Matches in SemOpenAlex for { <https://semopenalex.org/work/W4285156857> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W4285156857 abstract "Chromatin accessibility is a key determinant of gene expression that can be altered under different physiological and disease conditions. Skeletal muscle is made up of myofibers that are highly plastic and adaptive. Therefore, assessing the genome-wide chromatin state of myofibers under various conditions is very important to gain insight into the epigenetic state of myonuclei. The rigid nature of myofibers, as well as the low number of myonuclei that they contain, have rendered genome-wide studies with myofibers challenging. In recent years, ATAC-Seq from whole muscle and single nucleus ATAC-Seq have been performed. However, these techniques cannot distinguish between different fiber and cell types present in the muscle. In addition, due to the limited depth capacity obtained from single nucleus ATAC-Seq, an extensive comparative analysis cannot be performed. Here, we introduce a protocol where we combine the isolation of a single myofiber with OMNI ATAC-Seq. This protocol allows for genome-wide analysis of accessible chromatin regions of a selected single myofiber at a sufficient depth for comparative analysis under various physiological and disease conditions. This protocol can also allow for a specific myofiber to be selected, such as a regenerating myofiber. In the future, this protocol can help identify global changes in chromatin state under various conditions, as well as between different types of myofibers. Graphical abstract." @default.
- W4285156857 created "2022-07-14" @default.
- W4285156857 creator A5022580269 @default.
- W4285156857 creator A5081321256 @default.
- W4285156857 creator A5083363037 @default.
- W4285156857 date "2022-01-01" @default.
- W4285156857 modified "2023-10-01" @default.
- W4285156857 title "ATAC-Seq of a Single Myofiber from Mus musculus" @default.
- W4285156857 cites W1531821806 @default.
- W4285156857 cites W1552651901 @default.
- W4285156857 cites W1981831271 @default.
- W4285156857 cites W1990786422 @default.
- W4285156857 cites W2083892140 @default.
- W4285156857 cites W2105199683 @default.
- W4285156857 cites W2111148036 @default.
- W4285156857 cites W2750416916 @default.
- W4285156857 cites W2775715715 @default.
- W4285156857 cites W2951091094 @default.
- W4285156857 cites W2977345005 @default.
- W4285156857 cites W3092065379 @default.
- W4285156857 cites W3092475087 @default.
- W4285156857 cites W3112658031 @default.
- W4285156857 cites W4220780146 @default.
- W4285156857 doi "https://doi.org/10.21769/bioprotoc.4452" @default.
- W4285156857 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35990589" @default.
- W4285156857 hasPublicationYear "2022" @default.
- W4285156857 type Work @default.
- W4285156857 citedByCount "1" @default.
- W4285156857 countsByYear W42851568572023 @default.
- W4285156857 crossrefType "journal-article" @default.
- W4285156857 hasAuthorship W4285156857A5022580269 @default.
- W4285156857 hasAuthorship W4285156857A5081321256 @default.
- W4285156857 hasAuthorship W4285156857A5083363037 @default.
- W4285156857 hasBestOaLocation W42851568571 @default.
- W4285156857 hasConcept C104317684 @default.
- W4285156857 hasConcept C141231307 @default.
- W4285156857 hasConcept C142724271 @default.
- W4285156857 hasConcept C207200792 @default.
- W4285156857 hasConcept C2779134260 @default.
- W4285156857 hasConcept C2992644362 @default.
- W4285156857 hasConcept C41091548 @default.
- W4285156857 hasConcept C54355233 @default.
- W4285156857 hasConcept C70721500 @default.
- W4285156857 hasConcept C71924100 @default.
- W4285156857 hasConcept C83640560 @default.
- W4285156857 hasConcept C86803240 @default.
- W4285156857 hasConcept C95444343 @default.
- W4285156857 hasConceptScore W4285156857C104317684 @default.
- W4285156857 hasConceptScore W4285156857C141231307 @default.
- W4285156857 hasConceptScore W4285156857C142724271 @default.
- W4285156857 hasConceptScore W4285156857C207200792 @default.
- W4285156857 hasConceptScore W4285156857C2779134260 @default.
- W4285156857 hasConceptScore W4285156857C2992644362 @default.
- W4285156857 hasConceptScore W4285156857C41091548 @default.
- W4285156857 hasConceptScore W4285156857C54355233 @default.
- W4285156857 hasConceptScore W4285156857C70721500 @default.
- W4285156857 hasConceptScore W4285156857C71924100 @default.
- W4285156857 hasConceptScore W4285156857C83640560 @default.
- W4285156857 hasConceptScore W4285156857C86803240 @default.
- W4285156857 hasConceptScore W4285156857C95444343 @default.
- W4285156857 hasIssue "12" @default.
- W4285156857 hasLocation W42851568571 @default.
- W4285156857 hasLocation W42851568572 @default.
- W4285156857 hasLocation W42851568573 @default.
- W4285156857 hasOpenAccess W4285156857 @default.
- W4285156857 hasPrimaryLocation W42851568571 @default.
- W4285156857 hasRelatedWork W1603543036 @default.
- W4285156857 hasRelatedWork W2002128513 @default.
- W4285156857 hasRelatedWork W2036519228 @default.
- W4285156857 hasRelatedWork W2069184423 @default.
- W4285156857 hasRelatedWork W2109945001 @default.
- W4285156857 hasRelatedWork W2172833929 @default.
- W4285156857 hasRelatedWork W2887293316 @default.
- W4285156857 hasRelatedWork W3167830717 @default.
- W4285156857 hasRelatedWork W4285156857 @default.
- W4285156857 hasRelatedWork W2187207551 @default.
- W4285156857 hasVolume "12" @default.
- W4285156857 isParatext "false" @default.
- W4285156857 isRetracted "false" @default.
- W4285156857 workType "article" @default.