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- W4285195008 abstract "Detection of pathogens in air, water, soil, or food is essential due to their infectious nature. Raman microspectroscopy has emerged as a potential diagnostic technique for detection of bacteria. Vibrational spectroscopic technique such as Raman spectroscopy is nondestructive in nature and probes the bond molecular vibrations to yield structural information. Biological systems such as cells, tissues, and bacteria are composed of biomolecules like lipids, proteins, carbohydrates, and nucleic acids. Therefore, this spectroscopic technique can provide information pertaining to the molecular structure of the components. Various Raman-based techniques such as surface-enhanced Raman spectroscopy (SERS), resonance Raman spectroscopy (RR), etc. have been used for sensitive and selective detection of pathogens. Employing suitable substrates for accommodating biological entities that also provide high sensitivity and highly reproducible SERS can be a state-of-the-art method for pathogen detection. Use of required wavelength for excitation and proper sampling with advanced data analytics approach has led to pathogen detection by RRS. In addition, combination of chemometric tools aids in discrimination and classification of samples which is important in clinical and field environment. This chapter discusses the use of Raman spectroscopy-based approaches for detection of pathogens. Further, critical steps such as sampling, importance of pre-processing and post-processing, and instrumental considerations have also been discussed." @default.
- W4285195008 created "2022-07-14" @default.
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- W4285195008 date "2022-01-01" @default.
- W4285195008 modified "2023-10-12" @default.
- W4285195008 title "Raman Spectroscopy Applications for Bacterial Detection: Potential and Challenges Towards Development of Field Deployable System" @default.
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- W4285195008 doi "https://doi.org/10.1007/978-981-16-4345-3_20" @default.
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