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- W4285256168 abstract "Intracellular reactive oxygen species (ROS) act as an important signaling transductor in cells, regulating almost every aspect of cell biology. Measurements of ROS production thus, offer links between oxidative stress and cell pathophysiology. Here, we describe a simple screening assay in intact adherent cells by fluorescence microplate readers, using dihydroethidium (DHE) and MitoSOX to measure cytosolic superoxide and mitochondrial superoxide production, respectively. This assay enables a quick and reliable assessment of ROS generation in a well-controlled environment." @default.
- W4285256168 created "2022-07-14" @default.
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- W4285256168 date "2022-01-01" @default.
- W4285256168 modified "2023-10-12" @default.
- W4285256168 title "A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX" @default.
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- W4285256168 doi "https://doi.org/10.1007/978-1-0716-2309-1_24" @default.
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