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- W4285203431 abstract "Bioluminescence (BL) has been widely used to quantitatively monitor various biological phenomena. Here, we describe a protocol for preparing and using cells expressing exosomes labeled with luciferase. The BL of the culture medium of these cells is proportional to the number of secreted exosome particles obtained by well-established nanoparticle tracking analysis, allowing easy, rapid, and sensitive quantification of exosomes in vitro and in vivo. This method, designated the ExoLuc system, is a powerful tool for analyzing the molecular mechanisms of exosome biosynthesis, secretion, uptake, and biodistribution." @default.
- W4285203431 created "2022-07-14" @default.
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- W4285203431 date "2022-01-01" @default.
- W4285203431 modified "2023-10-16" @default.
- W4285203431 title "Quantification and Imaging of Exosomes via Luciferase-Fused Exosome Marker Proteins: ExoLuc System" @default.
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- W4285203431 doi "https://doi.org/10.1007/978-1-0716-2453-1_21" @default.
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