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- W4285193762 abstract "We describe here the overproduction and oriented membrane insertion of membrane protein inside intracellular vesicles named heterologous caveolae within E. coli. The method is described with BmrA, a multidrug efflux pump from Bacillus subtilis. BmrA is produced in these vesicles, thanks to the coexpression with the canine caveolin-1β, one of the two isoforms of caveolin-1. Enriched by sucrose gradient, the caveolae-containing fraction allows to probe the ATPase and Hoechst 33342 transport activities, the latter displaying a higher specific activity than the same without caveolin-1β." @default.
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- W4285193762 date "2022-01-01" @default.
- W4285193762 modified "2023-09-24" @default.
- W4285193762 title "Overexpression of the ABC Transporter BmrA Within Intracellular Caveolae in Escherichia coli" @default.
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- W4285193762 doi "https://doi.org/10.1007/978-1-0716-2368-8_5" @default.
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