4285140261 cites W2107748736 @default.
• W4285140261 cites W2108614436 @default.
• W4285140261 cites W2108900585 @default.
• W4285140261 cites W2116122104 @default.
• W4285140261 cites W2118903288 @default.
• W4285140261 cites W2119205648 @default.
• W4285140261 cites W2125346198 @default.
• W4285140261 cites W2126383712 @default.
• W4285140261 cites W2127647979 @default.
• W4285140261 cites W2132632499 @default.
• W4285140261 cites W2133917457 @default.
• W4285140261 cites W2136090457 @default.
• W4285140261 cites W2137015675 @default.
• W4285140261 cites W2140624038 @default.
• W4285140261 cites W2141292707 @default.
• W4285140261 cites W2143869990 @default.
• W4285140261 cites W2153792785 @default.
• W4285140261 cites W2154062191 @default.
• W4285140261 cites W2157472617 @default.
• W4285140261 cites W2172225462 @default.
• W4285140261 cites W2208260168 @default.
• W4285140261 cites W2315047122 @default.
• W4285140261 cites W2414722825 @default.
• W4285140261 cites W2527832349 @default.
• W4285140261 cites W2562809785 @default.
• W4285140261 cites W2579882411 @default.
• W4285140261 cites W2604793757 @default.
• W4285140261 cites W2765577491 @default.
• W4285140261 cites W2791695912 @default.
• W4285140261 cites W2801055184 @default.
• W4285140261 cites W2806166872 @default.
• W4285140261 cites W2921817981 @default.
• W4285140261 cites W2944742501 @default.
• W4285140261 cites W3039575116 @default.
• W4285140261 cites W3143082915 @default.
• W4285140261 doi "https://doi.org/10.1515/biol-2022-0084" @default.
• W4285140261 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35891966" @default.
• W4285140261 hasPublicationYear "2022" @default.
• W4285140261 type Work @default.
• W4285140261 citedByCount "1" @default.
• W4285140261 crossrefType "journal-article" @default.
• W4285140261 hasAuthorship W4285140261A5033584840 @default.
• W4285140261 hasAuthorship W4285140261A5058818052 @default.
• W4285140261 hasAuthorship W4285140261A5078535250 @default.
• W4285140261 hasBestOaLocation W42851402611 @default.
• W4285140261 hasConcept C104317684 @default.
• W4285140261 hasConcept C143065580 @default.
• W4285140261 hasConcept C150194340 @default.
• W4285140261 hasConcept C2775988993 @default.
• W4285140261 hasConcept C2779491563 @default.
• W4285140261 hasConcept C54355233 @default.
• W4285140261 hasConcept C59822182 @default.
• W4285140261 hasConcept C86803240 @default.
• W4285140261 hasConceptScore W4285140261C104317684 @default.
• W4285140261 hasConceptScore W4285140261C143065580 @default.
• W4285140261 hasConceptScore W4285140261C150194340 @default.
• W4285140261 hasConceptScore W4285140261C2775988993 @default.
• W4285140261 hasConceptScore W4285140261C2779491563 @default.
• W4285140261 hasConceptScore W4285140261C54355233 @default.
• W4285140261 hasConceptScore W4285140261C59822182 @default.
• W4285140261 hasConceptScore W4285140261C86803240 @default.
• W4285140261 hasIssue "1" @default.
• W4285140261 hasLocation W42851402611 @default.
• W4285140261 hasLocation W42851402612 @default.
• W4285140261 hasLocation W42851402613 @default.
• W4285140261 hasLocation W42851402614 @default.

• next