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• W4285199775 startingPage "107" @default.
• W4285199775 abstract "The mechanism of thermal death of mold conidia has not been understood in detail. The purpose of this study is to analyze the death kinetics of heated conidia of Cladosporium sphaerospermum and to ascertain the expectant cell injury responsible for the death. The death of the dormant (resting) conidia of Cladosporium sphaerospermum was examined at temperatures of between 43 and 54℃ with the conventional colony count method. The death reaction apparently followed the first order kinetics, but the Arrhenius plot of the death rate constant demonstrated seemingly a break. The linearity at temperatures higher than that at the break was lost at lower temperatures, suggesting the involvement of an unusual mechanism in the latter temperatures. In the cell morphology, we observed with quinacrine staining the vacuole rupture at a lower temperature but not at a high temperature. Interestingly, the vacuole rupture by low-temperature heating was found to correlate with the viability loss. Furthermore, active protease originally locating in vacuoles was detected in the cytoplasm of the conidia after heated at a low temperature. The results obtained suggest the involvement of potent autophagic cell death induced by low temperature heating of C. sphaerospermum conidia." @default.
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• W4285199775 date "2022-01-01" @default.
• W4285199775 modified "2023-09-30" @default.
• W4285199775 title "Low Temperature Heating-Induced Death and Vacuole Injury in<i> Cladosporium sphaerospermum</i> Conidia" @default.
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• W4285199775 doi "https://doi.org/10.4265/bio.27.107" @default.
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