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- W4285243386 abstract "Brush cells are chemosensory epithelial cells present at most mucosal surfaces. Brush cells are a dominant source of cysteinyl leukotrienesCysteinyl leukotrienes and IL-25 in the airway epithelium and are equipped with the machinery to generate prostaglandins and acetylcholine. Activation of innate type 2 lymphoid cells and dendritic cells triggered by brush cell-derived mediators skew the immune response in the airway to type 2 inflammationInflammation that underlies atopic disease such as asthma. This chapter describes an effective method of brush cell isolation from the mouse trachea Tracheas for transcriptional analysis and from the nasal cavity for transcriptional analysis and ex vivo stimulation. The nasal or tracheal mucosa is first incubated in a dispase solution for easy mechanical separation of the epithelial layer from the underlying submucosa. The detached epithelium is then digested with a papain solution. This method provides high yields of viable brush cells in a single-cell suspensionSingle cell suspension, which can be used for flow cytometric analysis, single-cell sorting, cell culture, and functional assays. In the noseNose, where brush cells are more abundant, we present two methods of isolation of brush cells: (1) using fluorescent reporter mice that mark brush cells or (2) using a combination of high expression of EpCAM and low expression of CD45 to obtain a population of cells that is enriched for nasal chemosensory brush cells." @default.
- W4285243386 created "2022-07-14" @default.
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- W4285243386 date "2022-01-01" @default.
- W4285243386 modified "2023-09-30" @default.
- W4285243386 title "Isolation, Ex Vivo Culture, and Stimulation of Tracheal and Nasal Chemosensory Cells" @default.
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- W4285243386 doi "https://doi.org/10.1007/978-1-0716-2364-0_11" @default.
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