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- W4285198527 abstract "Abstract Caesalpinia sappan L. ( C . sappan L.), a traditional Chinese medicine, has been widely used to treat bruises and dysmenorrhea, performing pharmacological activities such as anti-inflammatory and anti-tumor. C . sappan L. has been reported to contain compounds such as protosappanins, brazilins, and homoisoflavones. In the pre-experiments, we discovered that there were many unstable components in the characteristic chromatogram of C . sappan L. Here, silica-gel column chromatography, high-speed counter-current chromatography, and preparative high-performance liquid chromatography were combined and applied to isolate the unstable components from alcohol extract of C . sappan L. The results showed that four unstable compounds were collected with the purity higher than 95.0%, characterized as episapponal, brazilin, sapponal, and 4- O -methylsapponal by hydrogen-1 and carbon-13 nuclear magnetic resonance. Based on the above results, the characteristic chromatogram of C . sappan L. was established, and the characteristic peaks were identified. These results provided a theoretical basis for the quality assessment of C . sappan L." @default.
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- W4285198527 date "2022-01-01" @default.
- W4285198527 modified "2023-09-26" @default.
- W4285198527 title "Isolation and identification of unstable components from <i>Caesalpinia sappan</i> by high-speed counter-current chromatography combined with preparative high-performance liquid chromatography" @default.
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- W4285198527 doi "https://doi.org/10.1515/chem-2022-0172" @default.
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